SU870357A1 - Method of control of waste water biochemical purification process - Google Patents

Description

(54) METHOD OF CONTROL OF THE PROCESS OF BIOCHEMICAL CLEANING OF WASTE EC

The invention relates to the field of wastewater treatment and can be used in biochemical purification facilities for technological control of oxyteps and aeration tanks. A method of technological control of aerotanks operation is known, which consists in determining the biochemical oxygen demand (BOD) of wastewater before and after cleaning. The fact that the result of the analysis is obtained is only five days (BOD5) or the twentieth days (BOD 20), which limits it. use for operational production control. There is also known a method of technological control over the process of biochemistry purification by determining DO314 active sludge. The disadvantages of this method are the duration of the analysis, simultaneous determination with cells; the biomass of suspended matter found in the sludge suspension; the impossibility of registering the degree of destruction of the biomass. The closest to the claimed technical essence and the achieved effect is the method of technological control of the process of biochemical wastewater treatment by determining the percentage ratio of living and dead microorganisms. According to the indicated correlation, the viability of biomass of activated sludge is directly judged and indirectly when obtaining the results of two tests on the degree of its destruction. 3J and the impossibility of a single measurement of the degree of cell destruction. The aim of the invention is to speed up and simplify the analysis, as long as the accuracy of the control is increased. This goal is achieved by using the protein index as a reference indicator, to determine which pre-activated activated sludge is divided into biomass and cell-free liquid, the protein content of the obtained samples is measured by the Lowry method and the ratio of the amount of protein-free cell protein to biomass protein t about degree

cell destruction of biomass of activated sludge.

From the scientific and technical literature, various methods are known for determining cell viability and, at the same time, oTcyTCTBVwT data on direct methods for determining the degree of biomass destruction, which would be used for the operational control of the biochemical wastewater treatment process. "

Example. On the laboratory ok. The sieve is cleaned of polluted wastewater, which is produced by processing hydrogen sulfide gas at the Orenburg gas processing plant.

A sample of activated sludge is taken from the oxytile reactor and measured in it by a non-oleometric method at FEK60m cell concentration, g / l. Transfer 5.0 L of sludge slurry to two centrifuge tubes and centrifuge for 5 minutes at 5000 rpm. The resulting cell-free liquid is drained, and the amount of 280 is measured in the precipitate.

140

2.9

48

480

250

2.6

43

930

480

1.4

22

3850

1700

0.61

COD - chemical oxygen consumption VPK - biochemical oxygen consumption

protein by the method of Lowry. In addition, the protein content is determined in the sample of untreated wastewater.

To calculate the degree of destruction of the biomass of activated sludge, the difference in the amount of protein in the cell-free fluid and the crude effluent (which can also give a color reaction with Folin's reagent) is preliminarily determined, and then the ratio of the obtained value to the protein is determined. biomass (cell sediment).

The previously measured concentration of sludge cell cells allows one to additionally calculate such parameters as the amount of protein per unit dry weight of biomass and the percentage of protein to dry weight, which are also used to monitor the status of activated sludge, i.e. monitoring the process of biochemical wastewater treatment.

The performance of the oksitenka according to the known and proposed control methods is tabulated.

68.4 668.1 0.10

131.7 601.7 0.21

323.0 405.0 0.79

Claims (3)

567.3 161.3 3.51 The results of the conducted studies show that upon receipt of biochemically oxidizable effluents for purification, the protein index varies in the range of 0.05-0.15. An increase in this indicator indicates an increased destruction of biomass cells when exposed to active or toxic components of wastewater or large quantities of incoming contaminants. Therefore, the proposed control indicator reflects the degree of cell destruction of the activated sludge biomass during wastewater treatment. The proposed method makes it possible, in comparison with the known method, to shorten the analysis time (up to 30 min) and to record even a minor damaging effect of the effluent. The technical and economic effect of using the invention will be determined by increasing the efficiency of the purification process (increasing the biomass of activated sludge, improving the quality of treated effluents as a result of more rapid registration and accounting for the effect of toxic components on the silt and salvage discharges of scaffolds. informative and therefore there are no obstacles for its use in technological control at biological treatment facilities of various enterprises. Claims The method of controlling the biochemical wastewater treatment process, based on determining the degree of cell destruction of the biomass of activated sludge, characterized in that, in order to speed up and simplify the analysis, to increase the control accuracy, the activated sludge is divided into a cell-free liquid and biomass, the protein content in the resulting samples and the ratio of the amount of protein in the cell-free liquid to the biomass protein are judged on the degree of destruction of the biomass cells of the activated sludge. Sources of information taken into account in the examination 1.Metodika technological control of the sewage treatment plants. M., stroiizdat, 1977. with. 60-65. 2. Technique of technological control of the sewage treatment plant. M., Stroynadat, 1977, p. 172. 3. Recommendations for the application of the method of direct microorganism counting in technical microbiology of water. M., VNIIVODGEO, 1974, p. 5, 20-21 (prototype).