SU697925A1 - Method of calibrating polarographic oxygen cell - Google Patents
Method of calibrating polarographic oxygen cellInfo
- Publication number
- SU697925A1 SU697925A1 SU772491286A SU2491286A SU697925A1 SU 697925 A1 SU697925 A1 SU 697925A1 SU 772491286 A SU772491286 A SU 772491286A SU 2491286 A SU2491286 A SU 2491286A SU 697925 A1 SU697925 A1 SU 697925A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- calibrating
- oxygen
- oxygen cell
- polarographic oxygen
- polarographic
- Prior art date
Links
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
Изобретение относитс к области аналитической химии, а именно к пол рографическому определению кислорода , и касаетс ;калибровки, то есть определени чувствительности пол рографической чейки по кислороСпособ позвол ет по измеренным ве личинам токов чейки рассчитывать аб солютное значение содержани кислорода в водном растворе и может быть использован при исследовании процессов фотосинтеза и дыхани в суспензи х микроорганизмов и клеточных органелл ,а также во всех тех случа х,где необходимо непрерывное измерение концентрации кислорода в водных растворах . Известеи способ калибровки пол ро графических кислородных чеек с использованием в качестве титранта вос становленного никотинамид-аденин-динуклеотида (NADH). Взаимодействие добавл емого восстановител с растворенным кислородом проводитс в при сутствии медиатора - феназинметосуль фата (PMS) и фермента-- каталазы 1 Недостатком известного способа вл етс высока стоимость реагентов и сложность процесса, обусловленна как многокомпонентностью системы, так и тем, что титрование растворенного кислорода восстановленным NADH про .водитс в присутствии каталазы, активность которой мен етс при хранении. Изменение активности фермента может оказывать вли ние на результаты титровани . С целью упрощени процесса и повышени надежности калибровки предлагаетс в качестве титранта использовать раствор аскорбиновой кислоты, а титрование проводить при рН реакционной среды равном 10-12. Способ осуществл етс следуюсцим образом. В герметизированной реакционной камере, где концентраци кислорода, растворенного в среде, непрерывно измер етс с помощью пол рографической чейки, производитс титрование кислорода точным раствором аскорбиновой кислоты. Среду дл калибровки предварительно подвод т к рН 10-12 добавлением нескольких капель 2 н. КОН и насыцают кислородом при температуре опыта. Операцию насыщени провод т в отдельном сосуде путем пропускани потокаThe invention relates to the field of analytical chemistry, in particular to the polarographic determination of oxygen, and concerns calibration, i.e., determining the sensitivity of a polarographic cell using an oxygen method, allows the absolute value of the oxygen content in an aqueous solution to be calculated using measured values of cell currents and can be used in the study of photosynthesis and respiration in suspensions of microorganisms and cell organelles, as well as in all those cases where continuous measurement of concentration is necessary oxygen in aqueous solutions. Izvesti is a method for calibrating polar graphical oxygen cells using reduced nicotinamide adenine dinucleotide (NADH) as the titrant. The interaction of the added reducing agent with dissolved oxygen is carried out in the presence of a mediator - phenazine methosulphate (PMS) and the enzyme - catalase. 1 NADH is produced in the presence of catalase, the activity of which varies during storage. Changes in enzyme activity can affect the titration results. In order to simplify the process and increase the reliability of calibration, it is proposed to use an ascorbic acid solution as a titrant, and titration is carried out at a pH of the reaction medium equal to 10-12. The method is carried out in the following manner. In a sealed reaction chamber, where the concentration of oxygen dissolved in the medium is continuously measured using a polarographic cell, oxygen is titrated with an exact solution of ascorbic acid. The calibration medium is pre-adjusted to pH 10-12 by adding a few drops of 2N. KOH and saturated with oxygen at the temperature of the experiment. The saturation operation is carried out in a separate vessel by passing a stream
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU772491286A SU697925A1 (en) | 1977-06-01 | 1977-06-01 | Method of calibrating polarographic oxygen cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU772491286A SU697925A1 (en) | 1977-06-01 | 1977-06-01 | Method of calibrating polarographic oxygen cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU697925A1 true SU697925A1 (en) | 1979-11-15 |
Family
ID=20711211
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU772491286A SU697925A1 (en) | 1977-06-01 | 1977-06-01 | Method of calibrating polarographic oxygen cell |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU697925A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0214846A2 (en) * | 1985-09-06 | 1987-03-18 | Tytronics Incorporated | Sample monitoring instrument for on-line application |
-
1977
- 1977-06-01 SU SU772491286A patent/SU697925A1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0214846A2 (en) * | 1985-09-06 | 1987-03-18 | Tytronics Incorporated | Sample monitoring instrument for on-line application |
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