SU945171A1 - Culture medium for cultivating yeast - Google Patents

Description

(5) NUTRITIOUS ENVIRONMENT FOR GROWING YEAST

one

This invention relates to the microbiological industry, in particular to nutrient media for growing yeast containing waste products.

Nutrient media used for growing yeast are known, containing as source of y-hydrogen, nitrogen, mineral salts and microelements wastewater from industries processing plant raw materials, such as sulfite liquor, distillery stillage O J.

However, the use of wastewater only by the food industry narrows the raw material base for obtaining nutrient media.

The closest to the proposed technical solution to the technical essence and the achieved effect is the nutrient growth medium (and containing production waste as a source of carbon, mineral salts and trace elements and a source of nitrogen.

From production wastes, flax lobe wastes are used, and ammonium nitrate f2J is used as a source of nitrogen.

A disadvantage of the known technical solution is the low protein content in the biomass (G, 2-6, C%).

The purpose of the invention is to increase the protein content in yeast biomass.

The goal is achieved in that in a nutrient medium for growing yeast containing production waste as a source of carbon, mineral salts and microelements and a source of nitrogen, it additionally contains phosphoric acid, magnesium sulphate, chlorine potassium, zinc sulfate, iron oxide and manganese sulphate, at the same time, from the production wastes, effluent from the processing of the eye condensate is used. And, the source of nitrogen is ammonium sulphate, with the following ratio: , 15-0.5 Monium Orthophosphoric 0.10-0.5 Acid Sulfate MAG0, 01-0.02 Sodium 0.05-0.1 Potassium Chloride 0.0005-0.001 Zinc Sulfate Sulfate 0.01-OvQ5 Iron Sulfate 0.005-0.01 manganese Gas processing effluent The remainder of the condensate 8 well, when gas is extracted, ends with methanol to prevent hydrate formation in the main gas pipelines. Produced gas and gas hydrocarbon condensate are common

Waste water of the composition indicated is pretreated with sulfuric acid (concentrated) to pH 7.0-7.2, and then orthophosphoric acid is added to pH 0, which is a source of phosphorus, ammonium sulphate, magnesium sulphate, and these components are taken in the following Orthophosphorus ratio

0.1-0.5 acid

Ammonium sulfate 0.15-0.5

Magnesium sulphate 0.01-0.02

Gas processing effluent

The rest of the condensate

 In addition, sources of trace elements are also added to the nutrient medium, for example, potassium chloride, zinc sulphate, iron sulphate, manganese sulphate to their concentration in the nutrient medium, about D:

Potassium chloride

0.05-0.1

Sulphate 0.0005-0.001

zinc;

Sulphate 0.01-0.05

iron

Sulphate 0.005-0.01

manganese

The concentration of added additives depends on the initial amount of hydrocarbons and methanol in the medium. The nutrient medium is inoculated with an association — it enters the complex preparation plant (CGTP), where in the process of low-temperature separation the initial stream is divided into three phases: natural gas, unstable gas condensate and water-methanol mixture. After CCGT, the raw gas and the unstable condensate through the independent pipelines enter the gas processing plant, where the unstable gas condensate is processed into the following products: a broad fraction of light hydrocarbons, stable condensate, propane, butane. The resulting liquid waste consists of sludge water, small amounts of commercial formation water, various hydrocarbons and methanol. The latter are the main organic pollutants in sewage. The composition of wastewater gas condensate processing is presented in the table. 5 yeast, for example Hansenula polymorpha and Candida guellermondu, is cultured in the apparatus, with increased mass exchange. In the process of assimilation of hydrocarbons culture Candida guellermondH pos-. Ice provides the nutrient medium 1 with the vitamins necessary for the growth of the methane reclamation yeast Hansenula polymorpha. The maximum absorption of hydrocarbons and methanol by the yeast association used and the accumulation of significant amounts of protein in the biomass are observed. Example 1. To 20 liters of wastewater treatment gas condensate of the following composition, mg per 1 liter: methanol 5000; hydrocarbons. 500; , 0, hp 0,5; Fe 1.0; , 0; the rest is water, 20 ml of orthophosphoric acid, 25 g of ammonium sulphate, 2000 mg of magnesium sulphate, 8.0 g of potassium chloride, 50 mg of zinc sulphate, 2000 mg of ferrous sulphate, 1000 mg of manganese sulphate are added. The nutrient medium is seeded with the association of the yeast Hansenula polymorpha and Candida guel lermondif and continuous cultivation is carried out in the apparatus of intensive mass transfer at 3638 with a flow rate of 0.5 l / h, a growth rate of 0.08 h, pH 3.8-4.0. The yeast yield is 5055 0 g / l molded biomass with a protein content of 56-59%. At. In this way, the methanol concentration in the treated effluent is reduced to trace amounts, and the hydrocarbons to 0 mg / l. Example 2. To 20 liters of wastewater processing gas condensate of the following composition, mg / l: methanol 12000 hydrocarbons 750; hydrogen sulfide 3.0; K 0.0; 3.0; Fe 6.0,., The remaining water, is added 42 ml of orthophosphoric acid, 65 g of ammonium sulphate, 3000 mg of magnesium sulphate, 12 g of potassium chloride, 75 mg of zinc sulfate, 6000 mg of iron sulphate, 1500 mg of manganese sulphate. The nutrient medium is seeded with the association of the yeast Hansenula polymorpha and Candida guellermondli and is continuously cultivated in the apparatus of intensive mass transfer at 36-38 ° C flow rate of 0.5 l / h, growth rate O, 12 h, pH 3,, 0. The yield of yeast is 86-92.0 g / l of freshly biomass with a protein content of 63-68. In this case, the methanol concentration in the treated effluent is 0.02, and the hydrocarbon content is up to 80 mg / l. . Thus, the nutrient medium has several advantages over the known ones. To obtain it, gas condensate processing effluents are used, which are formed year-round and are currently not used, but are being pumped into underground horizons (using the example of the Orenburg field), which causes a risk of environmental pollution. The use of effluents for processing gas condensate solves the problem of their purification from hydrocarbons, methanol and reducing the total pollution. In addition, the protein content in biomass increases by an average of 56. Claims 1. Nutrient medium for growing yeast, containing production waste as a source of carbon, mineral salts and trace elements and a source of nitrogen, is different from the fact that, in order to increase the protein content in biomass yeast, it additionally contains phosphoric acid, magnesium sulphate, potassium chloride, zinc sulphate, iron sulphate and manganese sulphate; condensate, and as a source of nitrogen - ammonium sulphate, in the following ratio of components, OBD: Ammonium sulphate0, 15-0.5 Orthophosphoric acid0.10-0.5 Magnesium sulphate0.01-0.02 Chloride0.05-0-0, 1 Zinc sulphate 0.0005-0.001 Sulfate. iron 0,01-0,05 Manganese sulphate 0,005-0,01

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Recycling waste - Anderkofler LA, Hikkey J.

botki.gasovogo.Frodil'noe production. M., Food Condensate Else: Promizdat, t. T, p. 232-290.

Sources of information, 2. USSR author's certificate

Attenuated at the examination N 609763, cl. From 12 to 3/12, 1977

Claims (1)

Claim A nutrient medium for growing yeast, containing production waste as a source of carbon, mineral salts and trace elements and a nitrogen source, is distinguished by the fact that, in order to increase the protein content in the biomass of yeast, it additionally contains phosphoric acid, magnesium sulfate, potassium chloride, zinc sulphate, ferrous sulphate and manganese sulphate, while waste gas from the gas condensate is used from production waste, and ammonium sulphate is used as a source of nitrogen, with the following Ocean, about components. %: Sulfate Am monius 0.15-0.5 Phosphoric acid 0.10-0.5 Sulfate magnesium 0.01-0.02 Chloride potassium 0.05-0.1 Sulfate zinc 0.0005-0.001 Sulfate iron 0.01-0.05 Sulfate manganese 0.005-0.01 Refinery, gas condensate