SU952189A1 - Method of extracting marrow cells and preservation solution out of spongy bone transplant - Google Patents
Method of extracting marrow cells and preservation solution out of spongy bone transplant Download PDFInfo
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- SU952189A1 SU952189A1 SU802974788A SU2974788A SU952189A1 SU 952189 A1 SU952189 A1 SU 952189A1 SU 802974788 A SU802974788 A SU 802974788A SU 2974788 A SU2974788 A SU 2974788A SU 952189 A1 SU952189 A1 SU 952189A1
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- marrow cells
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- spongy bone
- preservation solution
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(5) СПОСОБ ИЗВЛЕЧЕНИЯ КЛЕТОК КОСТНОГО МОЗГА(5) METHOD FOR EXTRACTING BONE MARROW CELLS
И КОНСЕРВИРУЮЩИХ РАСТВОРОВ ИЗ ГУБЧАТОГОAND PRESERVING SOLUTIONS FROM SPRAY
КОСТНОГО ТРАНСПЛАНТАТАBONE TRANSPLANT
1one
Изобретение относитс к медицине, а именно к ортопедии и травматологии, предназначено дл обработки губчатых аллои ксеногенных костнь}х трансплантатов, консервированных различными методами (в жидких консервирующих средах, при низких и ультранизких температурах, особенно с использованием криопротекторов и др.).The invention relates to medicine, namely to orthopedics and traumatology, intended for treating spongy alloi xenogenic bone grafts preserved by various methods (in liquid preservative media, at low and ultra low temperatures, especially using cryoprotectants, etc.).
Известен способ извлечени клеток костного мозга и консервирующих растворов из губчатого костного трансплантата механическим путем 1.A known method for the extraction of bone marrow cells and preservative solutions from a spongy bone graft by mechanical means 1.
Однако этот способ не в полной мере обеспечивает извлечение клеток и консервирующего раствора из губчатых костных трансплантатов.However, this method does not fully ensure the extraction of cells and preservative solution from cancellous bone grafts.
Целью изобретени вл етс более полное извлечение клеток костного мозга и консервирующих растворов из губчатых костных трансплантатов.The aim of the invention is a more complete extraction of bone marrow cells and preservative solutions from cancellous bone grafts.
Цель достигаетс тем, что при осуществлении способа извлечени клеток костного мозга и консервирующих растворов из губчатого костного трансплантата механическим путем, провод т вакуумирование трансплантата при давлении 0,266 г/Па- 0,133 г/Па.The goal is achieved by the mechanical method of extracting bone marrow cells and preserving solutions from a spongy bone graft mechanically by evacuating the graft at a pressure of 0.266 g / Pa - 0.133 g / Pa.
Конкретным примером осуществлени способа может служить обработка губчатой костной ткани, консервированной в жидком азоте (-196°С). После размораживани мыщелков больщеберцовой кости губчатуюA specific example of the implementation of the method is the treatment of cancellous bone tissue preserved in liquid nitrogen (-196 ° C). After defrosting of the tibial condyles
5 костную ткань иссекают в форме стружек и помешают в сосуд, в котором заливают стерильным физиологическим раствором в дес тикратном объеме (например, 20 см губчатой ткани заливают 200 мл физиологического раствора). Дл удобства лучще пользоватьс мерным сосудом.5, bone tissue is excised in the form of chips and placed in a vessel in which it is poured with sterile saline in a tenfold volume (for example, 200 cm of spongy tissue is poured with 200 ml of saline). For convenience, it is better to use a measuring vessel.
Сосуд с трансплантатами в растворе помещают , под стекл нный колпак, в котором с помощью вакуумного насоса создают от ,5 рицательное давление0,266 г/Па-A vessel with grafts in a solution is placed under a glass cap, in which a negative pressure of 0.266 g / Pa is created using a vacuum pump.
0,133 г/Па и обрабатывают в течение 2- 3 мин. Затем в течение 2-3 мин уравнивают отрицательное давление воздуха в резервуаре с атмосферным. Образовавщуюс смесь содержимого чеек костной ткани и0.133 g / Pa and treated for 2–3 min. Then within 2-3 minutes equalize the negative air pressure in the tank with atmospheric. The resulting mixture of bone cell contents and
физиологического раствора в сосуде сливают . Трансплантаты в сосуде заливают новой порцией физиологического раствора и повтор ют процедуру вакуумировани . Максимальное извлечение клеток костного мозга и консервирующих растворов наступает после трехфазового проведени процедуры. saline in the vessel is drained. The grafts in the vessel are poured with a new portion of saline and the procedure for evacuation is repeated. Maximum extraction of bone marrow cells and preservative solutions occurs after a three-phase procedure.
Способ извлечени клеток костного мозга и консервирующих растворов из губчатых костных трансплантатов примен етс непосредственно при подготовке трансплантатов к пересадке перед операцией.The method of extracting bone marrow cells and preservative solutions from cancellous bone grafts is used directly in the preparation of grafts for transplantation before surgery.
Способ может быть использован дл извлечени костного мозга на этапе заготовки губчатых костных трансплантатов до помещени их в костный банк дл хранени .The method can be used to extract bone marrow during the preparation of cancellous bone grafts prior to placing them in the bone bank for storage.
Обработка губчатых костных трансплантатов предложенным способом снижает их антигенную активность, в основном определ ющуюс наличием клеток костного мозга , а также удал ет консервирующие растворы , исчерпавщие свое назначение и неThe treatment of spongy bone grafts by the proposed method reduces their antigenic activity, mainly determined by the presence of bone marrow cells, and also removes preservative solutions that are exhausted and do not
всегда благопри тно вли ющие на репаративную регенерацию тканей реципиента.always beneficial to reparative tissue regeneration of the recipient.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802974788A SU952189A1 (en) | 1980-08-19 | 1980-08-19 | Method of extracting marrow cells and preservation solution out of spongy bone transplant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802974788A SU952189A1 (en) | 1980-08-19 | 1980-08-19 | Method of extracting marrow cells and preservation solution out of spongy bone transplant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU952189A1 true SU952189A1 (en) | 1982-08-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU802974788A SU952189A1 (en) | 1980-08-19 | 1980-08-19 | Method of extracting marrow cells and preservation solution out of spongy bone transplant |
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| Country | Link |
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| SU (1) | SU952189A1 (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946437A (en) * | 1988-12-02 | 1990-08-07 | Bar-Ilan University | Method for the stimulation of bone marrow cells |
| WO1995019797A1 (en) * | 1994-01-21 | 1995-07-27 | Osteotech, Inc. | Preparation of bone for transplantation |
| US5556379A (en) * | 1994-08-19 | 1996-09-17 | Lifenet Research Foundation | Process for cleaning large bone grafts and bone grafts produced thereby |
| US5797871A (en) * | 1994-08-19 | 1998-08-25 | Lifenet Research Foundation | Ultrasonic cleaning of allograft bone |
| WO1998041245A1 (en) * | 1997-03-20 | 1998-09-24 | Osteotech, Inc. | Pressure flow system and method for treating a fluid permeable workpiece such as bone |
| US5976104A (en) * | 1994-08-19 | 1999-11-02 | Lifenet Research Foundation | Recirculation method for cleaning essentially intact bone grafts using pressure mediated flow of solutions and bone grafts produced thereby |
| US5977034A (en) * | 1994-08-19 | 1999-11-02 | Lifenet Research Foundation | Composition for cleaning bones |
| US7648676B2 (en) | 2004-04-20 | 2010-01-19 | Rti Biologics, Inc. | Process and apparatus for treating implants comprising soft tissue |
-
1980
- 1980-08-19 SU SU802974788A patent/SU952189A1/en active
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946437A (en) * | 1988-12-02 | 1990-08-07 | Bar-Ilan University | Method for the stimulation of bone marrow cells |
| US5513662A (en) * | 1991-12-31 | 1996-05-07 | Osteotech, Inc. | Preparation of bone for transplantation |
| WO1995019797A1 (en) * | 1994-01-21 | 1995-07-27 | Osteotech, Inc. | Preparation of bone for transplantation |
| AU689227B2 (en) * | 1994-01-21 | 1998-03-26 | Warsaw Orthopedic, Inc. | Preparation of bone for transplantation |
| US5556379A (en) * | 1994-08-19 | 1996-09-17 | Lifenet Research Foundation | Process for cleaning large bone grafts and bone grafts produced thereby |
| US5797871A (en) * | 1994-08-19 | 1998-08-25 | Lifenet Research Foundation | Ultrasonic cleaning of allograft bone |
| US5820581A (en) * | 1994-08-19 | 1998-10-13 | Lifenet Research Foundation | Process for cleaning large bone grafts and bone grafts produced thereby |
| US5976104A (en) * | 1994-08-19 | 1999-11-02 | Lifenet Research Foundation | Recirculation method for cleaning essentially intact bone grafts using pressure mediated flow of solutions and bone grafts produced thereby |
| US5977034A (en) * | 1994-08-19 | 1999-11-02 | Lifenet Research Foundation | Composition for cleaning bones |
| WO1998041245A1 (en) * | 1997-03-20 | 1998-09-24 | Osteotech, Inc. | Pressure flow system and method for treating a fluid permeable workpiece such as bone |
| US7648676B2 (en) | 2004-04-20 | 2010-01-19 | Rti Biologics, Inc. | Process and apparatus for treating implants comprising soft tissue |
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