SU950377A1

SU950377A1 - Method of preparing eyeball for conservation

Info

Publication number: SU950377A1
Application number: SU792871589A
Authority: SU
Inventors: Галина Георгиевна Бордюгова; Роза Александровна Гундорова; Анатолий Георгиевич Травкин; Анатолий Борисович Ципин
Original assignee: Московский научно-исследовательский институт глазных болезней им.Гельмгольца
Priority date: 1979-11-19
Filing date: 1979-11-19
Publication date: 1982-08-15

Description

1one

Изобретение относитс к медицине , а именно к трансплантологии.The invention relates to medicine, namely to transplantation.

Известен способ подготовки глазного блока перед консервацией путем стерилизации его наружной поверхности и обработки раствором диметилсульфоксида t1 .A known method of preparing the ophthalmic block before conservation by sterilizing its outer surface and processing a solution of dimethyl sulfoxide t1.

Недостатком известного способа вл четс то, что дл консервации подготавливают только изолирован- д ную роговицу, в то врем как другие элементы глаза, могущие служить трансплантатами, не используютс . Другим недостатком известного способа вл етс деформаци роговицы и j5 повреждение эндотели .The disadvantage of the known method is that only the isolated cornea is prepared for preservation, while other elements of the eye that can serve as grafts are not used. Another disadvantage of this method is corneal deformity and j5 damage to the endothelium.

Целью изобретени вл етс улучшение трансплантационных свойств тканей глазного блока путем сохранени субклеточных элементов.20The aim of the invention is to improve the transplantation properties of the tissues of the ophthalmic block by retaining the subcellular elements.

Поставленна цель достигаетс тем, что при осуществлении способа подготовки глазного блока перед консервацией путем стерилизации его наружной поверхности и обработки раствором диметилсульфоксида после обра .ботки глазного блока в стекловидное тело дополнительно ввод т 0,30 ,5 мл 11-13 -ного раствора диметилсульфоксида и влагу передней камеры замещают токоферолом.This goal is achieved by the fact that when implementing the method of preparing the eye block before preservation by sterilizing its outer surface and processing dimethyl sulfoxide solution after the eye block is processed, 0.30, 5 ml of an 11-13 solution of dimethyl sulfoxide and moisture are added to the vitreous. anterior chamber substitute tocopherol.

Способ осуществл ют следующим образом .The method is carried out as follows.

Claims

The enucleated ophthalmic block is washed with a special saline solution, immersed in a solution of dimethyl sulfoxide, in which additional processing (cleaning) of the outer tissues of the eye block is carried out. A solution of dimethyl sulfoxide is taken. Then, to reduce crystal formation in the tissues of the eye block, about 0.3-0.5 ml of a T1-13 solution of dimethyl sulfoxide is injected into the vitreous body with a syringe through a needle. After that, in order to preserve the viability of the eye tissues, approximately 0.2-0.3 mg, for example, alpha-tocopherol, with which the moisture contained therein is injected, is introduced into the anterior chamber of the eye. After this preparation of the eye block for preservation is considered complete. The proposed method was tested in the clinic. So, when transplanting through grafts obtained from ophthalmic block, canned by a known method, in 30% of cases there was a cloudy engraftment or rejection of grafts. Whereas during transplantation of grafts obtained from the ophthalmic block prepared according to the described method, similar unfavorable results were observed only in cases, out of 1007 operations performed in the last year Example f. Sick V., 55 l. He lost the vision of his left eye from childhood, after scrofula, re-transplantation of the cornea (according to the traditional wet camera method) did not give an effect. 1.5 years ago, a chemical substance appeared in the healthy eye with a perforation of the cornea, which after a long period of treatment ended in the formation of a rough, impurity wall with reduced vision to light sensation with the correct projection of light. 0.5 years ago, the patient underwent reconstructive penetrating keratoplasty with transplant graft, canned after preparation according to the described procedure. The graft adhered transparently and visual acuity O was obtained. Sick returned to social work. -Prime

2. Patient F., 40 l rubbed his eyesight as a result of burn of both eyes with lime. On the right eye, a total cornea transplantation was performed with the conjunctival plate of the eye block and vaults using the cornea, sclera and conjunctiva obtained by the described method. The results of the performed operation are favorable as a result of the transparent engraftment of the graft and the restoration of the anatomical relations of the eye; vision is restored to 0.5. Thus, with the implementation of the proposed method of preparing the eye block before conservation, the storage time of the eye block is increased by about 40-50 times, and the quality of the tissues of the eye block used for transplantation increases, i.e. tissues remain unchanged. The proposed method is more economical as it allows the use of tissues of one eyeblock for transplantation to several recipient patients. DETAILED DESCRIPTION OF THE INVENTION A method for preparing an ophthalmic block before being preserved by sterilizing its outer surface and treating a dimethyl sulfoxide solution, characterized in that. 3-0.5 ml of an 11-13 solution of dimethyl sulfoxide and moisture in the anterior chamber are replaced by tocopherol. Sources of information taken into account in the examination 1. Klin, WE. Angenheilk, 1973, p. 739.