SU731976A1 - Method for improving fertility of small rodents - Google Patents

Description

one

The invention relates to a technique for growing laboratory animals (rodents) and can be applied in a national household.

Medicine and agricultural practice have long used the beneficial effects on the body of various physical factors, in particular, irradiation with infrared and ultraviolet rays, x-rays and gamma rays.

On the stimulating effect of electret magnetic publications on the organism of higher animals of single-celled organisms, there are separate indications in the literature, in particular, in the works of R. I. Petrova S, A. A. Pressman, and others.

Although these works generally agree with the teachings of I. M. Sechenov, I. P. Pavlov, and L. A. on the positive trophic effect of weak stimuli on the body, however, none of these works contain

Specific data on the quantity of doses of electromagnetic radiation, leading to the achievement of an optimal effect on the fertility of living organisms.

Along with these works, there are also other data that tell us about the absence of the stimulating effect of electromagnetic fields on living organisms (M. S. Tolgska, 3. V. Gordon. V. T. 4, and others.

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The known methods for increasing fertility are that animals, for example, fur-bearing animals (minks),

j irradiated with ultraviolet energy with the aid of the PRK-2 lamp at a dose of 36OO the mayor. hour / m 5. With such irradiation, a small (by -1.5%) increase in the number of parents of females is achieved, and the pups yield to the regular female reached 4.2 compared to 3, 7 in non-irradiated animals. However, some time after the receipt of the product, the number of survivors per standard CQMfO

becomes almost equal to that of unirradiated animals. Thus, until now, the question of the possibility of stimulating the fertility of living organisms, in particular rodents, with the help of electromagnetic radiation is debatable, and the information contained in the literature against Y} is curative. The authors are not aware of any experimental work on the basis of which to judge the stimulating fecundity of mammal mammals, the action of the microwave drink.

The purpose of the invention is to increase the efficiency, stimulate the reproduction of small animals - rodents.

This goal is achieved by the fact that females that have reached puberty are affected by microwave radiation of the decimeter range with a surface power flux density within the subthermal levels of 0.1-1O, O mW / cm with a duration of 2O-50 minutes, and the sessions are repeated once every two days, irradiation sessions begin 1.5–2 months before mating with unirradiated males, and 5–6 sessions are performed after the onset of pregnancy.

Example 1 The experiment was carried out in sexually mature white outbred mice of 2-2.5 months of age with an initial weight of 20-22 g. A radiation-generating apparatus was taken at the source that generates microwave radiation at a frequency of 2459 MHz {wavelength 12.4 cm |. In the experiment, there are 50 female mice; 60 mice mice served as controls (non-irradiated animals). The animals of the experimental group were subjected to microwave irradiation at a super bossed power flux density of 2.5 mW / cm - and an irradiation duration of 47 minutes per session. Irradiation sessions were performed once every two days. After 26 irradiation sessions, the females were mated with unirradiated males (3: 1 ratio) and after that another 5 irradiation sessions were carried out (the total number of sessions was 31). After each irradiation termination, each female was deposited in a separate cage and the fertility and physical development of offspring.

As a result of this experiment, a statistically significant (with REO, 95) increase in the number of parents was obtained & females (by 16%), an increase in the ratio of the number of born animals to the number of full-time females (by 0, 83 or 21%), and an increase in the ratio of the number of animals that died to the age of six months to the number of full-time females (by 0.7 or 2O%), At the same time, the average body weight of animals of month age did not have significant differences compared with the control.

Example 2. Irradiation of white female mice was performed at a surface power flux density of 5 mW / s and an irradiation session duration of 22.5 minutes per session. In experience, there are 36 female mice; 49 female mice served as controls. All other experimental conditions are similar to those of Example 1,

As a result of this experiment, a statistically significant increase (at, 95) in the ratio of the number of births to the number of regular females (by 1.2 or 33%), as well as the increase in the ratio of the number of live to the monthly age of animals to the number of established females (by 0 , 7 or 22%). At the same time, the average body weight of animals of the month of age who were born from female females was substantial (with, 95 exceeded body weight in the control (by 1.4 g or 15%).

The main advantage of the present invention over existing proposals is the high efficiency of the proposed method for increasing the fertility of small rodent animals, in particular laboratory animals, expressing in obtaining a significant (up to 33%) increase in the basic fertility indicators of animals while maintaining a sufficiently high viability of the offspring.

Based on the results of experimental studies, it is already possible to make an approximate calculation, the refinement of which can be obtained with reference to a certain animal species. As an initial maximum value, the total surface energy density of radiation needed to stimulate reproduction in animals that have larger body sizes than in mice should be taken equal to (ZBOOR-j, and the lower limit is determined by the minimum positive effect of irradiation observed). and When the total tail energy density of the radiation energy is O, 01 Wh / cm (3 € D; W / cm). The application of the invention in nurseries for breeding and raising small labrator animals-rodents (white mice, white). The rats, guinea pigs, etc.) will significantly increase the offspring of these animals. The invention can also be applied to increase the fertility of small commercial animals-rodents. To evaluate the efficiency of implementation of the proposed method of increasing the fertility of small rodents in the national economy, its technical and economic substantiation is necessary. the use of a beam-58 apparatus with a 150-watt output power as a source of microwave radiation creates a fairly uniform (within tlO%) radiation field of 900 volts this field, depending minute size of the animal, can be placed two (rat or several smaller Zh1shotnyh. If the specified device is equipped with additional radiators, the productivity of the device will increase 10-15 times. The passport life of a single magnetron (the main limiting element of the microwave generator) is about 4OO hours of continuous operation, and its retail value is 6 O rubles. Under these conditions, the cost of the entire irradiation cycle of one animal (taking into account the depreciation of the apparatus and the energy consumed) will not exceed 30 kopecks, which is an negligible share of the cost of maintaining the animal. In the case of mass (industrial implementation of the proposed method, the CTOifMOCTb radiation exposure is reduced several times by the national economy. Formula of the Invention A method for increasing the fecundity of small rodents, mainly mice, by acting on them with electromagnetic radiation, characterized in females that have reached puberty are exposed to microwave radiation of the decimeter range with a surface power flux density within subthermal levels of 0.1-10.0 mW / cm sessions lasting minutes, the sessions are repeated once every two days, and the irradiation sessions begin 1.5-2 months before mating with non-irradiated males, and 5-6 sessions are performed after the onset of pregnancy. Sources of information taken into account during examination 1 Petrov R.I. The effect of microwave radiation on the human body and animals, L., Medicine, 1970, 71-73; 99-101. 2. Pressman A. A. Electromagnetic sex and nature is alive. M., 1968. 3 M. Tolgska, Gordon 3. V. Morphological and physiological changes in the body when exposed to electromagnetic waves of radio frequencies. M., Medicine, 1971. 4. N. N. V. Clinical Aspects of Microwave Radiation. P., Medicine, 1971. 5. Report on the finished topic No. 10 The effect of ultraviolet radiation of minks on fecundity of females and development of offspring. M., 1973. The Order of Lenin Agricultural Academy named. KA Timir throat.

Claims (1)

Claim A method of increasing the fecundity of small rodents, mainly mice, by exposing them to electromagnetic radiation, characterized in that, in order to increase efficiency, females that have reached puberty are exposed to decimeter-wave microwave radiation with a surface power flux density within subthermal levels of 0.1 —10.0 mW / cm with sessions lasting 20-50 minutes, repeat the sessions once every two days, and the irradiation sessions begin 1.5-2 months before mating with unirradiated males, and 5-6 sessions dressed after pregnancy.