SU843897A1 - Medium for extraction of sex secretion from accessory glands of aedes aegypti mosquito males - Google Patents
Medium for extraction of sex secretion from accessory glands of aedes aegypti mosquito males Download PDFInfo
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- SU843897A1 SU843897A1 SU792817823A SU2817823A SU843897A1 SU 843897 A1 SU843897 A1 SU 843897A1 SU 792817823 A SU792817823 A SU 792817823A SU 2817823 A SU2817823 A SU 2817823A SU 843897 A1 SU843897 A1 SU 843897A1
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- secretion
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- glands
- sex
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Description
Результат: количество белка секрета, экстрагированного из придаточных желез раствором 1,28 М NaCl, составило 0,78 мкг на особь.Result: the amount of secretion protein extracted from the accessory glands with a solution of 1.28 M NaCl was 0.78 µg per individual.
Пример 2. Экстракци полового секрета из придаточных желез некопулировавших самцов А, aegypti 4-дневного возраста дистиллированной водой.Example 2. Extraction of genital secretion from the accessory glands of non-copulating males A, 4-day-old aegypti, with distilled water.
Выделили 25 пар придаточных желез в растворе «аедес, выделенные железы поместили в микропробирку с 25-30 мкл дистиллированной воды. Дальнейша носледовательность действий, как в примере 1, с использованием дистиллированной воды вместо l,28MNaCl.25 pairs of accessory glands were isolated in the aedes solution, the isolated glands were placed in a microtube with 25-30 μl of distilled water. A further sequence of actions as in example 1, using distilled water instead of l, 28MNaCl.
Результат: количество белка секрета, экстрагированного из придаточных желез дистиллированной водой, составило 0,53 мкг на особь.Result: the amount of secretion protein extracted from the accessory glands with distilled water was 0.53 µg per individual.
Пример 3. Экстракци полового секрета из придаточных желез некопулировавшнх самцов А. aegypti 4-д11евного возраста раствором «аедес.Example 3. Extraction of the genital secretion from the accessory glands of noncopulated male A. aegypti 4-year-old age with a solution of “ades.
Выделили 25 пар нридаточных желез, выделенные железы поместили в микропробирку с 25-30 мкл раствора «аедес. Дальнейша последовательность действий, как и примере 1, с использованием раствора «аедес вместо 1,28 М NaCl.25 pairs of nitrile glands were isolated, the isolated glands were placed in a microtube with 25-30 μl of the solution “ades. A further sequence of actions, as in Example 1, using the solution “ades instead of 1.28 M NaCl.
Результат: количество белка секрета, изолированного из придаточных желез раствором «аедес, составило 0,55 мкг на особь.Result: the amount of protein secretion isolated from the accessory glands with a solution of aedes was 0.55 μg per individual.
Количество белка полового секрета, экстрагированного из нрндаточных желез девственных самцов Aedes aegypti 4-днеБНого возраста различными средами, представлено-в таблице.The amount of genital secretion protein extracted from virgin glands of virgin males Aedes aegypti 4-day old by various media is presented in the table.
Предлагаема среда дл экстракции полового секрета из изолированных придаточных желез самцов насекомых обеспечивает по сравнению с сушествующими средами увеличение более чем на 40% (по белку) выхода секрета, повышение точности, воспроизводимости и достоверности результатов . Использование данной среды делает возможным разработать метод количественной оценки секреторной активности придаточных желез, позвол ет сократить число эксперимеитов и расход насекомых, необходимых ДЛ5Г получени достоверных результатов , а также обеспечивает возможность расширени биохимических н токсикологических исследований на изолированных половых железах насекомых, что имеет важное значение при разработке методов борьбы с насекомыми и методов стандарти ации лабораюрного культивировани их.The proposed medium for the extraction of sexual secretion from the isolated adnexal glands of male insects provides an increase of more than 40% (by protein) of secretion compared to existing media, increasing the accuracy, reproducibility and reliability of the results. The use of this medium makes it possible to develop a method for quantifying the secretory activity of the accessory glands, reduces the number of experiments and the consumption of insects required for DL5G to obtain reliable results, and also provides the possibility of expanding biochemical toxicological studies on isolated reproductive glands of insects, which is important when developing methods of insect control and methods of standardization of laboratory cultivation of them.
Ф о ) М у л а и 3 о б р е т е н и F o) M u l a and 3 o b e te n u
Среда дл экстракции полового секрета (13 придаточных желе: самцов комаров Aedes aegypti, содержаща хлористые соли щелочных металлов, отличающа с тем, что, с целью повышеин выхода полового секрета, в качестве солей щелочных металлов используют 1,27-1,29 М водный раствор NaCLSexual secretion extraction medium (13 adventitious jelly: male Aedes aegypti mosquitoes containing alkali metal chloride salts, characterized in that, in order to increase sexual secretion, 1.27-1.29 M aqueous solution is used as alkali metal salts NaCL
Источники информации, нрин тые во виимание при экспертизеSources of information used in the examination
1. Р. S. Chen Experientia, 1976, т. 32, № 5, р. 549.1. P. S. Chen Experientia, 1976, V. 32, No. 5, p. 549.
2.М. S. Kaulenas, R. L. Genobsky, Н. Е. Potwald, Q. L. Burns. «G. Expt. Zooi, 1975,2.M. S. Kaulenas, R. L. Genobsky, N. E. Potwald, Q. L. Burns. "G. Expt. Zooi, 1975
V. 193, p. 21.V. 193, p. 21.
3.M. S. Fuchs, G. B. Craig, E. A. Hiss, «Life science, 1968, v. 7, p. 835 (прототип).3.M. S. Fuchs, G. B. Craig, E. A. Hiss, "Life science, 1968, v. 7, p. 835 (prototype).
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792817823A SU843897A1 (en) | 1979-07-24 | 1979-07-24 | Medium for extraction of sex secretion from accessory glands of aedes aegypti mosquito males |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792817823A SU843897A1 (en) | 1979-07-24 | 1979-07-24 | Medium for extraction of sex secretion from accessory glands of aedes aegypti mosquito males |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU843897A1 true SU843897A1 (en) | 1981-07-07 |
Family
ID=20849849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU792817823A SU843897A1 (en) | 1979-07-24 | 1979-07-24 | Medium for extraction of sex secretion from accessory glands of aedes aegypti mosquito males |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU843897A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110191635A (en) * | 2017-01-22 | 2019-08-30 | 塞纳科有限公司 | For carrying out the method and device thereof of sex sorting to mosquito |
-
1979
- 1979-07-24 SU SU792817823A patent/SU843897A1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110191635A (en) * | 2017-01-22 | 2019-08-30 | 塞纳科有限公司 | For carrying out the method and device thereof of sex sorting to mosquito |
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