SU745932A1 - Method of biomass production - Google Patents

Description

This invention relates to a microbiological PE), and a method for producing baker's yeast biomass.

There is a known method of growing yeast in a magnetic field, which implies that the intensification of yeast growth is achieved by cultivating yeast at the south pole of a permanent magnet with a capacity of 2400-2600 O. UllA well known method of expressing negative air ions on them 2.

However, this method provides for the processing of culture. To produce a superficial method, which leads to a change in morphology (cell size), and not an increase

biomass.

A known method for producing biomass by cultivating baker's yeast on a nutrient medium containing a source of carbon, nitrogen, phosphorus, a mineral salt, growth stimulator and trace elements during aeration of the medium, followed by maturation and enhancement of the target product. The essence of this method

is that the intensification of the process of growing yeast is achieved by increasing the volume through the medium. MOB finely dispersed air. The amount of it is increased 4-15 times as compared with the oxygen of the air necessary for the growth of yeast 3.

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The drawbacks of the method are a slight intensification of the yeast multiplication process, the production of yeast cells with insufficient volutin content and activity of some enzymes of the tricarboxylic acid cycle, the supply of large volumes of finely dispersed air, which requires large capital investments to replace air distribution systems, yeast plants and blowers.

The purpose of the invention is to intensify the process of biomass accumulation, increase the content of volutin in the cell, activate some enzymes of the tricarboxylic acid cycle and reduce the consumption of air required in the yeast growth apparatus. The set goal is achieved by tf ligisiece K5 lithium aeration of the medium is carried out with air containing negatively charged ions with a concentration of 0, 5-lOcM-, while the supply of negatively charged ions during aeration with air takes place for 5–20 min. start frequency: 1a process kul1 t y1111r 5 vani. RA: reduction of air required for aeration is reduced by 15–20%. . For transportation of negative charged air ions, I use MeMvDiesz in the company of Aerosystem and Air Blower. For the formation of negative and new air, the following processes are carried out: 1. Capture of electrofusion by a Hyyy molecule with emission of an quantum of energy A + e hV 2. Electron capture of a molecule with subsequent dissociation of AB + e A + B 3. Formation of negative ion in case of electrolysis of a molecule to ions by electron impact without the capture of an impacting electron AB + e e. L ............. i. The formation of negative air ions proceeds as follows: + e O + + O2 Oe + cpi - co + o CO2 + .O- CO + Og. The proposed method is characterized by the intensification of a procession, a higher content of volutin in the cell, more active enzymes of the tricarboxylic acid cycle, an economical air flow, nppcTOTOjB, does not require additional capital investments, because the equipment used for the implementation is available on the enterprise. It is known that the basis of respiration is redox a; i «« t г; ьie ....-..- v -,.-..- „j,. ,,., - .. reactions. During the oxidation of organic substances, the cell releases energy, which is necessary for its healthy life. The presence of negatively charged air ions increases the oxidative oxygen potential of the cell and itself activates the cell viability. The activity of the enzymes of the tricarboxylic acid cycle, for example, alcohol dehydrogenase, malate dehydrogenase and glutamate dehydrate, prerogatives increase and the respiratory capacity of yeast cells increases. Intensification of respiration leads to an increased metabolism of the cell, to a more active accumulation of yuutin, which also contributes to active reproduction. Negatively, charged ions can also change a number of biological and biochemical properties in biological objects (such as cell membrane permeability and enzymatic activity) i by transferring electrical charges to the object and settling on the surface of the HFHH substance. Integration of the biomass accumulation process during aeration of the culture medium with air containing negatively charged ions at a concentration of 0.5, 5–10 cm is also explained by an increase in the permeability of cell membranes, causing a more intense influx of nutrients into the market. The ability of yeast to multiply after exposure to negative ions of air increases 1.5-2.0 times, the economic utilization rate of the raw materials increases by 5-10%. The content of volutin in the cell increases by 2-3 times, the activity of enzymes, such as alcohol dehydrogenase, malate dehydide. of rogenase and glutamate dehydrogenase is increased by 10-15%, and the lifting power of yeast is improved by 20-30% compared to known methods., Example. The stock or commercial yeast is grown by one of the airborne or air flow methods accepted by the yeast industry, using the equipment available at the plant. In addition, an ion generator (ionizer) is installed, as well as a high voltage source, ammeters and voltmeters. The ion generator is connected to the duct supply. air in .growing machines-. Nutrients are prepared in a known manner. Yeast is grown in an apparatus with a total capacity of 100 m according to the air-flow method at a ratio of dilution of raw material KR-12 and a temperature of 30-33 ° C, controlled by a special device, and the supply of nutrients and water. The culture medium is aerated with a breath containing negatively charged ions with a concentration of 2, while the supply of negatively charged ions during aeration of the culture medium with air is carried out periodically. Negative ions serve

Every hour from the beginning of the cultivation process for 15 minutes. The air flow required for aerating the environment is reduced by.

At the end of the process, the yeast is ripened in the usual way, separated, washed / filtered and stored in yeast milk collections or in refrigerators.

The proposed method can be used in the cultivation of both uterine and commodity yeast, as well as in the processing of yeast milk of uterine yeast after growing them or before sowing into a commodity yeast-growing apparatus.

The method of obtaining biomass by cultivating baker's yeast on a nutrient medium containing a SOURCE of carbon, nitrogen, phosphorus, mineral salts, growth promoter and trace elements during aeration of the medium, followed by ripening

and isolating the target product, which is characterized by the fact that, in order to increase the biomass yield with an increased Content of LuTin in the cells, activation of the enzymes of the tricarboxylic acid cycle, decrease of the air consumption, thereby intensifying the process, the aeration of the medium is negatively charged during the cultivation process. 0 to 10–10 cm / at the same time the supply of negatively charged ions in the process of aeration with air that is injected for about 5–20 minutes every hour from the beginning of the process of cultivation and.

Sources of information taken into account in the examination

1. The author's certificate of the USSR 374369, cl. C 12 C 11/00 opUblik.

0 1971.

2. Patent Sapa 3133003, cl. 195-81, publ. 1964.

3. The patent of Switzerland No. 508721, cl. C 12 C 11/08, published 1971.

Claims (1)

Claim A method of producing biomass by cultivating baker's yeast on a nutrient medium containing a source of carbon, nitrogen, phosphorus, mineral salts, a growth stimulator and trace elements during aeration of the medium, followed by maturation and isolation of the target product, characterized in that, in order to increase the yield of biomass with increased The content of Vojutin in the cells, activation of enzymes of the tricarboxylic acid cycle, reduction of air consumption, and thereby intensification of the process, aeration of the medium is carried out during cultivation hamster containing negatively charged iony a concentration of 0.5 × 10 ⁵ -2.5 × 10 ⁹ cm / ⁵ wherein the flow of negatively charged ions in the process carried out aeration air peryodycheski ^ a 'for 5-20 minutes every hour from the beginning of the process cultivation.