SU94292A1 - The method of obtaining hyoscyamine and atropine - Google Patents

Description

The subject of the invention is a method for obtaining hyoscyamine alkaloids and atropine from snow collected from the node (but it is December) or grain iodized to snow by iodine, which implies that the degreased diethyl ether flour is subjected to extraction with 96 ° ethanol in an acidic medium. After filtering the alcohol extract, it is concentrated until alcohol is completely removed, protein substances are precipitated with alcohols, and the residue is dissolved in 13 µde. Thereafter, the active principles are extracted from both the acidic and the alkaline solution with chloroform.

Example 1. From.me: 1 pure grain is extracted with pure ether in the Soxhlet apparatus for b hour. After distilling off the ether, the extract is not transferred to the buxus and dried; ayut at 40 °. The resulting extract was tested 1 with i; a in skin rabbits in rabbits. The defatted material is placed in a flask with a 3-5-fold ratio of 1% complete acidified acid (5% solution) of 96-ethanol until a clearly acidic reaction (but to litmus). The mixture in the flask is heated npii 40 ° 3 days, or with a reversible cooler on boiling water iai bai 6 tea. with occasional shaking. The extract was filtered through a wrinkled filter into a Würz flask, and the residue on the filter was washed with alcohol, which was attached to the extract. The alcohol is distilled off, the residue cryniatoT in porcelain on a boiling water bath to the syrup density. The syrup is treated with 96 ° alcohol with a drop of 5 m, mixing the liquid with a glass rod, and the addition of alcohol is continued until the mixture is turbid.

The extract was again evaporated to a syrup density and dissolved in 50 ml of water. The aqueous extract, which has an acidic reaction, is filtered to remove dyes, tannins, and other substances, and is extracted 2-3 times in a separating funnel in small portions of chloroform (5 ml). After cleaning the acidic aqueous liquid with chloroform

JNo 94292

The liquids are added to aqueous ammonia before the alkaline reaction and chloroform is removed before depletion. The chloroform extracts are drained together, filtered through a dry filter and evaporated in a bottle or a beaker at room temperature.

Example 2. The crushed material (without pre-dispersed 1; irivane with ether) is extracted with 96 ° -m alcohol in a flask with reflux on a boiling water bath for 6 hours. or at 40 ° C for 2-3 days with occasional shaking. The extract is filtered and after distillation of the alcohol it thickens at 40. The extract obtained is dissolved in ether into an insoluble and soluble part. The ether-insoluble part of the alcoholic extract is dissolved in 50 ml of water. 13 one stretch (usually acidic in the case of toxic cereals) is filtered, as well as in the first example, purified from dyes and other substances, and chloroform extraction from the alkaline solution is prepared.

Subject invention

The method of producing hyoscyamine and atropin, characterized in that. that as a source of alkaloids use degreased diethyl ether, collected from under the snow, for example, in Nov-December, or crops overwintering under snow.