SU763333A1

SU763333A1 - Method of stabilizing cysteinehydrochloride

Info

Publication number: SU763333A1
Application number: SU782685461A
Authority: SU
Inventors: Эмилия Юлиановна Гудриниеце; Валентина Степановна Королькова; Зента Фрицевна Тетере; Дайна Роландовна Зицане; Раиса Михайловна Волынова
Original assignee: Рижский Ордена Трудового Красного Знамени Политехнический Институт; Латвийский Филиал Всесоюзного Научно-Исследовательского Института Комбикормовой Промышленности
Priority date: 1978-11-20
Filing date: 1978-11-20
Publication date: 1980-09-15

Description

(54) СПОСОБ СГАБИ.ПМЗАЦИИ СОЛЯНОКИСЛОГО ЦИСТЕИНА(54) METHOD OF AGABI. PLANTS OF SOLYNIC-ACID CYSTEIN

1one

Изобретение относитс к способу стабилизации сол нокислого цистеина, используемого в медицине, биологии и химии. Однако, ширсжому применению цистеина преп тствует его легка окисл емость на воздухе с образованием цистина.The invention relates to a method for stabilizing cysteine hydrochloride used in medicine, biology, and chemistry. However, the widespread use of cysteine is prevented by its easy oxidability in air to form cystine.

Известен способ стабилизации водных растворов цистеина, заключающийс в использовании многоатомных спиртов, таких как сорбит, в присутствии растворимых сульфитов, при рН среде 5,5 1.There is a method of stabilizing aqueous solutions of cysteine, which involves the use of polyhydric alcohols, such as sorbitol, in the presence of soluble sulfites, at a pH of 5.5-1.

Однако дл получени стабильных растворов цистеина необходим двухкрат1{ый избыток сорбита.However, in order to obtain stable solutions of cysteine, a twofold {2 th excess of sorbitol is necessary.

Известен также способ стабилизации водного раствора цистеина при использовании в качестве стабилизирующего агента моносахаридов (глюкозы, фруктозы) или дисахаридов (мальтозы , лактозы) 12.There is also known a method of stabilizing an aqueous solution of cysteine when using monosaccharides (glucose, fructose) or disaccharides (maltose, lactose) as a stabilizing agent 12.

В зтом случае получаетс устойчивый высогкоконцентрированный раствор цистеина, который пригоден дл инъекций в течение 60 дней. Полна стабилизаци раствора цистеина происходит только при большом избытке сахара,In this case, a stable, highly concentrated cysteine solution is obtained, which is suitable for injections for 60 days. Full stabilization of the cysteine solution occurs only with a large excess of sugar,

что не позвол ет получать растворы чистого иистеина.which prevents the preparation of pure iistine solutions.

Наиболее близким по технической сущности к описываемому вл етс способ стабилизации водных растворов цистеина с использованием в качестве добавок пирролидона или пироглутаминовой кислоты при рН 7,53 3.The closest in technical essence to the described is a method of stabilizing aqueous solutions of cysteine using pyrrolidone or pyroglutamic acid as an additive at pH 7.53 3.

Стабилизаци водных растворов цистеина по этому способу заключаетс в следующем.The stabilization of cysteine aqueous solutions by this method is as follows.

2 г хлоргидрата цистеина и 1,5 г пирролидона раствор ют в 100 мл дистиллирова1шой воды, подщелачивают бикарбонатом натри до рН 7,5 и получают раствор , устойчивый в течение 6 мес цев.2 g of cysteine hydrochloride and 1.5 g of pyrrolidone are dissolved in 100 ml of distilled water, alkalinized with sodium bicarbonate to pH 7.5 and a solution is obtained which is stable for 6 months.

Недостатки зтого способа состо т в том, что. при. стабилизации вводитс значительное количество пирролидона, что не позвол ет получить растворы чистого цистеина, способ позвол ет стабилизировать цистеин только в растворах , а также сравнительно непродолжительна устойчивость стабилизированного цистеина.The disadvantages of this method are that. at. stabilization, a significant amount of pyrrolidone is introduced, which does not allow obtaining solutions of pure cysteine, the method allows stabilization of cysteine only in solutions, as well as the relatively short stability of stabilized cysteine.

Claims

The purpose of the invention is to increase the stability of cysteine hydrochloride and reduce the content of impurities in it. 37 This goal is achieved by using 2.2-methylene-di- (4-methyl-6-tert-butylphenol) NG-2246 as a stabilizing additive in an amount of 0.005 0.014 by weight of cysteine hydrochloride. The stabilizer is preferably added during the crystallization of cysteine hydrochloride. The essence of the method is as follows. To a saturated solution of cysteine hydrochloride, ethanolic solution NG-2246 is added in an amount of 0.005-0.01% by weight of cysteine and left for 24 hours at 0 ° C. The crystals are filtered out, formed and stored under normal conditions. When storing cysteine sulfate, stabilized with 0.005-0.01% NG-2246 at room temperature for a year, even traces of cysteine are not detected, while in the control sample the amount of cysteine for this period reaches 37.5%. Example 1. The south of cysteine hydrochloride is dissolved in 17 ml of dilute hydrochloric acid (1: 1) and 0.01% by weight of cysteine NG-2246 dissolved in ethanol is added and left at 0 ° C for 24 hours. The precipitated crystals are filtered off and dried, and 9.5 stabilized hydrochloric cysteine is obtained with so-called pcs. 178-180 ° (decomp. 172-180 °. Found,%: Sat 21.52 N 8.20 S 19.61 times; Calculated,%: Ce21.78 N 8.30 S 19.86 The resulting product is left at room temperature temperature during the year. Example 2. The south of cysteine hydrochloride is dissolved in 17 ml of dilute hydrochloric acid (1: 1) and 0.02% by weight of cysteine NG-2246 dissolved in ethanol is added and left for 24 hours at 0 ° C. The precipitated crystals are filtered off, and 9.4 g of stabilized hydrochloric cysteine are obtained, with a fluent phase point of 178-180 ° (diff.) Found:% ce 21.49, N 8.15 S 19.56 Calculated,%: Cg 21.78 N 8.30 S 19.86 The resulting product is left at room temperature temperature during the year. The proposed method for stabilizing cysteine hydrochloride is very simple and adaptable. And it can be used in the synthesis of cysteine, since the stabilizer HG-2246 is introduced into the crystallization process, without requiring additional operations. additives, characterized in that, in order to increase the stability of the target product, 2,2-methylene-di- (4-methyl-6-tert-butsh1phenol) in an amount of 0,, 01% by weight cysteine sulfate. Sources of information taken into account in the examination 1. Japanese Patent No. 31993, RZHH 17N200P, 1972.

2. The patent of Japan G 15597, RZHH 10N553P, 1968.

3. Japanese patent number 31994, RZHH 17N20Sh, 1972 (prototype).