SU950400A1 - Leucophoresis carrying out method - Google Patents
Leucophoresis carrying out method Download PDFInfo
- Publication number
- SU950400A1 SU950400A1 SU803212642A SU3212642A SU950400A1 SU 950400 A1 SU950400 A1 SU 950400A1 SU 803212642 A SU803212642 A SU 803212642A SU 3212642 A SU3212642 A SU 3212642A SU 950400 A1 SU950400 A1 SU 950400A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- blood
- leucophoresis
- carrying
- out method
- rpm
- Prior art date
Links
Landscapes
- External Artificial Organs (AREA)
Description
(54) СПОСОБ ПРСЖЕДЕНИЯ ЛЕЙКАФЕРЕЗА(54) METHOD OF PRECHING OF LEUKAPREESIS
1one
Изобретение относитс к гематологии и трансфузиологии и может использоватьс при фракционировании крови.This invention relates to hematology and transfusiology and can be used in the fractionation of blood.
Известен способ проведени лейкафереза путем фракционировани крови в присутствии осадител .There is a known method of conducting leukapheresis by fractionating blood in the presence of a precipitant.
Однако известный способ не обеспечивает высокого вызеоца клеток.However, the known method does not provide high cell growth.
Целью изобретени вл етс увеличение выхода клеток.The aim of the invention is to increase the yield of cells.
Цель достигаетс тем, что согласно способ проведени лейкафереза путем фракционировани крови в присутствии осадател ввод т осадитель-полиглюкин со скоростью 2,5-3,6 мл/мин, кровь фракционируют с осадителам при начальной скорости 5ОО-55О об/мин при Ktvнечной скорости вращени ротора центрифуги 75О-8ОО об/мин.The goal is achieved by the fact that according to the method of conducting leukapheresis by fractionating blood in the presence of a precipitator, a polyglucin precipitator is introduced at a rate of 2.5-3.6 ml / min, blood is fractionated from precipitators at an initial speed of 5OO-55O rpm at a Ktv of rotational speed centrifuge rotor 75О-8ОО r / min.
Способ осуществл ют слессуюишм образом .The method is performed in a slurry manner.
В экстрфюрпоральный круг кроБообращени в качестве осаоител с помощью перистальтического насоса ввод т расгвор полиглюкина со скоростью 2,53 ,О мл/мин, к которой предварительно добавл5пот 75ОО ед. гепарина.A polyglucin solution is introduced into the extrafyurpal circulating circle as a sedative by means of a peristaltic pump at a speed of 2.53, O ml / min, to which previously 75OO units are added. heparin.
Смешива сь с поступающей кровью в роторе центрифужной чаши, прсисходит разделение ее на составные фракции,Которые затем через отверсти в крышке ротора перевод тс соответственно в эритроцитарный и плазменный потсаси. Эрит- роцитарный и плазмемаый потоки, соедиto н сь, реинфузируютс донору.Mixing with the incoming blood in the rotor of the centrifuge bowl, there is a separation of it into composite fractions, which are then transferred through the holes in the rotor lid into the erythrocyte and plasma pots, respectively. The erythrocyte and plasma streams are connected and reinfused to the donor.
Начальна скорость вращени дентри гк 50О-55О об/мин. Затем по мере поступлени в циркул цию полиглюкина The initial rotational speed of the Dentry rk 50O-55O rpm. Then, as the polyglucin enters the circulation
15 скорость вращени постепегшо увелич ваетс до 75О-8ОО об/мин, что привойИт к перераспределению дел щихс и большей концентрации гранулсиитов на уровне границы плазменных и эритро20 шггаркых слоев. Скорость вз ти лейксиитов 1,О-1,5 мл/мин. В течение оп&рации лейкафереза через сепаратор клеток крови проходит 6-8 л крови.15, the rotational speed gradually increases to 75 ° -8OO rpm, which will lead to the redistribution of dividing and greater concentrations of granulesites at the level of the plasma and erythro20 layers. The rate of taking leuksiitis 1, O-1.5 ml / min. During the leukapheresis op & ration, 6–8 l of blood passes through the blood cell separator.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU803212642A SU950400A1 (en) | 1980-12-04 | 1980-12-04 | Leucophoresis carrying out method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU803212642A SU950400A1 (en) | 1980-12-04 | 1980-12-04 | Leucophoresis carrying out method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU950400A1 true SU950400A1 (en) | 1982-08-15 |
Family
ID=20929939
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU803212642A SU950400A1 (en) | 1980-12-04 | 1980-12-04 | Leucophoresis carrying out method |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU950400A1 (en) |
-
1980
- 1980-12-04 SU SU803212642A patent/SU950400A1/en active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4675117A (en) | Method of separating blood and apparatus for carrying out the method | |
| US4416654A (en) | Pheresis apparatus | |
| US4464167A (en) | Pheresis apparatus | |
| CA1298822C (en) | Continuous-loop centrifugal separator | |
| US5704889A (en) | Spillover collection of sparse components such as mononuclear cells in a centrifuge apparatus | |
| US5879280A (en) | Intermittent collection of mononuclear cells in a centrifuge apparatus | |
| AU581458B2 (en) | Closed hemapheresis system and method | |
| AU702151B2 (en) | Particle separation apparatus and method | |
| US5281342A (en) | Method and apparatus for the separation of blood into its components | |
| US5607830A (en) | Method for the continuous conditioning of a cell suspension | |
| US3703984A (en) | Method and apparatus of centrifugal separation | |
| ES8401326A1 (en) | Method and chamber for separating granulocytes from whole blood. | |
| FR2406449A1 (en) | BLOOD TREATMENT METHOD AND APPARATUS | |
| BR9806231A (en) | Systems and methods for collecting mononuclear cells using hematocrit control of clustered red blood cells | |
| JP4528863B2 (en) | Apheresis equipment | |
| SE8601153L (en) | Centrifugal separator arranged for exhaustion of a separated product with a specific concentration | |
| US4765899A (en) | Apparatus for continuous separation of leukocyte/platelet-enriched fraction from whole blood | |
| US4863609A (en) | Process for the fractional separation of protein mixtures by means of membranes | |
| SU950400A1 (en) | Leucophoresis carrying out method | |
| CN111407778A (en) | Preparation method of platelet-rich plasma | |
| US20130203582A1 (en) | Method and Apparatus for Concentrating Platelets From Platelet-Rich Plasma | |
| Klein et al. | A simple method for the separation of leukocytes from whole blood | |
| JPH02121931A (en) | Preparation of concentrated hemoglobin | |
| EP0882464A3 (en) | Method and apparatus for obtaining a platelet suspension with reduced leukocytes | |
| EP0012686A1 (en) | Isolation of hepatitis Be antigen |