SU951121A1 - Method of revealing proteins - Google Patents
Method of revealing proteins Download PDFInfo
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- SU951121A1 SU951121A1 SU803229035A SU3229035A SU951121A1 SU 951121 A1 SU951121 A1 SU 951121A1 SU 803229035 A SU803229035 A SU 803229035A SU 3229035 A SU3229035 A SU 3229035A SU 951121 A1 SU951121 A1 SU 951121A1
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- USSR - Soviet Union
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- proteins
- dye
- gels
- stained
- washing
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Description
Изобретение относитс к медицине , а именно к способам аналитической биохимии,дл обнаружени белков в полиакриламидных гел х и npiH анализу белков.The invention relates to medicine, in particular to methods of analytical biochemistry, for the detection of proteins in polyacrylamide gels and npiH protein analysis.
.Известен способ вы влени белков путем электрофоретического разделени их в полиакриламидном геле с последующим окрашиванием красителем Cll.A known method for detecting proteins is by electrophoretic separation of them in a polyacrylamide gel, followed by staining with Cll.
Однако известный способ требует длительного процесса окрашивани белковых зон в полиакриламидных гел х (ППАГ), длительного процесса отмывани избытка красител с ПЛАГ, длительного процесса отмывани гелей ведет к вымыванию минорных фракций белков, что снижает качество анализа белков, многократной смены растворител и в св зи с этим большой расход уксусной кислоты и сложного удалени фона красител , что затрудн ет денситометрирование белков.However, the known method requires a long process of staining protein zones in polyacrylamide gels (PPG), a long process of washing excess dye with PLAG, a long process of washing gels leads to washing out of minor protein fractions, which reduces the quality of protein analysis, multiple solvent changes and due to this high consumption of acetic acid and complex removal of the background of the dye, which makes it difficult to densitometry proteins.
Цель изобретени - ускорение способа .The purpose of the invention is the acceleration of the method.
.Цель достигаетс тем, что при осуществлении способа вы влени белков путем электрофоретического разделени их в iiojiiiaKpHJiaMHnHOM геле с последующим окрашиванием красителем, в качестве кра.ит.п используют 1-(5-нитро-2-тиазолилазо )-2-нафтол-3,6-дисульфокислоту .The goal is achieved by the fact that in carrying out the method of detecting proteins by electrophoretic separation of them in the iiojiiiaKpHJiaMHnHOM gel, followed by staining with a dye, 1- (5-nitro-2-thiazolylazo) -2-naphthol-3 is used as the redox, 6-disulfonic acid.
Способ реализуетс следующим образом .The method is implemented as follows.
Белки вы вл ют с помощью электрофоретического разделени их в полиакриламидном геле, после чего провод т окрашивание красителем, при этом в качестве красител примен ют 1-(510 -нитро-2-тиазолилазо)-2-нафтогт -3, б-дисульфокислотуProteins are detected by electrophoretic separation of them in a polyacrylamide gel, after which they are stained with a dye, using 1- (510-nitro-2-thiazolylazo) -2-naphtogt-3, b-disulfonic acid as a dye.
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1515
нОзЗSPD
2020
пример . Электрофорез белков .(алибумина, рибонуклеазы, гистона) в подшамидных гел х проводили .по методу Панима и Чолкли в трубках диаметром 5 мм и высотой 100 мм, а также 25 в блоках ПААГ размером (1 150«150) . После электрофореза гели окрашивали 0,2%-ным раствором 1-(5-нитро-2-тиазолилазо )-2-нафтол-3,6-дисульфокислоты . в смеси метанол-вода-уксусна an example. The electrophoresis of proteins (alibumin, ribonuclease, histone) in the podshamidnyh gels was carried out. According to the method of Panim and Chalkli in tubes with a diameter of 5 mm and a height of 100 mm, as well as 25 in blocks of PAGE in size (1,150-150). After electrophoresis, the gels were stained with a 0.2% solution of 1- (5-nitro-2-thiazolylazo) -2-naphthol-3,6-disulfonic acid. in methanol-water-acetic acid
30 кислота (5:5:1). Параллельно дл 30 acid (5: 5: 1). Parallel to
сравнени окрашивались гели 0,2%-ными растворами амидо черного 10 Б и кумасси голубого R-25.0. Применение красител дл обнаружени белков в полиакриламидных гел х позвол ет определ ть белки при концентрации 10 . После 6 мин окргииивани уже четко видны окрашенные белковые зоны в блоке ПААГ, окрашиваемом красителем, без отмывани избытка красител с гел . Предложенный способ позвол ет (Сократить врем вы влени белков до 6 мин, в то врем как при использовании известного способа требуетс 30-60 мин и 15 ч дл отмывани фона красител , предложенный способ исключает пр оцесс отмывани , снижаетComparisons were stained with gels with 0.2% amido black 10 B and Coomassie blue R-25.0. The use of a dye to detect proteins in polyacrylamide gels allows the determination of proteins at a concentration of 10. After 6 minutes of coating, the stained protein zones in the PAGE block stained with the dye are clearly visible without washing off the excess dye from the gel. The proposed method allows (Reduction of protein detection time to 6 minutes, while using the known method requires 30-60 minutes and 15 hours to wash the dye background, the proposed method eliminates the process of washing, reduces
расход растворител и повьпиает производительность .solvent consumption and improved performance.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU803229035A SU951121A1 (en) | 1980-10-29 | 1980-10-29 | Method of revealing proteins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU803229035A SU951121A1 (en) | 1980-10-29 | 1980-10-29 | Method of revealing proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU951121A1 true SU951121A1 (en) | 1982-08-15 |
Family
ID=20936088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU803229035A SU951121A1 (en) | 1980-10-29 | 1980-10-29 | Method of revealing proteins |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU951121A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0520725A2 (en) * | 1991-06-24 | 1992-12-30 | Wako Pure Chemical Industries Ltd | Acid dye staining method |
-
1980
- 1980-10-29 SU SU803229035A patent/SU951121A1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0520725A2 (en) * | 1991-06-24 | 1992-12-30 | Wako Pure Chemical Industries Ltd | Acid dye staining method |
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