SU893179A1 - Method of determining vegetable tissue vitality - Google Patents

Description

(5) METHOD FOR DETERMINING THE VIABILITY OF PLANT TISSUES

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This invention relates to plant electrophysiology for diagnosing a functional state of a plant.

There is a method for determining the viability of plant tissue, which includes measuring electrical conductivity using electrodes l.

However, the known method is not accurate enough and is not reproducible to measure the viability of the same plant tissue.

The aim of the invention is to improve the accuracy and reproducibility of measurement results when exposed to tissue electric current.

This goal is achieved by simultaneously acting on the plant tissue with direct current and measuring the electrical conductivity of the tissue in an alternating current and judging via the tissue viability at the minimum electrical conductivity.

Fig. 1 shows a functional diagram of a measuring device that implements the proposed method, and Fig. 2 shows a curve of the dependence of the electrical resistance of the tissue on the time of the action of the electric current at a fixed value of the applied voltage.

The measurement installation contains a generator of 1 sinusoidal oscillations, a high-voltage source 2, an electronic switch 3, electrodes 4 and 5 inserted into the plant tissue 6, as well as a selective amplifier 7, a peak voltmeter 8, a reference resistor 9 and a switch 10.

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The essence of the proposed method is as follows.

After insertion of the electrodes and 5 into the test tissue 6, the initial resistance is measured initially.

Claims (1)

20 tissue Rjt by means of a impedance meter operating on the voltmeter principle. For this, the alternating voltage from the generator 1 3 through the switch 3 is supplied to a divider formed by the resistance of the fabric Ry and the reference resistor 9 having resistance Rg. The voltage drop is disproportionate to Ry and is measured by a peak voltmeter 8, the scale of which is calibrated directly to the resistance values Ry-. Then, a high voltage source 2 (within 100 V) is connected to the second input of the switch 3 via a switch 10. Switch 3 operating at a frequency of about 50100 Hz separates two processes in time: the first 5-10 ms is the measurement of Ry, the next ms of tissue heating, the next interval At is the measurement of R again, and so on throughout the entire measuring cycle. To eliminate the influence of switching noise on the measurement results, a selective amplifier 7 tuned to the generator frequency (kHz) was inserted into the installation. As a high-voltage current passes, the tissue heats up, the exoosmos of the intracellular electrolyte begins, the resistance of the intercellular pathways decreases, and the current flow increases, which (L turns out to increase exo-osmosis with a subsequent decrease in resistance. As a result, an avalanche process occurs (Fig. 2 ), in which the resistance sharply reaches a minimum value (which corresponds to the complete thermal breakdown of cell membranes, i.e. the so-called altered state). At the subsequent moment in As the resistance begins to increase rapidly due to intense evaporation of the intercellular electrolyte due to excessive heating, the minimum resistance R is fixed by a peak volt meter, which remembers the maximum voltage drop on the reference resistor 9 resistor 9 for read time, which corresponds to the mini: maximum value Resistances Next, the viability coefficient, equal to the ratio Hho Xm-tM, is determined by the calculation of the viability of the plant. The latter operation can be easily automated by introducing into the proposed installation of the storage nodes the values of Ry and RY - and a pip scheme that implements the function K. - R .. / R The same XQ X U11C where the coefficient of viability. Using the proposed method allows you to significantly speed up and fully automate the measurement process, eliminates the need to take a sample and thereby injure the plant, improves the measurement accuracy due to less disruption of the organic integrity of the area under study and allows you to accurately meter the transmitted energy. Claims The method of determining the viability of plant tissue, including measurement of electrical conductivity using electrodes, characterized in that, in order to increase the efficiency, accuracy and reproducibility of measurement results when exposed to tissue by electric current, simultaneously act on the plant tissue and direct current of tissue. on alternating current and mini; The maximum electrical conductivity is judged on the viability of the fabric. Sources of information taken into account in the examination 1. Lime Academy of Sciences of the Armenian SSR, 1958, XI, 4, p.89-95 (prototype). eight sixteen t i / f fff 8 s f g -I- q 9, i / to