SU721103A1 - Interferon inducer - Google Patents

Description

(54) INTERFERON INDUCTOR

This invention relates to the field of medicine, namely virology. . Synthetic polynucleoids and viruses 1 are known interferon inducers. However, these preparations are expensive and have antigenic activity. Co-CncSnc-Hd It The proposed compound is known as an immunosuppressor 2. The aim of the invention is to expand the arsenal of inductively. This goal is achieved by the use of the gossypol sous ethyl sulfate Nstry of the formula OHH (-} g: -Hg-s Hg Oz a O

721103

-.- v v-u: -; - I

Interferon Induction GSP in Chicken Embryo Fibrobdust Cultures

Experimental conditions

Control 31 μg / ml gene 62 MCH / Ш1 gene 125 μg / ml gene 250 μg / ml rtH

The preparation of interferon, obtained by treating cells with a 250 μg / ml gene, heated at 5ba for 1 h

The preparation of interferon, obtained by treating cells with 250 μg / ml of HCH, exposed to trypsin

1-10 μg / ml poly IC in the presence of 70-100 μg / ml DEAE-dextran

Eindbis virus

2. To determine the antiviral activity of a gene in a cell culture, take 2-3 cc exact cultures of embryonic fibroblasts of chickens and spread the virus vesicular hundred jjajTHT) with a multiple biological rate of Then BATH per cell. After a 30-minute adsorption at 3-7e, the virus containing the liquid is sucked off, the cells are washed off with Hank's solution, and the fresh nutrient medium containing the preparation is washed out. After 24 h, when in the control the expression is observed; The cytopathic effect of the virus is removed, the fluid is discharged, and the virus is determined by a titration method under the surface of an undercoat. So, if the virus titer in the control reaches 9.2 BbE / m in the treated 120 µg / ml gene, the virus production cultures are 5.0-tg PFU / MP, the degree is suppressed {I: the virus multiplication exceeds 4 pfu / ml t. e. products, the virus is reduced 10,000 times. .......-Etc . imper 3. Fluid serum interferon is prepared by injecting mice with a 500 µg / mouse yntraperitoneal gene. The preparation was administered 2 times with the interval of 24 hours. Kontsebledystilirovanny water was administered to the controversial mice. After 24 hours and 48 hours after the last injection of the drug from, under the clavicular, the arteries take blood, serum is obtained. Interfering with this method is the interruption of the virus test (BBC) on mouse cells .q. In the absence of viral inhibitors, the activity of interferon controls in BFU5OuGd, d

10 210 80 640

640

ten

64-128 512 320 potent serum interferon titers with a dose of 500 µg / mouse of the preparation are after 24 hours 320 over 48 hours 160 HEso / Ai / t Example 4. Antiviral activity of the gene in experimental animals (mice 16-18 g) is determined by administering (intraperitoneal o) 500 µg / mouse (25 mg / kg). After 24 hours, the same dose of the gene was reintroduced, and immediately after that, 1,000 to 10,000 PFU of eindbis infected the mice intratrarebrally in a volume of 0.03 ml. 24 hours after the infection, the mice are sacrificed, the brain is removed and a 10% brain suspension is prepared, which is titrated by the plaque method under an agar coating on chicken embryo fibroblasts. Example 5. About Exactly Gene. Different concentrations of the gene are added to the chicken fibroblast cell culture and the cytotoxic dose of the preparation is determined. In animal experiments, mice (16–18 g) were administered intraperitoneally different doses of the drug and monitored for 6 days. At a concentration of up to 2500 µg / mouse (125 µg / kg), the gene has no visible toxic effect. The interferon inducer allows you to increase interferon titers and has a wide spectrum of antiviral. Claims of the invention. Use of gossypol-α-aminoethyl oxide of sodium oxide formula

5721103

KOOj-CH2 3H2HH-H J NOH HYG-KH-CHG-CHG OzNa as an interferon inducer. Sources of information, 10 / trinty are taken into account when soyvtiz 14YgLer Sh13p1y 1g№1, On 1'-Noeapa.t81er (Zat. 2. Copyright x; USSR 545634, k4C07C87 / 28, AF1k31 / 35.1974.

Claims (1)

Formula! inventions The use of gossypol - / - sodium aminoethyl sulfate of the formula 5 721103 6 COO, - ^ I _r Sn _r NN ~ AND (} 0H Oh Well> -nn-y1 _g CH _r 0ena ° t ^L t / \ A ^ 0 G fl I but ^ssS ch _s n ₅ s L ii j F> F ^ he is he Chj Chj C * as an inducer of interferon.