SU973612A1 - Method for producing nutrient substrate for production of fodder yeast - Google Patents

Description

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The invention relates to the microbiological industry, in particular to a method for producing a nutrient substrate from a vegetable raw material for growing forage yeast producers on it.

A known method for preparing a nutrient medium based on lignin oxide is a mixture of monobasic and dibasic organic acids used to grow fodder yeast by a known method 1.

However, the yield of yeast biomass by this method is low and amounts to 20.0 g per liter of substrate.

A known method for producing a nutrient substrate for the production of fodder yeast by hydrolysis of plant raw materials with condensed sulfuric acid, inversion, neutralization to a pH of 4.5-6.0 and separation of lignin from substrate 2.

The disadvantage of this method is the low yield of sugars (PB), which is 60% of absolutely dry wood and the yield of yeast JOT PB is 49%.

The purpose of the invention is to harvest the biological quality of the substrate by increasing the yield of carbon-containing substances and thereby increasing the biomass yield.

The goal is achieved by the fact that according to the method of obtaining a nutrient substrate for the production of fodder yeast, involving hydrolysis of vegetable raw materials with concentrated sulfuric acid, inversion, neutralization to a pH of 4.5-6.0 and separation of lignin from the substrate, the substrate after separation from lignin is evaporated 2-3 times, sodium sulfate is precipitated by adding an alcohol ether-aldehyde fraction or ethyl alcohol 5 ta in an amount of 0.8-1.0 by volume of the substrate, followed by distillation of the solvent, while neutralizing the hydrol bated until pH 4.5-6.0 is performed soda lye obtained during the preliminary alkaline hydrolysis ras20 titelnogo feed at 175-190 ° C, containing organic acids and carbonyl compounds.

Example 1. Alkaline hydrolysis of aspen wood in the form of chips is carried out in a laboratory autoclave with a capacity of 5 liters according to the following mode: hydrolysis temperature, treatment time 3 hours hydraulic module 5. Alkali consumption 20% of the weight of absolutely dry wood (in units of NajO). After processing, the liquor is removed and used to further neutralize the second stage hydrolyzate. The yield of organic acids and carbonyl compounds contained in the liquor is 31.4% by weight of absolutely dry wood. The pulp residue with a yield of 42.0% of absolutely dry wood is washed with water, suspended to an air-dry state and hydrolyzed with sulfuric acid at a concentration of 15% for 1.5 hours. After diluting the acid hydrolyzate with water to a concentration of 5% sulfuric acid, inversion is carried out oligosaccharides at 100 ° С for 5 h. The yield of monosaccharides is 45.3% from absolutely dry wood. The hydrolyzate is neutralized with lye I step to pH 6.0, the lignin precipitate is filtered, the filtrate is evaporated by volume 2-3 times. For precipitating sodium sulfate, an alcohol ether-aldema fraction (EAF) or ethyl alcohol of hydrolysis-alcohol plants in an amount of 0.8-1 to the substrate volume is added to the filtrate. The precipitation of sodium sulfate is carried out at 15-20 ° C for 1 hour. The sodium sulfate precipitate is removed by filtration or decantation. Alcohol EAF or ethyl alcohol is distilled off from the substrate, then diluted with water to a concentration of assimilable substances (monosaccharides, organic acids, carbonyl compounds) of 1%. The total yield of digestible substances is 76.7% of absolutely dry wood. Nutrients are added to the substrate; i salts of nitrogen, phosphorus and potassium by calculation, based on the content in the cell of yeast,%: nitrogen phosphorus 4; potassium 3; Sowing pressed Candida scottii KS-2 yeast on the synthetic medium of Andreev in the amount of 100% of the substances digestible by the yeast and expressing the yeast according to the generally accepted method by a periodic method with the necessary pH control. The yield of yeast 51.7% of the digestible substances, 86.3% of PB. Example 2. Alkaline hydrolysis of aspen was carried out in a laboratory autoclave with a capacity of 5 liters at a temperature of 175 ° C for 3 hours in a water ratio of 5. Alkali consumption of 15% of the weight of absolutely dry wood (in units N and O) After the processing of the liquor is taken and is used further in the neutralization of the second stage hydrolyzate. The yield of organic acids and carbonyl compounds containing in liquor is 28.5% by weight of absolutely dry wood. 2 Cellulose residue with a yield of 45.9% by weight of absolutely dry wood is washed with water, dried to an air-dry state and hydrolyzed with sulfuric acid with a concentration of 80% for 2.0 hours. After diluting the acid hydrolyzate with water to a concentration of 10% sulfuric acid wire t inversion of oligosaccharides at 100 ° C for 5 hours. The yield of monosaccharides is 43.6% from absolutely dry wood. The obtained acid hydrolyzate is neutralized with liquor of the first stage to a pH of 5.6 and the subsequent processing of the obtained substrate is carried out according to the procedure described in Example 1. The yield of assimilable substances is 72.1% of absolutely dry wood. Nutrient salts of nitrogen, phosphorus, and potassium are added to the substrate by calculation, based on the yeast content in the cell,%: nitrogen 8; phosphorus 4; Potassium 3, compressed Candida scottii KS-2 yeast, previously accumulated on the Andreev synthetic medium, in an amount of 100% of the substances digested by the yeast, and the yeast is grown by the conventional method in a periodic manner with mandatory pH control. The yield of yeast 50.7% of the digestible substances, 83.7% of PB. Example 3. Alkaline (yield of organic acids and carbonyl compounds containing lye, 31.4% by weight of absolutely dry wood, yield of cellulosic residue 42.0% by weight of absolutely dry wood) hydrolysis of aspen wood and hydrolysis of cellulose residue with concentrated sulfuric acid ( The yield of monosaccharides is 45.3% by weight of absolutely dry wood) is carried out according to the procedure described in Example 1. After inversion, at a concentration of sulfuric acid, 20% acid hydrolyzate was neutralized with a 1-step soda lye to a pH of 4.5 and further The treatment of the obtained substrate according to the procedure described in Example 1. The total consumption of yeast-assimilated substances is 76.7% of absolutely dry wood. Nutrient salts of nitrogen, phosphorus and potassium are added to the substrate by calculation, based on the yeast content in the cell,%: nitrogen 8; Phosphorus 4, potassium 3, compressed Candida scottii KS-2 yeast, previously accumulated on the Andreev synthetic medium, in an amount of 100% of the substances digested by the yeast, and the yeast is grown according to a standard method by a periodic method with an obligatory pH control. Yeast yield 41.1% of the digestible substances, 76.1% of. PB Research data on hydrolysis and yeast cultivation are presented in the table.

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The advantages of this method, as compared with the prototype, are that, to increase the biological benignity of the substrate and the yeast yield from the Sugars, saccharic acids and carbonyl compounds in the amount of 65.4 - 69.3% of PB are added to the hydrolyzate while neutralizing it with soda alkali. and thus the total yield of yeast from PB is 76.1-86.3% of PB, instead of 62-79.7% by a known method. In addition, the proposed method allows the full integrated use of wood, the yield of yeast is increased compared to the existing technology of growing yeast on hydrolysates almost 2 times and is 350-390 kg per 1 ton of wood. Dedicated lignin can be produced as a commercial product or used as fuel to produce process steam.

The introduction of the crystallization stage N82804 to the right of the substrate allows the subsequent regeneration of chemicals by electrodialysis and use them in hydrolysis cooking, i.e., create waste-free technology.

Claims (2)

1. USSR author's certificate number 335273 cl. C 12 N 1/22, -1970. 2. USSR author's certificate number 587148 cl. C 12 N 1/24, 1976.