SU703758A1 - Apparatus for manometric determining of gas exchange9 - Google Patents

Description

(54) APPARATUS FOR MANOMETRIC DEFINITIONS

GAS EXCHANGE

The invention relates to devices designed to study the gas exchange of biological objects, mainly in microbiology, biochemistry and plant physiology, and can also be used to study the physicochemical processes that occur with the release or absorption of gas. A device for determining gas exchange is known, comprising a 1 / -shaped manometer and a reaction vessel fl. The apparatus does not allow the gas exchange to be studied in dynamics, since the gas emitted or consumed is measured by supplying the corresponding gas to the reaction vessel or removing the corresponding gas from it until the previous level is restored - the pressure recorded by the pressure gauge to which the reaction vessel connects through the gaseous medium. Known apparatus consisting of a thermostat, fortified on it 14-16 U-vol. different pressure gauges having a tap in meo. The bending and reaction vessels attached at the time of exposure and pressure measurement to the corresponding pressure gauges. One hand, the pressure gauge is open and communicates with the atmosphere, and the second is equipped with a two-way valve, in front of which is connected the pressure gauge to the pressure gauge 2. the pressure valve is closed, which ensures the tightness of the container, which is not sealed outside the connection with the pressure gauge. At the same time, the gas atmosphere of the tank itself. communicates with the gas medium of the manometer located above the manometric liquid, and the constancy of the volume is ensured by bringing the level of the manometric liquid in the knee of the manometer with which the container is connected, always to the same zero position. However, the well-known apparatus does not have sufficient performance, which is not sufficient for complying with mass analyzes that require for a continuous exposure.

Dech y "gagak;

  7O3758 since the capacitance during the whole: aJ -j: is A.its "J $ itsi J $ iS -SiS 1 v-; --i. -r. ----.-.---: - i -, .-; - j;, -.-: U ..-;.: ..-.-- C. ;. - -.--: ..-. -. the observation time, the EK BONUS exposure period and the time taken to measure the pressure are connected to the corresponding Manometer. At the same time, the exposure time significantly exceeds the time of pressure measurements, thus, the analysis time spent is quite large. - - x ... The purpose of the present invention is to increase the productivity of the apparatus C, the accuracy of the analysis by stabilizing the temperature of the gauge fluid. This goal is achieved by the fact that the regenerative tank is equipped with a MaoMetric fluid vessel installed inside it with a MayoMetric fluid vessel, in which the connecting tube is placed, and the pressure gauge is connected to a thermostat. FIG. Fig. 1 is a general view of ailnapa a, Fig. 2 shows a reaction vessel with a vessel, and a connecting unit of a manometer, forming a liquid shutter. The device consists of a manometer representing a U - shaped tube 1, placed together with it. with a scale of 2 in a profiled thermostatically-controlled casing 3, with the outlet for 4 which is common to both knees of the mani- ter 4 facing upwards. One of the kOlen meters of the ma- meter, equipped with a d & ampholet tap 5, ends with a connecting tube or a hollow needle 6 and is filled with a manometric locking fluid 7. The second bend of the manombra with a hose 8 having a two-way cock 9 is immersed at a constant depth into the manometric fluid 10 which is located in the vessel 11, which communicates with the atmosphere, To the outlet 4 by means of a vacuum junction 12, equipped with a two-way edge 13, when the piston (syringe) 14 is connected, the liquor 7 fills the right-hand knee of the pressure gauge b ki (needles) to conditional zero point 15. The reaction container 16 germefyziro bath elastic stopper with uplotnitolnym cap 17 which has an opening for svobodyogo Yroh zhShy - 1№l1 1 (connection tube). If necessary, a container with a jockbone process is used, —Vyutri containers, except for a glass cup of 18 bg, an absorber of gaseous substances (for example, carbon dioxide) is equipped with a vessel 19 with a manometric locking device.

4 LIQUID 20. Thermostatic control of the vessel capacity and pressure gauge is provided by a thermostat 21. The device operates as follows. The necessary ingredients of the incubation mixture are introduced into the working space of the reaction vessel 16, and the vessel 19 is filled with a manometric (locking) liquid (for example, Brodie's liquid). The volume of the manymetric fluid should not exceed half the volume of the vessel 19 and at the same time should be sufficient for one knee of the manometer. The container is sealed with an elastic stopper and a sealing cap 17. The preparation of the mayometer is reduced to filling the right knee with a locking (gauge) fluid. To do this, open the edges 5 and 13, close the valve 9 and with noMoiffbto, the piston (syringe) 14 pump the locking fluid to the conditional zero point 15. The valve 5 closes. The reaction vessels are thermostatted and, if necessary, shaken. One Thermostatic Pressure Gauge. At the same time; The temperature of the lock-up fluid in the manometer is equalized with the temperature of the shut-down fluid in vessel 1, thereby increasing the measurement accuracy. After equalizing the temperature, the reaction tank is connected to a pressure gauge and the pressure gauge pressure tank is measured. For this, a hollow needle (connecting tube) of the manometer with Aykrytny tap 5 is inserted through an elastic stopper with a sealing cap 17 into the vessel 19. With a gray bore 20. I open the crane 5 and 9 and with the help of syringe 14 install the M0NISK locking (manometric) liquids in the right knee of the manometer at zero mark 15; The value of pressure in the tank is judged by the level of the manometric fluid stand in the left knee (in mm gauge fluid). The valve 5 is closed and the container is disconnected from the MaHOMetpa yut of removing the needle 6 from it. After the eigo, the manometer is very hot and comfortable to measure the pressure in the next reaction vessel. The calculation of the number of the readings or by the single Gaza is made on the basis of the main pressure gauge, taking into account nbiipaiskH per thermobarometer. Con-

5703758

The stant of the vessel is calculated using a crane shaped on one of its knees,

Barcroft-Var urga. Distinguished by the fact that, with

The proposed apparatus allows for an increase in the production of water

, To carry out mass analyzes that require length and increase accuracy by careful observation, it is difficult to calibrate the manometric temperature.

Birtb with the help of the famous eb1vf & t &. Prigidnosti, the reaction tank is equipped with

In this way, its performance is compared to the setting inside the box.

with a capacity of izvestnoi1CH} SHMary-vessel with W liquid,

it increases in proportion to the term on the axis of which is kyugo; raembschev a is connected

dishes .10 tube,

to / 2. The apparatus according to P.1, 6 tl and h and y

Claims (1)

The claims of the meter are supplied with the thermostatically controlled skin AnnJapaT for a manometric determination. 5 Sources of information of gas exchange, including a thermostat, taken into account in the examination of the reaction capacity placed in it1. England patent number 1377О44 and connected to it with the help of connect-iuuG 1 V, 1974, The tube is a U-shaped manometer2. KN, Gavrgoenko V, F. Large practical practice filled with manometric liquid of the godfathers on plant physiology, M ,, Higher and equipped with a diversion tube and, mouth-school, pp. 51-63, 1975, m and with the fact that the U-shaped mano, xom connected to a thermostat,