SU590665A1 - Method of investigating - Google Patents
Method of investigatingInfo
- Publication number
- SU590665A1 SU590665A1 SU762324231A SU2324231A SU590665A1 SU 590665 A1 SU590665 A1 SU 590665A1 SU 762324231 A SU762324231 A SU 762324231A SU 2324231 A SU2324231 A SU 2324231A SU 590665 A1 SU590665 A1 SU 590665A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- phase
- coagulation
- blood
- blood plasma
- investigating
- Prior art date
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- Investigating Or Analysing Biological Materials (AREA)
Description
Изобретение относитс к области медицины, а именно к исследованию биологических сред, например крови, и может быть использовано дл получени информации о физиологическом состо нии организма и патологических процессов, происход щих в нем.The invention relates to the field of medicine, namely to the study of biological media, such as blood, and can be used to obtain information about the physiological state of the organism and the pathological processes occurring in it.
Известен сиособ исследовани процесса свертывани крови, заключающийс в том, что в пробу сыворотки крови добавл ют хлористый кальций и регистрируют изменение в зкости иолучеиной сыворотки :.A known method for studying the process of blood coagulation is that calcium chloride is added to a blood serum sample and the change in viscosity of the i-ray serum is recorded:.
Однако известный способ не обеспечивает выеокой точиости исследовани .However, the known method does not provide accurate research.
Целью изобретени вл етс повышение точиости исследовани путем уменьшени искаже )ий ироцесса свертывани .The aim of the invention is to increase the accuracy of the study by reducing the distortion of the coagulation process.
Это достигаетс тем, что регистрацию изменений в зкости иронзвод т по измерению теплопроводности сыворотки.This is achieved by recording the changes in viscosity of the iron by measuring the thermal conductivity of the serum.
Пример. В кювету тромбоэластографа заливают плазму крови в разведении физиологическим раствором при соотношеиии 1 : 10 в объеме 0,15 мл. Кюветупомещают в вод ную баню. Микротерморезистор, нанример, типа МТ-64 погружают в кювету до соприкосновени с поверхностью разведени илазмы крови . Затем в кювету заливают хлористый кальций в концентрации 0,0277 в объеме 0,15 мл и производ т измерени с помощью мостовой схемы.Example. Blood plasma is diluted with a saline solution at a ratio of 1:10 in a volume of 0.15 ml into a cuvette of a thromboelastograph. Ditch in water bath. A microthermistor resistor, such as the MT-64 type, is immersed in a cuvette until it comes into contact with the surface of the dilution of blood plasma. Calcium chloride is then poured into the cuvette at a concentration of 0.0277 in a volume of 0.15 ml and measured using a bridge circuit.
В процессе свертываип системы сопротивление микротерморезистора измен етс , что сказываетс на величине тока разбаланса моста . В измерительной диагонали мостовой схемы возникают изменени иаир жени , которые после усилени дифференциальным усилителем регистрируют на самописце.In the process of coagulation of the system, the resistance of the microdistor resistor changes, which affects the magnitude of the current unbalance of the bridge. In the measuring diagonal of the bridge circuit, iaire changes occur, which, after amplification by a differential amplifier, are recorded on a recorder.
На чертеже изображена термограмма, отражающа процесс свертывани плазмы крови в разведеиии 1 : 10 с СаСЬ.The drawing shows a thermogram reflecting the process of coagulation of blood plasma in a dilution of 1: 10 with CAC.
Крива показывает характерные фазы реакции .The curve shows the characteristic reaction phases.
I фаза составл ет 30-40 сек, она характеризуетс резким падепием сопротивлепи терморезистора и соировождаетс экзотермическим эффектом.Phase I is 30-40 seconds, it is characterized by a sharp increase in the resistance of the thermistor and has an exothermic effect.
Продолжительность II фазы колеблетс в более широком временном интервале и дл плазм крови различных больных составл ет 120-420 сек. Эта фаза характеризуетс структурными изменени ми исследуемой системы , вызывающими нарушение тенлового баланса , но не оказывающими существенного вли ни на тепловые свойства системы в целом . Зубцы кривой демонстрируют изменени степени дисиерсионноети системы и нос т флуктуациониый характер. Эта фаза реакции отражает врем и особенности свертывани плазмы крови до момента образовани фибрина .Phase II duration varies over a wider time interval and for blood plasma of different patients is 120-420 sec. This phase is characterized by structural changes in the system under study, which cause a violation of the tenel balance, but do not have a significant effect on the thermal properties of the system as a whole. The teeth of the curve demonstrate changes in the degree of the system's dissierce system and are of fluctuation nature. This phase of the reaction reflects the time and features of blood plasma coagulation until the formation of fibrin.
III фаза - активное превращение фибриногена в фибрин. Длительность фазы 90- 120 сек. Эта фаза реакцнн сопровождаетс резким снижением конвективной составл ющей теплообмена в результате возрастани в зкости среды. В этой фазе наблюдаетс изменение коэффициента теплоотдачи на 15- 20%.Phase III - active conversion of fibrinogen to fibrin. The duration of the phase is 90-120 seconds. This phase of the reaction is accompanied by a sharp decrease in the convective component of heat exchange as a result of an increase in the viscosity of the medium. In this phase, a change in the heat transfer coefficient by 15-20% is observed.
Предлагаемый способ нсследовани свертывающей системы крови обесиечивает больщую точность измерений благодар исключению механического воздействи на процеес свертывани крови и повышает чувствительность в результате иомещени микротерморезистора с высоким температурным коэффициентом сопротивлени на границе раздела фаз свертывани и за счет учета измен ющейс конвективной составл ющей теплообмена.The proposed method of investigating the blood coagulation system provides greater measurement accuracy by eliminating the mechanical effect on the blood coagulation process and increases the sensitivity as a result of the placement of a microresistor with a high temperature coefficient of resistance at the coagulation phase interface and by taking into account the variable convective heat transfer component.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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SU762324231A SU590665A1 (en) | 1976-02-11 | 1976-02-11 | Method of investigating |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU762324231A SU590665A1 (en) | 1976-02-11 | 1976-02-11 | Method of investigating |
Publications (1)
Publication Number | Publication Date |
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SU590665A1 true SU590665A1 (en) | 1978-01-30 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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SU762324231A SU590665A1 (en) | 1976-02-11 | 1976-02-11 | Method of investigating |
Country Status (1)
Country | Link |
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SU (1) | SU590665A1 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0332110A2 (en) * | 1988-03-07 | 1989-09-13 | Snow Brand Milk Products Co., Ltd. | Method for measurement of viscosity change in blood or blood plasma and sensor therefor |
US6101449A (en) * | 1995-06-07 | 2000-08-08 | Akzo Nobel N.V. | Method for predicting the presence of congenital and therapeutic conditions from coagulation screening assays |
US6321164B1 (en) | 1995-06-07 | 2001-11-20 | Akzo Nobel N.V. | Method and apparatus for predicting the presence of an abnormal level of one or more proteins in the clotting cascade |
US6429017B1 (en) | 1999-02-04 | 2002-08-06 | Biomerieux | Method for predicting the presence of haemostatic dysfunction in a patient sample |
US6502040B2 (en) | 1997-12-31 | 2002-12-31 | Biomerieux, Inc. | Method for presenting thrombosis and hemostasis assay data |
US6898532B1 (en) | 1995-06-07 | 2005-05-24 | Biomerieux, Inc. | Method and apparatus for predicting the presence of haemostatic dysfunction in a patient sample |
US7179612B2 (en) | 2000-06-09 | 2007-02-20 | Biomerieux, Inc. | Method for detecting a lipoprotein-acute phase protein complex and predicting an increased risk of system failure or mortality |
US7211438B2 (en) | 1999-02-04 | 2007-05-01 | Biomerieux, Inc. | Method and apparatus for predicting the presence of haemostatic dysfunction in a patient sample |
-
1976
- 1976-02-11 SU SU762324231A patent/SU590665A1/en active
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0332110A2 (en) * | 1988-03-07 | 1989-09-13 | Snow Brand Milk Products Co., Ltd. | Method for measurement of viscosity change in blood or blood plasma and sensor therefor |
US6101449A (en) * | 1995-06-07 | 2000-08-08 | Akzo Nobel N.V. | Method for predicting the presence of congenital and therapeutic conditions from coagulation screening assays |
US6269313B1 (en) | 1995-06-07 | 2001-07-31 | Akzo Nobel N.V. | Method for predicting the presence of congenital and therapeutic conditions from coagulation screening assays |
US6321164B1 (en) | 1995-06-07 | 2001-11-20 | Akzo Nobel N.V. | Method and apparatus for predicting the presence of an abnormal level of one or more proteins in the clotting cascade |
US6564153B2 (en) | 1995-06-07 | 2003-05-13 | Biomerieux | Method and apparatus for predicting the presence of an abnormal level of one or more proteins in the clotting cascade |
US6898532B1 (en) | 1995-06-07 | 2005-05-24 | Biomerieux, Inc. | Method and apparatus for predicting the presence of haemostatic dysfunction in a patient sample |
US6502040B2 (en) | 1997-12-31 | 2002-12-31 | Biomerieux, Inc. | Method for presenting thrombosis and hemostasis assay data |
US6429017B1 (en) | 1999-02-04 | 2002-08-06 | Biomerieux | Method for predicting the presence of haemostatic dysfunction in a patient sample |
US7211438B2 (en) | 1999-02-04 | 2007-05-01 | Biomerieux, Inc. | Method and apparatus for predicting the presence of haemostatic dysfunction in a patient sample |
US7179612B2 (en) | 2000-06-09 | 2007-02-20 | Biomerieux, Inc. | Method for detecting a lipoprotein-acute phase protein complex and predicting an increased risk of system failure or mortality |
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