SU596615A1 - Apparatus for growing microorganisms - Google Patents

Description

one

The invention relates to devices for carrying out the processes of growing microorganisms and may find application in the case of growing them on the basis of hydrocarbons.

Known devices of this type are known, containing a tank inside which an agitator is installed, made in the form of a shaft with river disks fixed on it, pipes for feeding nutrients, aeration gas for exhaust gas removal, the finished product, as well as separation elements 1 j.

In these devices, the process of growing microorganisms and their ion concentration (enrichment) takes place simultaneously, and the microorganisms are removed from the device along with the nutrient solution. The separating elements, installed in the apparatus and made in the form of perforated inserts with valves and gas pockets, serve to ensure the distribution of gas in the overlying volume and prevent re-mixing of the grown microorganisms.

In the prior art apparatus, the prepared product is not concentrated before sampling and is a mixture of microorganisms, water, nutrient solution and gas. Such devices are sometimes provided with a special tank for concentrating microorganisms.

The aim of the invention is the removal from the apparatus of the finished product in the form of a concentrate of microorganisms.

For this purpose, there is at least one zone of concentration of microorganisms and removal of the finished concentrate in the vessel. This zone is separated from the remaining volume of the tank by a perforated partition and is located above or below the stirrer discs in the upper or lower part of the tank.

FIG. 1 shows schematically the proposed apparatus with a microorganism concentration zone placed above the stirrer discs; in fig. 2 — apparatus with a microorganism concentration zone placed under the stirrer discs; in fig. 3 - apparatus with microorganism concentration zones, placed vs above and below the stirrer discs.

The aprat is made in the form of a vertical tank 1, inside which an agitator is installed, which is a rotary shaft 2 with a set of disks 3 installed on it 3. The tank 1 is equipped with nozzles 4, 5, 6 and 7, respectively, for supplying nutrients, aerating gas, exhaust gas and the finished product. On the inner surface of the vessel are fixed rings E. Each of the latter is installed under the corresponding drive 3.

A manifold 9 is connected to nozzle 5 for supplying gas, for example oxygen, to the tank. In the tank there is a zone 10 for the concentration of microorganisms and removal of concentrate, which is separated from the rest of the tank by a perforated partition 11.

This zone can be located above or below the disk 3 mixers. In the case of placing it above the disks 3 above the partition 11, drain channels 12 are installed for the concentrate of microorganisms to which the pipes 7 are connected for removal of the finished product.

The partition 11 may be made of tulle or mesh. Its purpose is to cause a decrease in kurbulence and to ensure the concentration of the finished product.

During the operation of the apparatus, a pipe 4 at the inside of the tank 1 is fed with a mixture containing a nutrient solution, water and a source of carbon-diesel fuel. . . . L

The pipe 5 and the manifold 9. in the tank -1 enters the gas. The initial product mixes well with microorganisms using discs 3 and rings 8. At the same time, air is distributed.

The aqueous phase in the zone under distilling 11 contains 28-30% of microorganisms.

A portion of the aqueous phase with air passes through the partition 11 and is fed to the ZONE 10 of concentration. At the same time, exhaust air is removed from the tank 1 through the pipe 6. In this zone, the concentration of the aqueous phase is increased to 38–40% of microorganisms in it as a result of the separation of a part of the nutrient solution. The concentrate is discharged from the tank through the drain channels 12 and the nozzles 7.

The liquid phase (nutrient medium and water), released mainly from microorganisms, enters the part of the capacitor located under the partition. 11. The concentration zone 10 may also be located under the stirrer discs 3 (FIG. 2), and there may be two of them. In the latter case, one concentration zone 10 is located above the disks 3 of the agitator, and the other - under them (FIG. 3).

Thus, in the proposed apparatus is not only the cultivation of microorganisms, 1 but their concentration in the aqueous phase.

For example, if before entering zone 10, the solids content in the aqueous phase was 9-12 g per 1 kg, then after concentration in zone 10 it increased and the concentrate removed from the apparatus was 1622 g dry matter per 1 kg of medium.

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Claims (1)

1. Patent of Germany 9 1642594, cl. Ba 15/02, 1976. .one sz - & -r h-f -Yes sz Zy L -Yes