SU939545A1 - Method for isolating bacteria of the genus arthrobacter from active silt of aerotanks - Google Patents

Method for isolating bacteria of the genus arthrobacter from active silt of aerotanks Download PDF

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Publication number
SU939545A1
SU939545A1 SU813236136A SU3236136A SU939545A1 SU 939545 A1 SU939545 A1 SU 939545A1 SU 813236136 A SU813236136 A SU 813236136A SU 3236136 A SU3236136 A SU 3236136A SU 939545 A1 SU939545 A1 SU 939545A1
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USSR - Soviet Union
Prior art keywords
aerotanks
bacteria
isolating bacteria
genus arthrobacter
activated sludge
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SU813236136A
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Russian (ru)
Inventor
Раиса Игнатьевна Милькина
Петр Ильич Гвоздяк
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Производственное Объединение "Краснодарнефтеоргсинтез"
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Priority to SU813236136A priority Critical patent/SU939545A1/en
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Description

(5) СПОСОБ ВЫДЕЛЕНИЯ БАКТЕРИЙ РОДА ARTHROBACTER ИЗ АКТИВНОГО ИЛА (5) METHOD FOR ISOLATING BACTERIA OF ARTHROBACTER KIND FROM ACTIVE SILT

1one

Изобретение относитс  к микробиологии , в частности, к микробиологическим исследовани м активного ида в процессе биологической очистки нефтесодержащих сточных вод.This invention relates to microbiology, in particular, to microbiological studies of active ida in the process of biological treatment of oil-containing waste water.

Известен способ выделени  бактерий из активного ила аэротенков, предусматривающий приготовление суспензии активного ила в стерильной водопроводной воде и высев на агаризованную питательную среду. Затем отдельно выросшие колонии перенос т в пробирки со скошенной твердой средой . После проверки чистоты выделенных штаммов, последние подвергают детальному исследованию с целью вы влени  морфологических, культуральных и физиологических признаков, используемых при последующей видовой идентификации микробов ij.A method for isolating bacteria from activated sludge aerotanks is known, which involves preparing a suspension of activated sludge in sterile tap water and sowing on an agarized nutrient medium. Then, separately grown colonies are transferred to tubes with beveled solid media. After checking the purity of the isolated strains, the latter are subjected to a detailed study in order to identify the morphological, cultural, and physiological characteristics used in the subsequent species identification of microbes ij.

К недостаткам способа относитс  его больша  трудоемкость.The disadvantages of this method are its large labor intensity.

Ближайшим техническим решением по технической сущности и достигаемоАЭРОТЕНКОВThe closest technical solution to the technical nature and achievable AEROTENTS

му эффекту  вл етс  способ выделени  бактерий рода Arthrobacter из активного ила аэротенков, предусматривающий приготовление суспензии активного ила С 2j.This effect is a method of isolating bacteria of the genus Arthrobacter from activated sludge from aeration tanks, involving the preparation of a suspension of activated sludge C 2j.

Согласно известному способу Г мл суспензии в 1 л питательной среды дл  углеводородокисл ющих бактерий инку& руют при аэрации 7 суток дл  получени  накопительной культуры. Затем бактерии накопительной культуры пересевают на плотную среду дл  получени  изолированных колоний, каждую изолированную колонию исследуют визуально н под микроскопом, выбирают колонию по характерным морфолог гическим признакам соответствующую роду Arthrobacter и пересевают ба ггерии этой .выделенной колонии на эле к тивную среду дл  артробактерий МПА 7 глицерина.According to a known method, G ml of suspension in 1 liter of nutrient medium for hydrocarbon-acidifying bacteria is & After aeration for 7 days to obtain an accumulation culture. Then, the accumulative culture bacteria are subcultured on a dense medium to obtain isolated colonies, each isolated colony is examined visually under a microscope, a colony is selected according to the characteristic morphological characteristics corresponding to the genus Arthrobacter, and subcultured from the selected colony to the MPA 7 glycerol arthrobacterium .

Чистую культуру выдел ют из электию Ьй среды после инкубации.Pure culture was isolated from the electrochemical medium of the medium after incubation.

Claims (1)

Формула изобретенияClaim Способ выделения бактерий рода Arthrobacter из активного ила аэротенков, предусматривающий приготовле· ι ние суспензии активного ила, отличающийся тем, .что, с целью ускорения и упрощения способа, Суспензию активного ила наносят на обезжиренное предметное стекло для закрепления на нем бактерий рода Arthrobacter с помощью выделяемых восков, а затем промывают предметное стекло струей стерильной воды.A method for isolating bacteria of the Arthrobacter genus from activated sludge of aeration tanks, comprising preparing a suspension of activated sludge, characterized in that, in order to expedite and simplify the method, a Suspension of activated sludge is applied to a defatted glass slide to fix Arthrobacter bacteria on it using waxes, and then washed the slide with a jet of sterile water.
SU813236136A 1981-01-13 1981-01-13 Method for isolating bacteria of the genus arthrobacter from active silt of aerotanks SU939545A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU813236136A SU939545A1 (en) 1981-01-13 1981-01-13 Method for isolating bacteria of the genus arthrobacter from active silt of aerotanks

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU813236136A SU939545A1 (en) 1981-01-13 1981-01-13 Method for isolating bacteria of the genus arthrobacter from active silt of aerotanks

Publications (1)

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SU939545A1 true SU939545A1 (en) 1982-06-30

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