SU622468A1 - Method of separating biologically active fraction of liquid animal sperm - Google Patents
Method of separating biologically active fraction of liquid animal spermInfo
- Publication number
- SU622468A1 SU622468A1 SU762389766A SU2389766A SU622468A1 SU 622468 A1 SU622468 A1 SU 622468A1 SU 762389766 A SU762389766 A SU 762389766A SU 2389766 A SU2389766 A SU 2389766A SU 622468 A1 SU622468 A1 SU 622468A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- biologically active
- active fraction
- liquid animal
- animal sperm
- separating biologically
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
(54) СПОСОБ ВВДЕЛЕНИЯ БИОЛОГИЧЕСКИ АКТИВНОЙ ФРАКЦИИ ИЗ СЕМЕННОЙ ЖИДКОСТИ ЖИВОТНЫХ(54) METHOD FOR THE INTRODUCTION OF BIOLOGICALLY ACTIVE FRACTION FROM SEED SEED ANIMAL FLUID
Изо6| етение относитс к способам вьоделенй .биологически активных биогенных веществ, в частности к извлечению биологически активной фракции семенной жидкости животных.УказаннаЗ фракци может найти применение в животноводстве , например, дл синхронизации половой охоты домашних животныхIso6 | The study of genes refers to methods for the modeling of biologically active nutrients, in particular, the recovery of the biologically active fraction of seminal fluids of animals.
Известен способ выделени фракции простогландинов (ПГ) из предстатеоаьной железы баранов ij There is a method for isolating the prostaglandin fraction (PG) from the prostate gland of rams ij
ПО; этому способу ПГ ВЫДеЛЯЮТ ИЗ BY; this method PG excrete
замороженных предстательных желез баранов путем многократной экстракции эфиром, ;многоступенчатого противообмена между эфирной и буферной фазами и хроматографического разделени с промежуточным вакуумным упариванием экстрактов.frozen prostate glands of rams by multiple extraction with ether; multi-stage anti-exchange between the ether and buffer phases and chromatographic separation with intermediate vacuum evaporation of the extracts.
Недостатком.Х:казанрого способа извлечени биологически активных фракций вл етс его сложность и наличие большого числа технологичас .ких операций, что не позвол ет использовать его в крупном,масштабе.A disadvantage. X: The Kazan method of extracting biologically active fractions is its complexity and the presence of a large number of technological operations, which prevents its use on a large scale.
Цель изобретени - упрощение choсо .ба без снижени биологической активности получаемой фракции.The purpose of the invention is to simplify chosob. Without reducing the biological activity of the resulting fraction.
Это достигаетс тем, что перед экстракцией к семенной шдкЪстиThis is achieved by the fact that before extraction to the seed
добавл ютэтанол, декантируют водноспиртовую смесь и подвергают ее обработке гидроокисью кальци , полученный осадок регенерируют кислотой при одновременном добавлении к нему эфира дл проведени активной фракции.ethanol is added, the alcohol-alcohol mixture is decanted and treated with calcium hydroxide, the resulting precipitate is regenerated with acid while ether is added to it to conduct an active fraction.
Предлагаемый способ осуществл нп: следующим образом.The proposed method is implemented as follows.
Семенную жидкость заливают этанолом , перемешивают и отстаивают. Водно-спиртовую смесь после декантации и промывки концентрируют вакуумнЕЛм упариванием при.комнатной температуре . Упаренный раствор обрабатывают гидроокисью кальци и отдел ют осадок от раствора. Сухой осадок регенерируют сол ной кислотой с одновременной экстракцией эфиром. Эфирный раствор жирных кислот после отделени и высушивани упаривают в вакууме и последовательно подвергают фракционному разделению в хроматографической колонке,. Отбор фракций на колонки ведут элюэнтом,.,. содержащим бензол: тИлацетат-метанол в разных соотношени х .Seminal fluid is poured with ethanol, mixed and defend. After decanting and washing, the aqueous-alcoholic mixture is concentrated by vacuum evaporation at room temperature. One stripped off solution is treated with calcium hydroxide and the precipitate is separated from the solution. The dry precipitate is regenerated with hydrochloric acid and extracted simultaneously with ether. The ether solution of the fatty acids, after separation and drying, is evaporated in vacuo and subsequently subjected to fractional separation in a chromatographic column. The selection of fractions on the columns are eluent,.,. containing benzene: tI-acetate-methanol in different ratios.
Известно, что сами ПГ .групп- Е и Г обладают слабым поглощением в УФспектре . Однако при длительном нагревании в .присутствии щелочи они изоIt is known that the PGs .group-E and G themselves have a weak absorption in the UV spectrum. However, with prolonged heating in the presence of alkali they are
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762389766A SU622468A1 (en) | 1976-07-21 | 1976-07-21 | Method of separating biologically active fraction of liquid animal sperm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762389766A SU622468A1 (en) | 1976-07-21 | 1976-07-21 | Method of separating biologically active fraction of liquid animal sperm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU622468A1 true SU622468A1 (en) | 1978-09-05 |
Family
ID=20671776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU762389766A SU622468A1 (en) | 1976-07-21 | 1976-07-21 | Method of separating biologically active fraction of liquid animal sperm |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU622468A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2669693C1 (en) * | 2017-08-14 | 2018-10-15 | Павел Андреевич Канаев | Method for obtaining a complex of biologically active peptides that restore the prostate gland function |
-
1976
- 1976-07-21 SU SU762389766A patent/SU622468A1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2669693C1 (en) * | 2017-08-14 | 2018-10-15 | Павел Андреевич Канаев | Method for obtaining a complex of biologically active peptides that restore the prostate gland function |
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