SU686738A1 - Mycobacterium tuberculosis bk-harkov strain - Google Patents

Description

The strain of Mycobacterium tuberculosis (BC-Hakow No. 841, a variant of the Bovis type Vallee strain, intended for the manufacture of a tuberculosis vaccine). The invention relates to veterinary medicine and is a new strain of Mycobacterium tuberculosis that can be used to prepare anti-tuberculosis vaccine preparations, other anti-genes, as well as serum preparations. . For the prevention of tuberculosis in people all over the world, a live vaccine of Mycobacterium tuberculosis is used. BCG / BCG strain (H) In veterinary medicine, vaccine prophylaxis in VTsZh in the Soviet Union and in most countries of the world is not currently implemented. One of the main reasons for this, in addition to the lack of immunogenicity of BCG, is the occurrence of prolonged allergies in BCG vaccinated animals that persist for a year or more, and the lack of practical methods for differentiating postvaccinal reactions to tuberculin. One of the means to improve vaccine prophylaxis of tuberculosis in humans and to create a drug for such individuals is the use of new, more immunogenic, vaccine strains of mycobacterial tuberculosis. These strains must be harmless and have sufficient immunization properties to protect those vaccinated from developing the tuberculosis process, and also not cause long-lasting allergies. The aim of the invention is to obtain a Mycobacterium tuberculosis strain that meets the main requirements for vaccine vaccines: harmless, sufficiently immunogenic to protect those vaccinated from developing the tuberculosis process. The resulting strain of Mycobacterium tuberculosis BC-Kharkiv has the following morphological, cultural and other biological characteristics. Morphological features: slightly curved, sometimes with thickening at the ends, polymorphic, gram-positive rods, 2-8 µm long and 0.2-0.5 µm thick, are typically colored with the Tsil-Nielsen method, stably retaining this color when exposed acids, alcohol and alcohol, not forming a spore and not having a capsule. The cytoplasm of bacteria has a homogeneous appearance and contains granules, microgranules and vacuum culture properties: on the surface of liquid nutrient media a friable, wrinkled film of light yellow color is formed. On dense nutrient media (Petrova, Vinogradov) it grows in the form of isolated R-type colonies with the formation of a lemon-yellow pigment. Grows under aerobic conditions. Anaerobiotic properties are not noted. The optimal temperature parameters are 37.8-38.0 С pH 4.58, 0. Biochemical properties: reduced activity of catalase, urease and dehydrase. Immunogenic properties: the protection index of BC-Kharkiv in an experiment on marine Evinki after a single subcutaneous injection of 5 mg BC-Kharkov and the subsequent infection of 0.01 mg of a virulent culture of Mycobacterium tuberculosis and strain of the body on the bodies after 2 times intracutaneous vaccination of 2 mg / kg or triple subcutaneous vaccination in doses of 0.4-0.6-0.8 mg / kg calf weight with an interval of 5 days and experimental infection with 15 mg of subcutaneously virulent culture of strain No. 8, or natural infection after prolonged contact with patients Tammy TB epidemics bodies, reaches 100%. Residual virulence: insignificant, primary tuberculosis complexes are not formed. Allergic properties: allergies are mild and relatively short-lived (up to two in guinea pigs, and up to six months in t). The virulent properties acquired by the Mycobacterium tuberculosis strain BK-Kharkiv turned out to be persistent, hereditarily fixed. Reverse passaging for 10 years did not reveal its original virulent characterization. Strain BKHarkov was obtained from the virulent Mycobacterium tuberculosis strain Vallee, of bovine type, which was passaged for 1-1.5 years on myc-peptone-glycerol broth containing 3-15% of the drug Ekteritsid. (vr Dissoluble oxidation products of fish oil in physiological saline sodium chloride), and the concentration of the extercid in the growing medium of mycobacterium tuberculosis was increased from 3 to 15% as the number of passages increased. Comparative characteristics of the strains of Mycobacterium tuberculosis BC-Kharkiv and BCG are given in the table.

In guinea pigs and tel t 100% to 2, and in tel t to 6

In humans, 73.2-83.7%

Life Cycle Vaccinated bodies only survive control animals, and 3–9 months after infection, they develop encapsulated tuberculosis.

As can be seen from the table, BC-Kharkiv is different from the BCG production strain by high immunogenic activity (protection index 100%), almost complete apathogenicity (primary tuberculosis complex is not formed), areactogenicity, and also insignificant guinea pigs

Primary tuberculosis complexes do not form months.

Primary tuberculosis complexes are formed. They are encapsulated and then resolved (disappearance time from 100 days to 4 years)

Claims (1)

telny allergenicity is a relatively short-term and mild allergic reaction to tuberculin, which makes it possible in practice to differentiate between animals vaccinated and infected with tuberculosis. The marked advantages of strain / BC-Khar compared with BCG were established on laboratory animals and on 300 bodies. Strains BC-Kharkiv is harmless to the calves and possesses immunogenicity. In laboratory experiments on the determination of immunity in 60 bodies vaccinated with various methods - intracutaneously, subcutaneously and orally - immunity was established, according to the vaccination method, in 100-40% of animals with artificial infection (lack of immunity was observed in bodies that la applied orally). In the production conditions of 489 chickens vaccinated intradermally and studied by an allergic method after vaccination, 20 to 70% of the animals did not react to tuberculin (in groups), and in three bodies, five out of five who reacted positively to tuberculization and killed for research after 6-9 months. after vaccination, pathological changes in the internal organs and lymph nodes typical of tuberculosis were found. The economic effect of using the vaccine BK-Kharkiv for the prevention of a tubule of cattle only in cattle across the country can reach hundreds of millions of rubles a year, since the death of one calf under the age of two years costs 350-500 rubles, depending on its breed, weight, cost and other indicators in each host. Claims of the invention The Mycobacterium tuberculosis BK-Kharokov strain, a variant of the Bovis type Vallee strain, intended for the preparation of a tuberculosis vaccine, is stored in the collection of the Kharkiv Scientific Research Institute of Microbiology, Vaccines and Serums. Mechnikov. Morphological features: slightly curved, sometimes with thickening at the ends, polymorphic, gram-positive rods 2–8 µm long and 0–0.5 µm thick Osh, 2–0,5 µm, typically stained by the Tsil-Nielsen method, stably retaining this color when exposed to acids, alkali and alcohol, not forming a spore and not having a capsule. The cytoplasm of bacteria has a homogeneous appearance and contains granules, microgranules and vacuoles. Cultural properties: a loose, wrinkled film of a light yellow color is formed on the surface of liquid nutrient media. On dense nutrient media (Petrova, Vinogradov) it grows in the form of isolated co. 10NII type R with the formation of a pigment of lemon-yellow color. Grows under aerobic conditions, anaerobiotic properties are not observed. The optimal temperature parameters are 37.8-38.0 ° С, pH - 4.5-8.0. Biochemical properties: the activity of catalase, urease and dehydrase is reduced. Immunogenic properties: guinea pig and tel reaches protection index. 100%. Residual virulence: negligible. Primary tuberculosis complexes do not form. Allergic properties: allergies are mild and relatively short-lived (up to two in guinea pigs and up to six months in tel). Sources of Information Accepted to the Attestation of Expert Examination 1. Yu. K. Weisfeiler, Guide to whey and vaccine matters. - M., 1943, p. 241253.