SU986396A1 - Method of determining regeneration ability of bone tissue - Google Patents

Description

(54) METHOD FOR DETERMINING THE REGENERATIVE ABILITY OF BONE TISSUE

The invention relates to medische, and he has to orthopedics. In medical practice, the degree of maturity of the archive is usually assessed by X-ray. The essence of the method lies in the fact that after a certain period of time he carried out dynamic control and the occurrence of calcium inclusions (bone tissue) judged the degree of maturity of the regenerate between bone fragments in the process of distractio osteositis by the method of A. A. Ilizarov 1. However This method of assessing the intensity of bone tissue regeneration does not often allow dynamic coitropism, since it creates an increased radiation load, while X-rays can inhibit the mitotic activity of the growing ostnoy tissue. The aim of the invention is to determine the intensity of bone tissue regeneration. The goal is achieved by the fact that during the implementation of the method for determining the regeneration capacity of bone tissue, the content of deoxyribonucleic acid is determined in red blood cells, and its content is from 11.6 ± 2.5 to 31.7 ± 3.4 ng / million red blood cells b) bone tissue regeneration, and when the content of deaconribonucleic KHcnofbi is from 7.8 ± 0.5 ng per million red blood cells and less, the completed regeneration of bone tissue is determined less. The method is carried out as follows. Blood from a vein is taken into the cooled floor by ethylene test tubes containing 3, In% - sodium citrate solution. Centrifuged at 1500 rpm, the plasma, leukocytes, and platelets are removed and crushed again with 3.8% sodium citrate solution. the number of laundered ertrotsnt count in Mlanzhery in millions per micron. After that, 0.09-0.11 ml of erythrocyte mass is applied to two disks of filter paper n fixed in a 10 15 MP mixture of 0.2 n. perchloric acid solution with 0.05 M. magnesium chloride for 10-15 minutes at 4-5 s. The mixture is drained, and the process is repeated. After that to both samples with 3986

10-15 ml of acetone is poured, heated to boiling, the acetone is poured off, and the process is repeated once more. After the acetone has been drained, 10-15 ml of a mixture of ethyl alcohol and diethyl ether are added to the erythrocytes, taken in a 3: 1 ratio, heated to boiling, and the mixture is drained. The process is repeated.

. To one disk of filter paper, containing a sample of red blood cells, 4 ml 0.3 n are added. potassium hydroxide solution and is hydrolyzed 50-70 min at 36 -

37 ° C, while the RNA is selected.

Next, 2 ml of a 0.5 M solution of perchloric acid is poured into the second disk containing a sample of erythrocyte mass, hydrolyzed for 18-22 min in a boiling water bath, and thus deoxyribonucleic acid is isolated. The hydrolyzate is cooled and 2 ml of 1.2N perchloric acid solution are added, then filtered,

The optical density of the filtrate in a cuvette of 1 cm at a wavelength of 270 Nm and 290 nm is measured on a spectrophotometer and the concentration of deoxyribonucleic acid is calculated by the formula

324E 210-790

 IG / million

WITH

P

where C is the concentration of DNA;

p - the number of erythrocytes;

E is the difference in optical density at

a wavelength of 270 nm and 290 nm. The concentration of deoxyribonucleic acid in the range of 11.6 + 2.56 - 31.78+ 3.48 ng / mdn. erythrocytes and more suggests that the process of bone tissue regeneration is intensive, and with a content of 7 ug / million, erythrocytes and less change of DNA concentration in erythrocytes during compression and osteoarthritis according to Ilnzarov.

20 6.9 0.36 22.9

The process of intensive bone tissue regeneration is completed.

Example. I examine the patient's blood, see that the red blood cell count is 5.7 million / mm.

Control.

E270 0.32;

E 290 0.295;

D E 0,32-0,295 0,025

Experience.

E „about 0,375;

E290 0.270;

D E 0,375-0,270 0,105;

D E 0,105-0,025 0,08;

Sakk. 3264 X 0.08 / 5.7 46.9 ng / million

Therefore, from human erythrocytes, 46.9 ng / million were given. erythrocyte deoxyribonucleic acid, indicating that the intensive regeneration of bone tissue.

The method was applied to 20 patients, the results of which are presented in the table and show the possibility of determining the intensity of bone tissue regeneration.

The described method has the advantage that when conducting an examination, patients are not exposed to X-rays and radioactive isotopes. The method makes it possible to estimate the intensity of bone tissue regeneration and the degree of maturity of the regenerate without the use of expensive reagents and equipment.

In addition, the method makes it possible to trace the dynamics of the regenerative process, which is very important in the prevention of clinical complications in the form of false vanity

BOB

20

20 7.8

36.9 0.4

1.9 2.1

8.4 22.8 26.0 15.0 1.1

59863966

Claims (1)

The formula of the invention of nucleic acid from 7,8tO ng / million "erit" The method of determining the regenerative method - the regeneration of bone tissue, bone tissue, the distinguishing-sources of information, so that, in order to determine the intensiveness taken into account, the tissue regeneration of costume fabrics is taken into account, in view of the 1. The transosseous compression, the blood distracts determine the content of deoxygenation and compression-distraction Bonucleic acid and its content containing osteosynthesis in traumatology and orthopedics. Scientific research institutes from 11.6 ± 2.5 to 31.713.4 gigabyte per million, erythroad-Sub. ed. Ilizarova G. A. Chel Binsk, They determine the intensive regeneration of the South Ural Book Publishing House, 1976, bone tissue, and when the content of deoxyribo-p. 10-77. rocytes and fewer determine complete