SU907430A1

SU907430A1 - Device for sampling biological liquid upper zone after sedimentation

Info

Publication number: SU907430A1
Application number: SU772536601A
Authority: SU
Inventors: Валерий Леонидович Семенов; Алексей Алексеевич Биркун (Младший)
Original assignee: Научно-Исследовательский Институт Физических Методов Лечения И Медицинской Климатологии Им.И.М.Сеченова
Priority date: 1977-10-24
Filing date: 1977-10-24
Publication date: 1982-02-23

Description

Изобретение относится к биологии и медицине и может быть использовано в клинической и экспериментальной лабораторной практике для получения после седиментации возможно менее загрязненных верхних зон биологических ⁵жидкостей.The invention relates to biology and medicine and can be used in clinical and experimental laboratory practice to obtain, after sedimentation, possibly less contaminated upper zones of biological ⁵ fluids.

Известно устройство, содержащее цилиндр с канюлей и крышкой, внутри которого размещен поршень на штоке, оснащенном резьбой и гайкой [1].A device is known comprising a cylinder with a cannula and a cap, inside of which a piston is placed on a rod equipped with a thread and a nut [1].

Недостатком известного устройства является то, что извлекаемая фаза загрязняется в силу ряда причин: извлечение происходит через канюлю, загряз-₁₅ненную при наборе маточной жидкости; шток поршня приводится в движение непосредственно пальцем, что ведет к перемешиванию жидкости.The disadvantage of this device is that the phase of the extracted polluted by a number of reasons: the recovery is through a cannula, contamination ₁₅ nennuyu when dialing the mother liquor; The piston rod is driven directly by the finger, which leads to fluid mixing.

Цель изобретения - повышение одно- jq родности верхней зоны жидкостей при полном ее извлечении.The purpose of the invention is to increase the uniformity of the upper zone of liquids with its complete extraction.

Поставленная цель достигается тем, что верхний конец цилиндра оснащен съемной головкой с канюлей , на которую насажен прозрачный капилляр,внутри цилиндра между поршнем и крышкой размещена пружина, при этом цилиндр установлен неподвижно с соблюдением вертикального положения.This goal is achieved by the fact that the upper end of the cylinder is equipped with a removable head with a cannula, on which a transparent capillary is mounted, a spring is placed between the piston and the cover, while the cylinder is fixedly installed in a vertical position.

На чертеже показано предлагаемое устройство, Общий вид.The drawing shows the proposed device, General view.

Устройство содержит цилиндр 1 с крышкой 2 и размещенным внутри цилиндра поршнем 3 на штоке 4, оснащенном резьбой. На противоположном от крышки 2 (верхнем) конце цилиндра закреплена съемная головка 5, имеющая канюлю 6, на которую насажен гибкий прозрачный капилляр 7· Внутри цилиндра между поршнем и крышкой размещена пружина 8. На штоке вне цилиндра расположена гайба (бегунок) 9 и прокладка 10. Противоположный от поршня конец штока вертикально жестко закреплен в станине 11, горизонтальное положение которой контролирует уровень 12.The device comprises a cylinder 1 with a cover 2 and a piston 3 located inside the cylinder on a rod 4 equipped with a thread. On the opposite end of the cylinder 2 from the cover 2 (upper), a removable head 5 is fixed having a cannula 6 on which a flexible transparent capillary 7 is mounted. Inside the cylinder, a spring 8 is placed between the piston and the cover. A nut (slider) 9 and a gasket 10 are located outside the cylinder The opposite end of the rod from the piston is vertically rigidly fixed in the frame 11, the horizontal position of which controls level 12.

Устройство работает следующим образом.The device operates as follows.

Для седиментации отстаиванием после установки устройства в вертикальное положение цилиндр 1 при снятой головке 5 заполняется биологической жидкостью, например, кровью, с последующим закреплением плотно и соосно с цилиндром съемной головки 5 посредством ее замка.For sedimentation by sedimentation after installing the device in a vertical position, the cylinder 1 with the removed head 5 is filled with biological fluid, for example, blood, followed by fixing tightly and coaxially with the cylinder of the removable head 5 by means of its lock.

На канюлю 6 предварительно насажен капилляр 7. В случае центрифугирования, установку устройства на станину производят после окончания седиментации, например, подвижного эритроцитарного осадка и плазменного слоя, верхнюю зону извлекают путем вращения гайки ί, бегунка) 9 на штоке 4, вследствие чего усилие пружины приводит к плавному поступательному движению цилиндра 1 вниз и вытеснению отбираемой из него зоны через капилляр 7· Отбор контролируется по движению жидкости в капилляре.A capillary 7 is preliminarily inserted on cannula 6. In the case of centrifugation, the device is installed on the bed after sedimentation, for example, a movable erythrocyte sediment and a plasma layer is completed, the upper zone is removed by rotating the nut ί, slider) 9 on the rod 4, as a result of which the spring force causes to the smooth progressive movement of the cylinder 1 downwards and the displacement of the zone taken from it through the capillary 7 · The selection is controlled by the movement of the liquid in the capillary.

В качестве материала для изготовления деталей устройства, соприкасающихся с биологической жидкостью (цилиндр, поршень, съемная головка с канюлей, капилляр) , может быть ис~As a material for the manufacture of device parts in contact with biological fluid (cylinder, piston, removable head with cannula, capillary), can be used ~

907430 4 пользован прозрачный полимерный материал, например плексиглас. Устройство может содержать прокладку между крышкой и пайкой (бе5 гунком), градуировку на цилиндре и/ /или на штоке поршня.907430 4 uses a transparent polymeric material, for example plexiglass. The device may include a gasket between the cap and soldering (screwless), graduation on the cylinder and / / or on the piston rod.

Claims

(5) DEVICE FOR SELECTION OF TOP ZONES OF BIOLOGICAL FLUIDS AFTER The invention relates to biology and medicine and can be used in clinical and experimental laboratory practice to obtain, after sedimentation, possibly less contaminated upper zones of biological fluids. It is known a device comprising a cylinder with a cannula and a lid within which a piston is placed on a uiTOKe equipped with a thread and a nut 1. A disadvantage of the known device is that the extractable phase is contaminated due to a number of reasons: when recruiting uterine "liquid; The piston rod is driven directly by the finger, which leads to the mixing of the fluid. The purpose of the invention is to improve the uniformity of the upper zone of liquids with its complete extraction. The goal is achieved by the fact that the upper end of the cylinder is equipped with a SEDIMENTATION removable head with a cannula onto which a transparent capillary is mounted, a spring is placed inside the cylinder between the piston and the cover, while the cylinder is fixed in a vertical position. The drawing shows the proposed device, view. The device comprises a cylinder 1 with a cover 2 and a piston 3 placed inside the cylinder on a rod C equipped with threads. A removable head 5 having a cannula 6 is fastened on the opposite (top) end of the cylinder 2, on which a flexible transparent capillary J is fitted. A spring 8 is placed inside the cylinder between the piston and the cover. . The end of the rod opposite from the piston is vertically rigidly fixed in the frame 11, the horizontal position of which controls the level 12. 3 The device works as follows. For sedimentation by settling, after installation of the device in a vertical position, cylinder 1 with the removed head 5 is filled with biological fluid, for example, blood, followed by fixing tightly and coaxially with the cylinder of the holoderm 5 through its lock. On cannula 6, pr1; capillary 7 dually; In the case of centrifugation, the device is installed on the station after the end of sedimentation, for example, a mobile erythrocyte sediment and plasma layer, the upper zone is removed by rotating the nut ( as a result, the spring force causes the cylinder 1 to move smoothly downward and displace the zone taken out of it through the capillary 7. The selection is controlled by the movement of the liquid in the capillary. As a material for the manufacture of parts of the device, When contacting with a biological fluid (cylinder, piston, removable head with cannula, capillary), a transparent polymeric material, such as Plexiglas, can be used. The device may contain a gasket between the lid and the soldering (slider), graduation on the cylinder and / / or on the piston rod. Apparatus of the Invention A device for sampling an upper zone of biological fluids after sedimentation, comprising a cylinder with a lid within which a piston is placed on a rod equipped with a thread and a nut, in order to increase the uniformity of the upper zone of the liquid when it is removed, the cylinder is equipped with a removable head with a cannula, on which a transparent capillary is mounted, a spring is placed inside the cylinder between the piston and the cover, while the cylinder is fixed in a vertical position. Sources of information taken into account during the examination 1. USSR Author's Certificate No. 20302, cl. A 61 M 3/00, 197.