SU931188A1 - Method of producing phenol, exhibiting antiinflammatory effect - Google Patents

Description

(54) METHOD FOR OBTAINING PHENOL COMPOUNDS

OWNED INFLAMMATORY ACTION

one

The invention relates to the pharmaceutical industry and relates to the isolation of biologically active substances from vegetable raw materials.

A method of obtaining phenolic compounds with anti-inflammatory action is known by extracting plant material with an organic solvent, evaporation of the extract and purifying it tlj However, the known method does not provide a sufficiently high yield of the target product.

The purpose of the invention is the expansion of the resource base.

The goal is achieved by the fact that HTO in the method of obtaining phenolic compounds with anti-inflammatory action, by extracting plant raw materials with () organic solvent, evaporating the extract and purifying it B, raw flowers (Besogg L ..) are used as a source of raw materials, which are extracted 4 ° C with an ethyl alcohol solution at 70-80 ° C for 30-60 mm3 and with a ratio of raw material and extractant of 1: 6, the extract was cleaned with hot water.

The method consists of the following technological stages:

extraction of sedum inflorescences with 40% ethanol at 80 ° C, with a ratio of raw material and extractant of 1: 6, the infusion time of each drain is 30 minutes, the number

10 discharges 4;

purification of the condensed extract with hot water;

separation of sediment (substances of lipophilic nature) by centrifugation;

15

precipitation of polysaccharides and others. related substances 4-fold amount of 96 ethanol;

cleaning the bottom of the residue on the polyamide sorbent column with water (removing

30 hydrophilic substances) and desorption 50 ethanol ;,

Claims (1)

obtaining the finished product by evaporation of the alcoholic eluate, dissolving the residue in strong alcohol and filtering to separate alcohol-insoluble substances of non-phenolic nature and evaporation of the alcoholic filtrate, yield 2.5-2.6% of the raw material; preparation of the dosage form. Example. 300 g of uninterrupted inflorescences of the stonecrop (Sedum TnaximOW iU.) Svjbio) are extracted with 40% ethanol at 80 ° C, the ratio of raw material and extractant is 1: 6, the infusion time of each plum is 30 minutes, the number of drains is 4. First 3 times, 6 liters of extractant with a report of absorption coefficient equal to 6 MP / g of raw material; second and subsequent -. 1.8 liters of ethanol. The obtained extracts ("7l) are evaporated to a small volume (" 250-300ml "), with 1.2-1.5 liters of hot water being poured into it and left in the cold for 12 hours. The precipitate substances of lipophilic nature (chlorophyll carotenoids, etc.) are separated by centrifugation (or filtration), and the filtrate is cleaned (g; 250 ml) to further purify the skin and treat with 4-fold amount of strong 96% alcohol (cii 1 l ) to remove polysaccharides that interfere with the release of the target product. The solution is separated from the precipitate. The precipitate is washed with 7 ° C alcohol, the last is added to the basic solution and evaporated (c: 50 O ml). The extract thus purified is placed on a polyamide sorbet column filled with a strong method and washed with water (and 2.5-2.7 L) to remove Sugars, organic acids and other r-idrophilic substances of non-phenolic nature. Rinsing with water is carried out until the occurrence of phpavonoids in the eluate. After separation of the concomitant substances, the phenolic compounds causing the effect of the preparation are desorbed with 50% ethanol. The beginning and end of washing out the phonoids are controlled by cyanidone reaction and paper chromatography. The resulting eluate is evaporated to a dry residue, the last pacTBOpsnoT in strong 96% alcohol and filtered to separate non-phenolic substances insoluble in alcohol. The filtrate is evaporated to dryness, the result is a drug. The yield is 7.69 g, which is 2.56% for the dry-dried raw. Llfinonoii product is a yellow PGIp (.-) shock with a low specific odor, mountain, taste cue, easily soluble in alcohol (96%) 40%; it is difficult in water, it is poorly soluble in ethyl acetate, chloroform and practically insoluble in ether, benzene, acetone. As a result of the production of phenolic compounds with anti-inflammatory effects, the proposed method has established that not only a big stoneworm (Z. (L4.) Subir) can be used to obtain the target product, but also other phylogenetically close species of the series. . ек ц FeEeptiiom, such as purple stonecrop and others. This greatly expands the raw material base for the production of the desired drug fvavoseda. The proposed set of gtlich-. body characteristics (extractant 40 ethanol, the ratio of raw material and extractant 1: 6, extraction temperature 8 ° C, setting time for each drain 30 minutes, the number of discharges 4) increases the intensity of the extraction process, which manifests itself in an increase in the yield of active substances and Target product - the yield of the finished product (full package) is 2.5-2.6% of the BoaaymHOi-cyjcoro raw material. While the yield of the target product in the known method Oj7-l, O% of the feedstock. Consequently, the yield of the desired product in the preliminarily developed method is increased by about three times. Method It also differs in that it provides high purification of the drug from related substances, and hence its highly selective pharmacological activity. Pharmacopogram studies show that the phenolic complex of the stonecrop of large stones (fvavosed) possesses a clearly expressed anti-inflammatory activity | does not cause adverse and toxic reactions. The drug phyphocedance is recommended for treatment of inflammatory diseases, in particular in the treatment of patients with chronic diseases of the hepatobipiarity system. The use of the drug enhances the normalizing effect of therapy on the indicators reflecting the functional state of the liver and the activity of the inflammatory process. The invention of the method of obtaining phenolic compounds with anti-inflammatory. 5 9311886 action by extracting the alcohol at 70-8 raw material with an organic solution of 30-60 minutes and with a ratio of raw materials and rinse, evaporate the extract and purify the extractant 1: 6, purify the extract It is characterized in that it is conducted with hot water. . that, in order to expand the resource base, s sources of information, as a source of raw materials used in consideration in the examination flower sedum (Sedurn U.), which1. Copyright witness USSR extracted with 4O% solution of ethyl-N 555888, -kp. A 61 K 35/78, 1977.