SU701641A1 - Method of the preparation of peptide - Google Patents
Method of the preparation of peptideInfo
- Publication number
- SU701641A1 SU701641A1 SU782638259A SU2638259A SU701641A1 SU 701641 A1 SU701641 A1 SU 701641A1 SU 782638259 A SU782638259 A SU 782638259A SU 2638259 A SU2638259 A SU 2638259A SU 701641 A1 SU701641 A1 SU 701641A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- peptide
- hydrolysis
- carried out
- specificity
- preparation
- Prior art date
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- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Description
(54) СПОСОБ ПОЛУЧЕНИЯ ПЕПТИДА(54) METHOD OF OBTAINING PEPTIDE
II
Изобретение относитс к вакцинно-сывороточному производству и касаетс получени пептида дл создани монорец,елторнь1х сывороток и специфических диагностикумов.The invention relates to vaccine serum production and relates to the preparation of a peptide for the production of monoecine, alternative serums and specific diagnostic kits.
Известен способ получени пептида путем гидролиза флагелина трипсином с последующим хроматографическим разделением, элюцией и диализом целевого продукта 1.A method of producing a peptide by hydrolysis of flageline with trypsin is known, followed by chromatographic separation, elution and dialysis of the desired product 1.
Однако длительный гидролиз снижает активность и специфичность пептида.However, prolonged hydrolysis reduces the activity and specificity of the peptide.
С целью повышени активности и специфичности пептида по предлагаемому способу гидролиз ведут в течение 20-30 мин и злюируют целевой продукт смесью, содержащей 80- 120 мл муравьиной кислоты, 250-350 мл уксусной кислоты и 530-670 мл воды.In order to increase the activity and specificity of the peptide according to the proposed method, hydrolysis is carried out for 20-30 minutes and the target product is zoned with a mixture containing 80-120 ml of formic acid, 250-350 ml of acetic acid and 530-670 ml of water.
Предлагаемый способ осуществл ют следующим образом. Мономерную форму Н-антигенов d, а, в, 1, 2 получают путем прогревани ее полимерной формы при 100 С в течение 1 мин. К полученному мономеру прибавл ют трипсин в соотнощении 1/100. Гидролиз провод т в 0,02М аминоацетатном буфере при рН 8,2. Гидролиз ведут в течение 20The proposed method is carried out as follows. The monomeric form of the H-antigens d, a, b, 1, 2 is obtained by heating its polymer form at 100 ° C for 1 minute. Trypsin is added to the monomer obtained in a ratio of 1/100. The hydrolysis is carried out in a 0.02 M amino acetate buffer at a pH of 8.2. Hydrolysis lead for 20
мин. Полученный гидролизат нанос т на колонку с сефадексом Г-200. Элюцию провод т НСООН : СНзСООН : HjO в соотношении соответственно 1:3:6. Препарат 1 пика диализуют и лиофилизируют. Изуче1ше серологической активности в РТПГА или радиоконкурентным методом показало, что препарат 1 пика всегда обладает серологической активностью, более высокой, чем исходный препарат флагелина.min The resulting hydrolyzate was applied to a Sephadex G-200 column. The elution is carried out by UNSL: CH3C: HjO in a ratio of 1: 3: 6, respectively. Drug 1 peak is dialyzed and lyophilized. A study of serological activity in RTPGA or radiocompetitive method showed that the drug of peak 1 always has a serological activity that is higher than the original drug flageline.
Флагелин несет на своей поверхности как специфическую антигенную детерминанту, гак и общую дл р да Н- антигенов (титр сыворот .ки с диагностикумом к H-d 1 : 16000, титр . сыворотки с диагностикумом к Н-а 1 : 4000). Полученный пептид несет на своей поверхности только специфическую антигенную детерминанту (титр сьшоротки с диагностикумом к H-d 1 : 32000).Flagetelin bears on its surface as a specific antigenic determinant, a hook and common for a number of H-antigens (serum titer with diagnostic to H-d 1: 16000, titer. Serum with diagnosticum to H-a 1: 4000). The resulting peptide carries on its surface only a specific antigenic determinant (titer with a short-term diagnostic to H-d 1: 32000).
Значительное сокращение времени ферме тативного гидролиза и применение указанной смеси растворителей создают услови , noBbimi ющие активность и специфичность пептида.A significant reduction in the time of fermentative hydrolysis and the use of this mixture of solvents create conditions that noBbimi the activity and specificity of the peptide.
. .
701641701641
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782638259A SU701641A1 (en) | 1978-05-11 | 1978-05-11 | Method of the preparation of peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782638259A SU701641A1 (en) | 1978-05-11 | 1978-05-11 | Method of the preparation of peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU701641A1 true SU701641A1 (en) | 1979-12-05 |
Family
ID=20774262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU782638259A SU701641A1 (en) | 1978-05-11 | 1978-05-11 | Method of the preparation of peptide |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU701641A1 (en) |
-
1978
- 1978-05-11 SU SU782638259A patent/SU701641A1/en active
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