SU914508A1 - Process for purifying effluents from organic compounds - Google Patents

Description

The invention relates to methods for the treatment of wastewater from phenolic compounds, aromatic amines and can be used in the treatment of wastewater enterprises of oil and coal chemical, pulp and paper, metallurgical, organic synthesis and other industries.

Known methods for biological treatment of wastewater by introducing enzyme preparations ¹ gidrolitiches- anyone proteolytic action [ί] ._

The decomposition of organic compounds occurs in sewage treatment plants in two stages. The first stage is the decomposition of high-molecular-weight organic compounds, carried out with the help of hydrolytic enzymes. In the second stage, proceeding with the help of redox enzymes, the complete mineralization of organic compounds occurs.

There is also known a method for treating wastewaters of dairy plants, s.oder2.

Mouthing lactose, by digestion with galactosidase or lactose oxidase enzymes, produced by aerobic proteolytic microorganisms [2].

However, its implementation requires large expenditures on maintaining the culture of microorganisms, separating the sediment, which is unprofitable.

The closest to the proposed technical essence is a method of wastewater treatment from organic and inorganic compounds with hydrogen peroxide 1E7.

The disadvantage of this method is the low degree of purification and duration of the process (55 min, 90%), high consumption of hydrogen peroxide 2.53.0 mg / mg of contamination, the need to pre-lower the pH of the water to 3-5 units. The process is carried out in a corrosive environment, and the need to carry out the process at elevated temperatures and the introduction of purified water

914508

catalyst (salts of iron, nickel, aluminum) causes secondary contamination of water with metal ions.

The purpose of the invention is to increase the degree of purification and simplify the process. _five

This goal is achieved by the fact that the waste water is treated with hydrogen peroxide, and then further passed through the enzyme peroxidase, immobilized on silochrome.

Hydrogen peroxide is introduced in an amount of 0.04-0.4 mg per mg of contamination and passes them through a layer of silochrome with immobilized peroxidase at a concentration of 1-10 mg per 1 g of sorbent. • Treatment is carried out at a normal temperature, temperature and pressure.

The catalyst used in the work is prepared by mixing a sample of 1 g of industrial sorbent - silochrome C-120 (fraction 0.1-0.2) with a solution of 1: 5 or 10 mg peroxidase (a commercial preparation of the company "Vezla 1" Hungary) in 50 ml distilled water, incubated $ ₂ in a refrigerator at preferably 24-48 hours. At the end of the exposure, the solution is separated, the silochrome is washed with water, transferred to a column and used. The amount of sorbed peroxidase determined spectrophotometrically.

Example 1. To 100 ml of water containing 110 mg of pyrocatechin, add 0.1 ml of 30% ι through a column with 10 mg of peroxidase immobilized on silochrome. At the exit from the column _{(there are} no phenols in the water column. The obtained samples immobilized on silochrome peroxidase have high stability, which allows them to be reused.

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Example 2. A column of 10 mg of peroxidase immobilized per 1 g of silochrome is passed sequentially (in 17 cycles of 100 ml).

1700 ml of water containing 1870 mg of pyrocatechin, and 510 mg of hydrogen peroxide. The oxidizing ability of the sample used does not differ 50 from the initial one and only in the 18th cycle a decrease in the degree of purification by 68% was observed.

Example 3. To 100 ml of water containing 18.4 mg of benzidine, 55 ml of 30% H ^ O ^ are added, passed through a column, 10 mg of peroxidase immobilized with 1 g of silochrome are formed. Benzidine is absent from treated water.

Example 4. Through a column with 40 mg of peroxidase immobilized on 4 g of silochrome, 6 l (portions of 1 l) of untreated sewage (pH 7.7) of the Baikal pulp and paper mill with the addition of 6 ml of 30% hydrogen peroxide are passed through. At the exit from the column, the degree of purification from phenols was 96-97.5%. The residual phenol content was determined by gas-liquid chromatography on a Tsvet-110 chromatograph.

The experimental data on the degree of purification of water from pyrocatechin, depending on the content of hydrogen peroxide at a catalyst of 10 mg of peroxidase immobilized on a silochrome, are given in Table 1. /

Table 1

Content perekini- Degree of cleaning,% hydrogen si (ml 30% H ^ (calculating 1l 10 pyrocatechin Sn, w * hm 0.01 50 0.1 94 0.5 96 one 100 2 100 five 100 ten 100

Table 2 presents data on the degree of water purification from pyrocatechol depending on the content of peroxidase with the introduction of 1 ml of 30% per Gd 1 (G ^ M pyrocatechin

Table 2

Peroxidase content, mg / 1 g of silo-chromium Cleaning degree % 0.1 53 one 85 five 97 ten 100 20 100

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The proposed cleaning method is based on the fact that, for the purpose.

It allows to increase the degree of purification from organic compounds by 107 compared to the known, to reduce the consumption of hydrogen peroxide from 5 2.5-3.0 mg / mg of pollution in a known way to 0.04-0.4 mg / mg of pollution. With such a small consumption of hydrogen peroxide, the cost price of which is very low, and the small consumption of the enzyme, it is possible to achieve complete wastewater treatment from organic compounds (phenol, aromatic amines). In addition, the method allows to carry out the process at normal, 5 temperature and pH 6.8 - 8.0, the used catalyst - peroxidase, immobilized on the silochrome, is easily separated and does not pose a threat of secondary water pollution, and can also be repeatedly used in cleaning.

Claims (3)

Claim 23 ! The method of purification of wastewater from organic compounds by treatment with hydrogen peroxide, otlipovysheniya degree of purification n simplify the process, wastewater, water additionally Pass through the enzyme peroxidase immobilized on silochrome. 2. The method of pop. 1, which is based on the fact that hydrogen peroxide is introduced in the amount of 0.04-0.4 mg per mg of contamination and, peroxidase in the amount of 1-10 mg, immobilized 1 g of silochrome. 3. Method pop.1, characterized in that the process is conducted. at pH 6.8-8.0 and a temperature of 18-22 ° C.