SU699796A1 - Method of obtaining biostimulant of fodder yeast growth - Google Patents

Abstract

The CRAFT FOR PREPARING THE GROWTH GROWTH BIOSTIMULATOR, which involves hydrolysis of peat with mineral acid, neutralization of hydrolysis followed by separation of the solid residue, characterized in that, in order to increase the biological activity of the finished product, before the separation of the solid residue, the hydrolyzate is treated with sodium hydroxide and then sodium hydroxide is treated with sodium hydroxide to which the organic residue can be used. and hold at 38-40 ° C for 50-60 minutes, and the solution obtained after separation of the solid residue is neutralized and evaporated to a solids content of 45-50%. (L

Description

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1 (; About QD

The invention relates to the microbial industry, in particular, to a method for producing a biostimulating additive from peat when protein feed protein yeast is added. A known method of producing growth stoller is to hydrolyze peat with diluted mineral acids, neutralizing the hydrolyzate and separating the solid residue. However, the existing method of obtaining a biostimulating additive allows to obtain a product with relatively low biological activity, which increases the biomass yield of yeast by no more than 20%. In order to increase the biological activity of the biostimulant from peat in a known method, before separating the solid residue, the hydrolyzate is treated with sodium hydroxide to a pH of 12-13 and kept at 38-40 ° C for 50-60 minutes, and the solution obtained after separation of the solid residue is neutralized and evaporated to a dry matter content of 45-50%. As a result of operations in the proposed sequence and under the specified modes, additional destruction of the organic matter of peat takes place, the carbohydrate-containing product is enriched with humic acids, fulvic acids, amino acids, organic acids, i.e. compounds having a biostimulating effect on the yeast cell. Moreover, this biostimulating action is significantly more effective than the separate introduction, for example, of humic acids and peat hydrolysates into a nutrient medium. Example 1. Sedge peat with a degree of decomposition of 20–25%, a net of 28–30% is loaded into a hydrolayer reactor, and a solution of 2% hydrochloric acid is poured. The ratio of solid and liquid phases is 1:20. The contents of the solution are boiled for 4 hours with stirring to convert monosaccharides and partially the amino acids of organic and uronic acids, as well as related VSM compounds, into solution. The suspension is cooled to 38-40 ° C and at this temperature sodium hydroxide is introduced into the reactor in the form of a 10% aqueous solution to a pH of medium 12-13. In the hydrolyzate alkaline solution, the residue of peat at the indicated temperature is held for 50–60 min with stirring. Then, the liquid phase is separated from the solid by filtration, neutralized with hydrochloric acid to pH 6-6.5 and diluted to a dry matter content of 45-50%. It is essential to maintain the temperature (38-40 ° C) during the alkaline treatment of peat. Thus, with an increase in the temperature above 40 ° C, a monitor is observed with a significant decrease in the content of monosaccharides due to their decomposition in an alkaline medium. Similar phenomena reappear with an increase in the alkaline peat treatment time, as well as with an increase in the pH of the alkaline suspension medium. With a decrease in temperature, pH of the medium, and a decrease in the processing time, alkaline soluble substances are not fully recovered. The composition of the acid-base peat processing product contains the following components, wt.% Per organic mass: Reducing substances 0.1-1.2 Uronic acids Monosaccharide Humic acids Fulvic acids 3.9-4.2 Organic acids 1.6-1.8 Amino acids Example 2. K 94,8 weight.h. wood hydrolyzate, which contains 1% of reducing substances, is added 5.0 hours of acid-base extract and mineral salts containing 0.22 hours of nitrogen and up to 0.02 hours of phosphorus. The strain Candida sp. CR-9. Yeast is grown in aerator with stirring for 48 hours at 28-30 ° C. After completing the test, the yeast is filtered and dried to constant weight. The action of the biostimulant is judged; by the biomass yield of the yeast compared with the control (wood hydrolyzate), and the quality of the yeast by the protein content. The table shows the formulation of nutrient media and the results obtained when growing on them protein feed yeast. As can be seen from the table, the product obtained by the method proposed with the introduction into the nutrient medium (the tree of hydrolyzate and nutrient salts) contributes to a 70-80% increase in the yeast biomass yield compared with the prototype by 50%

The use of the proposed method of obtaining a biostimulating additive and peat in comparison with existing methods allows without the use of additional technological equipment and expensive 6997964

operations to obtain a product with high biological activity.

To teach a huge reserves of peat, based on it, you can create the production of the indicated bio-stimulator, which will ensure the need of the microbiological industry for this product.

Claims (1)

METHOD FOR PRODUCING FODGER YEAST GROWTH BIOSTIMULATOR, which involves hydrolysis of peat with mineral acid, neutralization of hydrolysis with subsequent separation of the solid residue, which involves treating the hydrolyzate with sodium hydroxide to separate the biological residue to separate the solid residue. to a medium pH of 12-13 and maintained at 38-40 ° C for 50-60 minutes, and the solution obtained after separation of the solid residue is neutralized and evaporated to a solids content of 45-50%. 05 СО СО m СО СО 05 microbi frequent