SU826244A1 - Method of quantitative determining of protein in samples - Google Patents
Method of quantitative determining of protein in samples Download PDFInfo
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- SU826244A1 SU826244A1 SU792790689A SU2790689A SU826244A1 SU 826244 A1 SU826244 A1 SU 826244A1 SU 792790689 A SU792790689 A SU 792790689A SU 2790689 A SU2790689 A SU 2790689A SU 826244 A1 SU826244 A1 SU 826244A1
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- USSR - Soviet Union
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- liver
- protein
- samples
- hepatoma
- tissue
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(54) СПОСОБ КОЛИЧЕСТВЕННОГО.ОПРЕДЕЛЕНИЯ БЕЛКА В ПРОБАХ(54) METHOD OF QUANTITATIVE. DEFINITION OF PROTEIN IN SAMPLES
медленно извлекают, перфузируют на льду дистиллированной водой, промокают бумажными фильтрами, тщательно очищают от соединительной ткани и взвешивают. Опухолевую тканб берут в логарифмической фазе роста, праймущественно с периферийных участков, исключа возможность, попадани в пробу некротической ткани. В опытах используют 33%-ный свежеприготовленный гомогенат (из расчета 1:2 веса на объем) . Концентрацию белка в ткани вы ражают в мг/г сырого веса.slowly removed, perfused on ice with distilled water, blotted with paper filters, thoroughly cleaned of connective tissue and weighed. Tumor tissues are taken in the logarithmic growth phase, primarily from the peripheral areas, excluding the possibility of necrotic tissue getting into the sample. In the experiments using a 33% freshly prepared homogenate (at the rate of 1: 2 weight per volume). The protein concentration in the tissue is expressed in mg / g wet weight.
Результаты опытов подвергают статической обработке методом Монцевичюте-Эрингене с использованием фактора Монденгауэра. Различи между средними арифметическими (М; расценивают как достоверные при значении Р ,05.The results of the experiments are subjected to static treatment by the method of Monzaviciute-Eringen using the Mondengauer factor. Differences between arithmetic averages (M; are regarded as reliable with the value of Р, 05.
Данные опытов представлены в таблце (концентраци белка в ткани печени регенерирующей, малигнизирующей, интактных животных и в ткани злокачественных гепатом).The experimental data are presented in the table (protein concentration in the liver tissue of regenerating, malignant, intact animals and in the tissue of malignant hepatomas).
10ten
Гепатома Г-27Hepatoma G-27
Печень ж.он. (Г-27)Liver w.on. (G-27)
Гепатома Г-22Hepatoma G-22
Печень ж.он. (Г-22)Liver w.on. (G-22)
Гепатома Г-6ОHepatoma G-6O
Печень ж.он. (Г-бО)Liver w.on. (G-B)
Гепатома Г-61Hepatoma G-61
Печень ж.он. (Г-61)Liver w.on. (G-61)
Гепатома Hepatoma
Печень ж.он. (Г-48)Liver w.on. (G-48)
Гепатома г-46Hepatoma g-46
Печень ж.он. (Г-46).Liver w.on. (G-46).
Печень интактного животного (крысы)Liver of an intact animal (rat)
10ten
6100,40±5,086100.40 ± 5.08
16И7,41±5,,00116I7.41 ± 5,, 001
12J,60±4,26 0,001 0,00112J, 60 ± 4.26 0.001 0.001
105,9р±5,,U5105.9 p ± 5,, U5
88,50±2,3488.50 ± 2.34
92,,6592, 65
Регенерирующа Regenerating
речень118Speech118
Печень ложнооперированных- животных 4Liver of the swiftly operated animals 4
животное-опухоленоситель; animal carrier;
.он, генеральное среднее;.on general average;
m - среднее квадратичное отклонение; m is the standard deviation;
I - веро тность различий генеральных средних дл опытной (гепатома и печень ж.он.) .и контрольной печени интактного животного групп биообъектов;I is the probability of general average differences for the experimental (hepatoma and liver) and control liver of an intact animal groups of bioobjects;
j веро тность различий генерального среднего дл опытной (гепатома ) и контрольной печени ж.он. групп биообъектов.j probability of differences between the general average for the experimental (hepatoma) and the control liver. groups of biological objects.
В таблице установлено высокодост .оверное увеличение содержани белка в ткани малигнизирующей и регенерирующей печени, а также в ткани всех исследованных злокачественным гепатом, кроме штамма .гепатомы Г-46, в сравнении с соответствующими контрольными ткан ми. Увеличенным также оказалось сод ержание белка в ткани печени животных-опухоленосителей со штаммами гепатом Г-22, Г-60 и Т-48. Эти результаты показывают, что повышенное содержание белка характерно дл тканей с усиленной клеточной пролиферацией как в норме (регенерирующа печень), так и при патологий (малигнизирующа печень, злокачественные renaTO2vffij) .The table establishes a high growth rate of a probable increase in the protein content in the tissue of the malignant and regenerating liver, as well as in the tissue of all investigated malignant hepatos, except for the strain H. 46 hepatoma, in comparison with the corresponding control tissues. The content of protein in the liver tissue of animals-tumor carriers with strains hepatitis G-22, G-60 and T-48 was also increased. These results show that an increased protein content is characteristic of tissues with enhanced cell proliferation, both in normal conditions (regenerating liver) and in pathologies (malignant liver, malignant renaTO2vffij).
Предлагаемый способ позвол ет существенно сократить врем анализа (на 2-3 ч), делает его менее трудоемким и более точным, а также обусловливает его эффективное применение в исследовании проб с повышенным содержанием липидов и полисахаридов.The proposed method allows to significantly reduce the analysis time (by 2-3 hours), makes it less time-consuming and more accurate, and also determines its effective use in the study of samples with a high content of lipids and polysaccharides.
Продолжение TaQjiHiJJjContinued TaQjiHiJJj
101,27±3,,001101.27 ± 3, 001
67,33±2,5267.33 ± 2.52
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792790689A SU826244A1 (en) | 1979-06-04 | 1979-06-04 | Method of quantitative determining of protein in samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792790689A SU826244A1 (en) | 1979-06-04 | 1979-06-04 | Method of quantitative determining of protein in samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU826244A1 true SU826244A1 (en) | 1981-04-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU792790689A SU826244A1 (en) | 1979-06-04 | 1979-06-04 | Method of quantitative determining of protein in samples |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU826244A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1983003673A1 (en) * | 1982-04-16 | 1983-10-27 | Ole-Christian Bjarno | Method for determining the content of collagen in a sample containing connective tissue |
-
1979
- 1979-06-04 SU SU792790689A patent/SU826244A1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1983003673A1 (en) * | 1982-04-16 | 1983-10-27 | Ole-Christian Bjarno | Method for determining the content of collagen in a sample containing connective tissue |
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