SU959784A1 - Method of restoring antiaggregation activity of vessel walls - Google Patents

Description

The invention relates to experimental biology and medicine and can be used to study the normal and pathological physiology of the vascular wall and possible ways of changing its state.

It is known that a slight decrease in the anti-aggregation effect of the vessel wall is observed under the influence of nicotinic acid.

However, the known method does not allow to eliminate the inhibitory function of the vascular wall and activate the initial antiaggregation activity of the endothelium.

The aim of the invention is to eliminate the inhibitory function of the vascular wall and activate anti-aggregation activity, the endothelium.

This goal is achieved by the fact that phytin, glutamic acid is administered sequentially into the body, and then dipyridamole is intravenously.

, In experiments on rats under conditions of experimental decrease in an.Antigagregative properties of vascular endothelium under the influence of adrenaline. (0.04 mg / 200 g) administration of phytin, C4-8 mg / g), glutamic acid

(10–16 mg / kg) orally and then dipyridamole (0.2–0.6 mg / kg) prevented the inhibition of antiaggregation activity caused by adrenaline. Against the background of a decrease in the antiaggregation activity of the endothelium under the influence of adrenaline, the proposed method restored the antiaggregation potential of the vessel wall.

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The proposed method restores the anti-aggregation activity of LuZshe than the thromboxanesiptetase inhibitor nicotinic acid and its preparation complamine, as well as the isolated 5 applied dipyridamole. .

The results of a comparative study of the effect of various medicinal substances on the restoration of the antiaggregatory activity of the vascular wall of the wall damaged by the administration of adrenaline (0.04 mg / 200 g) are listed in the table.

Example..The first group of animals (rats) was injected with adrenaline .25 intravenously at the rate of 0.004 mg / 200 G, the second - oral phytin 7 mg / kg / glutamic acid 14 mg / kg, and after 30 min, intravenous dipyridamole at a dose of 0.42 mg / kg After 30 minutes 30, the second group of animals is injected in the same dosage as the animals of the first group. Under Nembutal anesthesia, the abdominal aorta is removed. A portion of the abdominal aorta is cut out above the bifurcapium, and drained in Tris buffer (pH 7.5) at the temperature of the ice. Before the examination, the aorta is cleaned of surrounding connective tissue, cut along and cut into strips 5-7 mm long and weighing 5-7 mg. These strips are placed in an aggregometer cuvette containing 0.5 ml of platelet-rich plasma at a concentration of 3-4x10 cells per liter for 3 minutes. After incubation at a temperature of 20-22 ° C, the pieces are removed and the aggregation capacity of the platelets, caused by ADP (10 M), is determined in the cuvette. Incubation of platelet-rich stems leads to inhibition of aggregation as compared to control, the antiaggregation activity of the lives of healthy animals averaged 31.5-0.7%, after injection of adrenaline - 15.5 - 0.72% . After not administering adrenaline, 46.0-0.3% of antiaggregatory activity of the aorta walls did not apply the proposed cijoco6. Therefore, the proposed method prevents the decrease of the antiaggregation activity of the aortic wall under the influence of adrenaline. Example 2: Adrenaline (0.004 mg / 200 g) was intravenously injected into rats. Then after 30 minutes phytin is administered orally. 7 mg / kg, glutamic acid 14 mg / kg, and more, after 30 minutes, dipyridamole 0.42 mg / kg intravenously. The control group of animals was administered only adrenshin. In the initial state, the antiaggregative activity of the aortic wall was 31.5-0.7%, after administration of adrenaline - 15.5-0.72%, after applying the proposed 48-0.7%. Therefore, the use of the inventive method contributes to the restoration of the antiaggregation activity of the aortic wall. The proposed method can be used in biomedical experiments, in particular, to study the mechanisms of the reaction of the vascular wall to various stress factors, etc.

Claims (1)

The formula of the invention 'A method for restoring the anti-aggregation activity of the vascular wall by administering drugs, characterized in that, in order to prevent the inhibitory function of the vascular wall and activate the initial anti-aggregation activity of the endothelium, phytin and glutamic acid are administered orally and then dipyridamole intravenously in animals .