SU923485A1 - Method of selection of swines by stability to stress-syndrom - Google Patents

Description

() METHOD OF SELECTION OF PIGS FOR RESISTANCE TO STRESS SYNDROME

Claims (3)

This invention relates to animal husbandry, in particular to the breeding of agricultural animals. There is a method for selecting animals for resistance to various adverse effects of stress factors. As a result of this method, animals, such as pigs, are susceptible to the disease by stress syndrome, and therefore to the appearance of pale soft exudative (PSE) or dry dark raft legus (DFS) mesa are excluded from further selection. Animals are divided by resistance to stress syndrome by determining the activity of the enzyme creatine phosphokinase Q. A known method for selecting pigs is based on determining the body's response to the effect of halothane narcotic gas 2. The disadvantages of this method are high mortality from malignant hyperthermia and impaired cardiovascular system in healthy people. The closest to the proposed method is a method based on determining the amount of oxygen absorbed by animals at an external temperature of 3. Absence ohm known method is the use of complex equipment and incomplete separation of animals for resistance to stress syndrome. The purpose of the invention is to increase the reliability of the method. The goal is achieved by the fact that in the method of selection of pigs for resistance to stress syndrome, including the impact of an adverse factor followed by a biological study, an injection of red blood cells having antigen A from the system A of blood groups and by the absence or absence of an adverse factor is used as an unfavorable factor. the presence of the phenomenon of immunological shock in pigs is judged on resistance to stress syndrome. Erythrocyte injection was performed by intravenous suspension in pigs with natural, incomplete anti-Ac antibodies. . In addition, the injection of red blood cells is carried out once to animals aged from 3 weeks to 3 months, in quantities. million erythrocytes on the head, at the age of months - 32.5 million red blood cells per head, at the age of 6 months. and over 65-70 million red blood cells per head. The method is carried out as follows. In order to detect stress-resistant and unstable animals in all pigs, from three weeks and older, blood samples are taken for serum to determine -natural incomplete anti-As antibodies using a known reaction - Coombs test. In the population, usually there are from 60 to 90 animals that have naturally incomplete anti-Ac antibodies with a titer of 4 to 512 in serum. These animals are further subject to separation into stress-resistant and unstable ones. In two or three pigs that are positive for the Ac antigen of system A of the blood group on the erythrocyte stroma, blood is drawn in a volume that depends on the number of animals to be studied. The blood is centrifuged at 3000 rpm for 5 minutes. Plasma sucked off. The erythrocytes of each animal are washed three times with physiological saline, each time the centrifugal fluid and suction of the supernatant. Equal volumes of erythrocytes from each animal are mixed and an equal volume of physiological saline is added to the total amount. The resulting suspension should contain 6, million erythrocyte cells in 1 ml of suspension. The concentration of red blood cells in suspension is determined by any well-known method. The appropriate dose of the suspension is injected intravenously in animals, tested for resistance to stress. To do this, the animal is fixed, the place of the puncture of the aural ear is treated with an alcohol tampon, a rubber ring is placed on the animal's ear and, after the vein is swollen, an injection needle is inserted. After the drop of blood is shown from the needle, the rubber ring is removed from the animal's ear, a syringe with a pre-set red blood cell suspension is attached to the needle and the red blood cell suspension is injected into a vein. The dose of administering the suspension to an animal aged from 3 weeks to 3 months is a million red blood cells (1.8-2 ml suspension) aged 3-6 months. - 32.5 35 million (5-5 ml of suspension), at the age of 6 months. and older - 65-70 million erythrocyte cells (ml of suspension). In animals predisposed to stress syndrome, 1-2 minutes after injection, a typical picture of immunological shock develops, which is accompanied by a drop in animals, rapid breathing, frequent heart rate, elevated rectal temperature (up to 41 ° C), red pigmentation of certain areas of the skin, sometimes defecation. Through the mines, the animals themselves come out of shock, without additional use of medicinal substances, and remain healthy in the future, without visible consequences from the stress suffered. In animals that are resistant to stress, after the introduction of a suspension of erythrocytes, the picture of posttransfusion immunological shock described above is not observed and they remain completely calm. Experiments on the separation of pigs in the stress-resistant and unstable conducted in the breeding plant Zarechie, Minsk region. On a population of 5,500 pigs of different ages, it was found that the frequency of occurrence of naturally occurring incomplete anti-AU antibodies in the blood of animals is 60-90. In addition, during the isoimmunization of 500 heads of pigs with the aim of preparing immune monospecific antisera to determine blood groups, a shock condition was observed in 30 animals. It was found that the cause of the shock response of the recipient is an over-irritation of his central nervous system as a result of interaction in the bloodstream of natural incomplete antibodies anti-AU, with a titer of from 8 to 512, with the corresponding Ac antigen of red blood cells of the donor. In order to establish the dose of erythrocyte cell injection to artificially induce immunological shock, an experiment was conducted in the Zarechie plant on pigs of different ages. Animals with a titer of naturally occurring anti-Ac antibodies from 8 to 512 were divided, depending on age, into 9 groups of 5 animals in each group: three groups at the age of acTe from 3 weeks to 3 months. (1,2,3) three groups from 3 to 6 months (, 5,6),. three groups from 6 months. and older (7,8,9 Pigs of each group were injected intravenously with a certain dose of erythrocyte suspension with the antigen Ac of the system A of blood groups on the stroma, but at any dose in one ml of the suspension contained the same number of erythrocyte cells equal to 6, 5-7 million (corresponding to the physiological norm of erythrocytes in the blood of pigs). The animals of each group were injected with the following dose of erythrocyte cells: the first — 6.57 million j (0.8–1 ml); the second — million (1.8 -2 ml.); The third - 19-21 mln. (2.8-3 ml); the fourth - 26-28 mln. 1 (3.8-4 ml); nth one - 32.5 35 mln. (, ml); - the sixth - million, (5.8-6 ml); seventh - million (7.8-8 ml); eighth - 65-70 million (9.5-10 ml); nine - 78-8 million ( 11.5-12 ml.) In the first group, 1 stress-unstable piglet and 1 with a dubious reaction were detected in the first group, 2 stress-unstable in the second, 2 stress-unstable in the third, and all stress-resistant in the fourth; in the fifth - 1 stress-unstable, in the sixth - 1 stress-unsustainable, resistant; in the seventh - 1 stress-unstable; in the eighth - 2 stress-unstable; in the girl - 2 stress-unstable. L, Based on the experience, for further work, the optimal dose of injecting a suspension of erythrocytes with Ac antigen on the stroma was chosen for one animal aged from 3 weeks to 3 months. n-l mln. (1.8-2 ml iS-S suspension); 3-6 months - 32.5-35 mln (5-5 ml); 6 months and older - 65 70 million (9.5-10 ml). In order to identify the possibility of separation of adult pigs by resistance to stress syndrome, out of 199 experimental animals belonging to the Zarechye breeding plant, previously examined for the presence of anti-Ac antibodies, 23 were selected (6 horses and 17 single sows} with an antibody titer from 8 to 512 One minute after intravenous administration of 9.5-, 10 ml of erythrocyte suspension (6,570 million cells) with Ac antigen to these animals, 8 () pigs had a clear picture of immunological shock on the stroma, which indicates that these 8 adults pigs are predisposed to stre s-syndrome. The remaining 15 animals did not show any reactions after intravenous administration of the erythrocyte suspension. Consequently, they were resistant to stress syndrome. To test the effectiveness of the separation of young pigs for resistance to stress syndrome 6 animals of 3 weeks of age , having a blood titer of naturally occurring incomplete antibodies from 8 to 6, were injected intravenously with TZ-N million, erythrocyte cells with Ac antigen on the stroma. Of the forty-six heads of youngsters, only 5 (10.8) had a clear clinical picture of immunological shock. All young to the experimental group (46 heads) and control (30 heads) were subjected to a test for resistance to stress syndrome with the help of 3 min of halothane oil. As a result of using this test, no animal predisposed to stress syndrome was identified. Both adult and young pigs, examined for stress, resistance with the help of mild immunological shock, after testing, remained perfectly healthy through subsequent growth and development. Thus, the use of the proposed method of separation of pigs for resistance to stress syndrome provides several advantages over the known one, since the new method is more reliable in separation (more sensitive, safe for the health of animals and can be used to separate adult pigs. In addition Moreover, exclusion from selection of stress-unstable animals will allow avoiding losses in meat production, some are 10% at slaughter in m.kombinaty, due to the reduction of meat of pale soft exudate (PSE) and dry dark dense (DFD), derived from stress-unstable pigs. An additional profit per 1000 heads of pigs slaughtered at the mill is rub (at a cost of 1 kg of sausage swine 1, 7 rubles). The method of dividing pigs for resistance to stress synom. can be applied immediately. When breeding animals on the breeding farms of collective and state farms, the farms of large industrial complexes for the production of pork. Claims 1. A method for selecting pigs for resistance to stress SINDrom, including exposure to an adverse factor followed by a biological study, characterized in that, in order to increase the reliability of the method, 85 injections are used as an adverse factor. the surface of the Ac antigen of system A of blood groups and, in the absence or presence of the phenomenon of immunological shock in pigs, judged resistance to stress syndrome. 2. Method pop.1, which is also distinguished by the fact that the injection of red blood cells is carried out by intravenous administration of a pig pig suspension with natural incomplete anti-Ac antibodies. 3. Method according to paragraphs. 1 and 2, which is based on the fact that the erythrocytes are injected once by animals from 3 weeks to 3 months. in the amount of 13-1 million red blood cells per head, at the age of months. 32, 0 million erythrocytes on holoZU, at the age of 6 months. and over 65-70 million red blood cells per head. Sources of information taken into account in the examination 1.Wolf Netal But med 3-3, 1970, 386. 2. USSR author's certificate No. 782781, A 01 K 67/00, priority 30.03.79. 3. USSR author's certificate number 377138, cl. A 01 K 67/00, 1973