SU950315A1

SU950315A1 - Method of determining gas and moisture concentration in expiratory air

Info

Publication number: SU950315A1
Application number: SU802889815A
Authority: SU
Inventors: Петр Васильевич Лазаренко; Ирина Петровна Воронова; Михаил Алексеевич Якименко
Original assignee: Институт Клинической И Экспериментальной Медицины Сибирского Филиала Амн Ссср
Priority date: 1980-02-22
Filing date: 1980-02-22
Publication date: 1982-08-15

Description

1one

Изобретение относитс к медицине и может быть использовано в экспериментальной биологии.The invention relates to medicine and can be used in experimental biology.

Известен способ одновременного определени концентрации газов и влаги в выдыхаемом воздухе, заключающийс в измерении концентрации газа и влаги в выдыхаемом воздухе , проход щем через распирационную камеру, до и после .воздействи JQ исследуемых факторов на организм с последующим сравнением результатов l.There is a method of simultaneously determining the concentration of gases and moisture in exhaled air, which consists in measuring the concentration of gas and moisture in exhaled air passing through the expansion chamber, before and after the JQ effect of the studied factors on the body, followed by comparison of the results of l.

Однако известный способ не позвол ет точно измерить концентрацию 15 газа при понижении температуры среды пребывани организма.However, the known method does not accurately measure the concentration of gas 15 as the temperature of the body environment of the organism decreases.

Целью изобретени вл етс повышенле точности измерени концентрации газа при понижении температуры среды 20 пребывани организма.The aim of the invention is to improve the accuracy of measuring the concentration of gas with a decrease in the temperature of the body environment 20.

Эта цель достигаетс тем, что согласно способу определени концентрации газов и влаги в выдыхаемом воздухе , заключающемус в измерении концентрации газа и влаги в выдыхаемом воздухе, проход щем через респирационную камеру, до и после воздействи исследуемых факторов на организм с последующим сравнением результатов, влажность воздуха в камере уменьща- . ют пропорционально снижению температуры окружающей среды и величины влагопотери организма в соотношени хThis goal is achieved by the fact that, according to the method for determining the concentration of gases and moisture in exhaled air, which involves measuring the concentration of gas and moisture in exhaled air passing through the respiratory chamber, before and after the effects of the studied factors on the body, followed by comparison of results, camera is reduced. are proportional to the decrease in ambient temperature and the amount of moisture loss of the body in

где V - удал емое количество влаги (мл/мин),where V is the amount of moisture being removed (ml / min),

Уц Q - количество выдел емой организмом с выдыхаемым воздухом влаги (мг/мин);A Q Q is the amount of moisture excreted by the body with exhaled air (mg / min);

- количество вод ных паров в камере (мг/л); —the amount of water vapor in the chamber (mg / l);

Q - количество проход щего через камеру воздуха (л/мин).Q is the amount of air passing through the chamber (l / min).

Способ U осуществл ют следующим образом .Method U is carried out as follows.

Claims

In the chamber with experimental animals, for example, a rat is fed atmospheric 3 air with a capacity of Q l / m through a desiccant. The air of the chamber is simultaneously pumped through another desiccant and returned to the chamber, reducing the air humidity in proportion to the decrease in the temperature of the environment and the amount of moisture loss of the body in the ratio ,,. where Vy is the amount of moisture to be removed (ml / min); The amount of moisture released by the organism with exhaled air (mg / min); f is the amount of water vapor in the chamber (mg / l); Q is the amount of air passing through the chamber (l / min). The value of Q is selected so as to maintain normal physiological conditions for the body and ensure sufficient accuracy of measurements. The calculation of the amount of moisture emitted by a living organism is made according to the formula (Q-). where Q is the amount of the chamber pumped through the air desiccant and then returned to the chamber (l / min). Since Q and Q are estimated for these conditions of experience. proportional to the readings of the analyzer. Example. The rat's weight is 350 g, the moisture analyzer reading is 2.275 mg /, 5 l / m, 8 l / min. Hence, the evaporative moisture loss per animal per minute is 2.275 mg / l (0.5+ + 3i8) l / min, 9.78 mg / min. On average, the amount of evaporative moisture loss at 4 C in laboratory rats in experiments was 24.2 t1.6 mg / min and 25.6 + 2.0 mg / m 154 at 2 C per 1 kg of weight, which is close to the values obtained gravimetrically . The proposed method provides an increase in the accuracy of measurements of concentration, gas and moisture when the temperature of the body environment of the organism drops to 0 ° C, which expands the functionality of the method and its scope. Claims of Invention A method for determining the concentration of gases and moisture in exhaled air; which consists in measuring the concentration of gas and moisture in exhaled air passing through the respiratory chamber, before and after exposure of the studied factors to the body, and then comparing the results, characterized in that, in order to improve the accuracy of measuring the concentration of gas with decreasing temperature of the body environment, the air in the chamber is reduced in proportion to the decrease in the ambient temperature and the magnitude of moisture loss of organicism in the ratios where Vu is the amount of moisture removed (ml / min); A Q Q is the amount of moisture excreted by the body with exhaled air (mg / min); —the amount of water vapor in the chamber (mg / l); Q - the amount of air passing through the chamber (l / min). Sources of information taken into account during the examination 1. Instrumentation. Vol. 5 No., p. 1962.