SU591119A3 - Virus-insecticide composition and method of preparing same - Google Patents

Description

(54) VIRUS-INSECTICIDAL COMPOSITION AND METHOD FOR ITS OBTAINING

Next virosis: ppiaaroses, ntrroplasmvt (Chesqua polyaeroses, gravulosis and insect pox viruses.

Preferably, insecticides containing polyhedra viruses of the CPR (nucleic polyhedrosis virus-NIP) are used for Hettoth. Cs eeoL / HitCotKCs vtiescens, Trtditoptuuci nt. Uaplxye-ma f rug-lperda and LapKyiy Yna entdna.

Also of interest is the granulosa virus (HB) with Lc & peyt esici pOTno-neltoi, A chicep o-rQ-yrofipttui and Afg yrdtatntq

By virus-insecticide is meant only the self-contained body itself, but also its other destroyed organic materials; They can be separated from the closed pte a, but also not turn out to be negative. As a result, a separate particle and composition as a whole can have a relatively low content of pathogenic virus.

The lower limit of the activity equivalence of the composition corresponds to at least LB 50 - f / ml The activity can vary in the range of O, OO1 - 1.0 V / ml, preferably, OOOZ - O, 4 G / ml.

The proposed virus-insecticidal composition is prepared by spray drying VOSHYO mixture containing 0.1 - 35 weight. % virus insecticide and 6599, 5 weight. % matrix material consisting of an inert protein material and / or clay. The temperature of the aqueous mixture, which is introduced into the spray dryer, can range from 12 to 38 ° C at a temperature of 49 to 138 ° C. The mixture can be fed to a spray dryer under a pressure of 0.34 -. 3.0 atm, preferably 0.64 - 2.5 atm.

The spray drying process is faster at low temperatures. Temperature in the spray; the zone must not exceed. The temperature difference between j $ iBp BO; yi is relative. HipehibBonepeiM "w p may be lOSoC;

Heat for: the p; 8 spray drying process is usually provided by supplying heated inert gas that does not adversely affect the constituent parts of the insecticide virus; you can use the air.

The duration of the drying process at 49 ° C and above should not exceed 15 seconds, preferably 10 seconds.

The aqueous mixture fed to the spray dryer is an aqueous suspension of solid constituents (rus-insecticide and matrix material), the content of which should not exceed 3 - 38% of the total weight of the aqueous mixture. All the solid components in the mixture are in a finely ground state, and the matrix material in possible additives in the dust-like state is able to pass through a 2OO mesh sieve and have a particle size of O, 1-5 O m, preferably O, 2 20 O m.

Preparations obtained from acetone are very fine-grained powders with a particle size of 0.5 - 18O µm. Insecticide viruses obtained by a non-acetone method are crushed in the presence of a certain amount of water in order to obtain a preparation that passes through a 50-15 mesh screen, preferably too much, And y. koTOJspr o a closed body is released from the carrier material or the carrier material is very finely crushed (0.5-1OO μm, preferably O, 515 μm).

Insect larvae containing the virus insecticide are converted to a water-dispersed state by conventional methods. The pH of the water dispersion of the virus-insecticide is 2.0-5.0, the optimum is 5.5-8.0.

The pH of the aqueous mixture is 5.5 - S, 0 supported by the addition of 1-12 wt.% (counting the weight of the virus-insecticide) of any known water-soluble inorganic ({usphate, for example, mono- and di-phosphate sodium or ml and.

The addition of inorganic phosphate to the viRa — the insecticide composition makes it more resistant to tiuyu and {fugimi influence of the environment. .

As matrices, it is more expedient to use a defatted 6–11 shaker mater, such as Myka soybeans (K -CO), defatted, flour, flour. From. cotton seed (proflo); About Ajn: Nu1b Flour from Seeds after Sunflower - Extracts of Differences: Nro scoLivev, | fvdaocht to a very deo-soy. b.eok. From animal proteins; you can use skim milk, casein, and egg albumin (drink bovine material in liquids. A substance containing not more than 25 wt.% Protein) .: ..:

The clay used in the composition may be finely divided or powdered. For example, kaolin clays (kaolin jeong-a), building clays, attapulgites and bentonites are suitable.

The weight ratio of protein to clay is from O, 1 to 10: 1, preferably from 0.5 to 3: 1,

Experiments have shown that the proposed composition is characterized by increased stability, wettability, hardness and / or adhesiveness. Test 1: UV stability test A sample of the composition was suspended in a multiple of water, placed in a Petri dish, incubated for 5C under UV light, and then insecticidal activity was measured. A comparison of the resultant activity with the initial one shows the measure of the UV content of the composition. OnbJT 2. Wettability Test. A pyrex glass beaker (10.5x8.5 SKU No. is placed near the cola of the level gauge, a POO of ml heated at 23 ° C is poured into it), a plastic funnel is prevented above the glass (outer diameter B cm, internal diameter of 1.6 cm ) - in such a way that the end of the funnel tube is 1O cm above the surface of the wall. O, 5g of the proposed composition is interfered with in the funnel and the time until the full moisture of the floating material is measured.The time of 6O s characterizes the good wettability of the composition. adhesive ability: Two drops 0.5 % of suspension: virus-insecticide composition is applied to the object carrier and ocrasmocrT for 2. h at room temperature ao about {M1zavayn dry coating. A thin layer visually heals well. The object carrier is then vyderzhayut under the top 45 at a depth It is used in water flowing at a rate of 10 ml of aa 15 s. The depth to which the layer is called flowing by water can be easily heated .. The relative amounts of visible washed flowing water and also glued at: the surface of the material is measured by measure. augge zyoTsvry-abilities.

Tb

a 1 Experience 4. Test the hardness of the particles. The diameter of the particles in the aqueous suspension was determined using a Petrov-Nansen-measuring instrument for bacteria under a 11-phase microscope. The hardness of the particles in one suspension is measured in the following way: O, 5% suspension | This is injected into a cylinder with a capacity of 100 ml and turned 30 times over 6O with the inverter up and down. The integrity of the particles is observed by the JcTepeoMnKpocKonoM .. soft particles disintegrating to form fine quasi-amorphous particles, while the solid particles retain a spherical shape. Part 1: Twice-split pores days yo 8O g of the hemp-like technical concentrate HeCLOtKCs exi, obtained by the acetone method, with biological activity calculated as L05o, 0, OO30, O6. G / ml of HetCotKts gvl, the first stage of the larvae was transferred with a wartnor watch glass in portions of BOO ml of water for 1 min. Various chemical compounds (see Table 1) are suspended in a II liter of air and mixed with one of the virus suspensions. The volume of each individual mixture is watered with water up to 15 liters and stirred until a homogeneous suspension is obtained. Virususpensions are spray dried with an inlet temperature of 160 ° C and an outlet temperature of 71 ° C, maintaining a pressure of 1.5-2.5 atm. The exit hole size of the nozzle (affects particle size) is N SA 0.044. Each of the resulting virus-insecticidal compounds j is tested for activity, wettability, adhesiveness, and particle strength. The results are shown in table 1.

Building

clay

Kaolin

K-so

Clear milk

Building

1500 + 8200.15 clay + kaolin

Solid

Good

Good

Soft

Solid

Uniform

Amorphous

Uniform

Solid

Good

Clay + K-Co

150O + 820

K-yoo builder “Ypus” means that the composition is glued “: 5, Example 2. A mixture consisting of 80 g of powdered technical concentrate HettOth.ts not produced by the acetone method, 50 g of dihydrophosphate / potassium and 2.0 g of magnesium sulfate heptagidrite, mixed with the components indicated in Table 2, add 1 l of water, mix until a homogeneous suspension is formed, then each of the three suspensions is 7100 + 4100 O, 12 clay + K-chzo C2OO0,11 Proflo, Building 71OO + 41OO O, 12 clay + profile Voron / N + technical concentrate of lactose powder (TCPL). 6 (%) + 94 (%)

8 Continuation of table 1

0.12

Good

Solid minus washes out. Add water to 5.28 L and adjust the pH to 6.5 by adding potassium hydroxide. Next, the suspensions are spray dried as described in Example 1, and the activity and physical characteristics of the resulting virus-insecticidal compositions are determined. The control is a virus-insecticide riron / N with the addition of powdered lactose. The results are presented in table 2. Table2 45Good Solid5-25 50Good Solid5-25 35Good Solid5-25 90Aniform Amorphous

h p and ker 3, Two mixtures containing 5 rbb g each of Hetioth-Cs frozen larvae infected by the virus, gya, are suspended in 1.58 liters of water, mixed for 2 minutes. Homogenous mixtures of fil1 / grupa through sno (OOmesh) OLYA removal of insect cell debris, 6O g of potassium digiprophosphate, 2.0 g of magnesium sulphate, 7.0 g of hot water components are added to the crude virus preparation.

Build to

6OOO + 525O Solid 5-25 35 clay + K-s Kaolin + proflo 62OO-50OO vryvye PRIMER 4. Spray-dried compositions described in examples 2 and 3, pend in a multiple of water, in-30 interfere in petri dishes and kept under

Frozen larvae +

Oh, 11 + clay + K-co

Frozen larvae +

 0.13 + kaolin + proflo

those indicated in Table 3 are then diluted in water to 5.284 l. The pH is adjusted to 6.5 by the addition of potassium hydroxide. Spray drying is carried out at the input temperature of 93.3 ° C, output 6 ° C and pressure of 10-1.3 atm (nozzle A 027),

The test results obtained virus insecticide compositions are given in table.3.

Table3

0.103

Good

Oh 109

74

Oh 15

87

0.15 5-25 45 Good by UV light. Then the UV stability of the virus-insecticidal compositions is determined. For comparison, test the known composition Viron / H, R. desugates are given in table 4, T a b l and c a 4

PRI me R 5. BbicjrmeBHbie by spraying the preparations of Example 2 and 3 are stored at 5 ° C and at 38 ° C. then after some time they are extracted and their thermal stability is determined. Residual

Frozen larvae + clay + K "h; o

Frozen snacks "Kaol n + profile" The nutrient medium in which the tests were carried out in the above experiments, has the following composition of ml: distilled during the HHHE; methylparas cs-benzoate (15% w / v 9% alkyl alcohol) 36; cholium chloride by contontracion (0.1 g / ml water) 36; potassium hydroxide (4M) 18f formalin (O, 1 g / m 25; vitamin concentrate, consisting of 6OO mg of niain, 6OO mg of calcium pantothenate, ZOO mg of riboflavin, 15O mg each of 1Tiamine, pyroaoxin and folic KEYULOTA, 12 mg of biotin, 1.2 mg of vitamin B 1OO ml of water and water extract, 6. I. / .HqjManbuae contains, rr kaeek willows 126 suprrza 3.26 rtkv BiiieHHm i 1О8, ager Wessow 36, aptFvtsel 18, ascorbic kvpot also avgvbiotiki (chlorotetracnkln kavBMNpnv) | Similarly to example 3, an aqueous mixture of 8% virus drug (N. t), which contains a certain number of destructive cells (passed through a sieve of 1OO mesh), 56%, 30% ATTaclb (attapuliit) and 60% potassium dihydrophosphate. Total solid content substances in an aqueous mixture of 20 wt.%. The particles are solid, about

authentic drugs; lasts longer after storage at 3S C (half-life of decay is more durable). The results are presented in table. five.

Tablvpa 5

38.5

10 28, O

Claims (4)

9 are able to be wetted and, with the necessary adhesion properties) “have LD 5c X) E) 1-b, 1 G / ml. It should be noted that spray-drying of aqueous sms and virus-insecticidal compositions contain from 3 to 1 O weight. % moisture - usually.%. The formula of the invention and the 1.Virus-insecticidal composition with characteristic virus particles is about that it contains 0.1-35 wt.% Wc), which is a polyhedrony nuclear one. virus, preferably fie tOfchC &. ti, 6599, 9 wt. % of matrix composed of protein material and / or clay ... 2. The composition according to claim 1 is distinguished by the fact that the size of the virus particles is 3-150 µm, preferably 5-50 µm. 3. The composition according to P, 1, O tl and h and y and «with the fact that it contains 1-12 wt% of any known inorganic inorganic phosphate. 4. The method, which is applied to the insecticide compound, is also due to the fact that it is a different mixture with a content of 0, weight,% virus-insecticide, 65-99.9 weight. % of matrix material consisting of inert protein material (clay), the viral strain and matrix material, ct, being 3-35% by weight of the mixture, spray dried. 5. A method according to claim 4, characterized in that the water mixture to be injected is spray dried at a temperature of 12-38 C and at an output temperature of 49138 C. 6, The method according to claim 4, which distinguishes t; in that the retention time of the mixture under spray drying conditions is less than 15 s. 59 7, the method according to claim 4, that is, that the pH of the mixture is 5.5-8.0. Sources of information taken into account in the examination: 1.Orlovska E.V. Insect viruses and (their use in plant protection. A review of geography, M., 197O, 2.Orlovska E.V., and Sefikhanov Sh.S. Economic evaluation of the use of the drug rhyrin ench. Forestry, 1974, No. 6, Ju.91-93. 3.Dikasova E, T. and Talipov K.A. Raa | a) the method of making a viral preparation. - Uzbek Biological Journal, 1975, N 6, p. 60-61. . 4. JOurTiat of Anvertebrate athoWJy, 1965, N 2, p. 217-226