SU589293A1 - Method of preparing cocoons to unwinding - Google Patents
Method of preparing cocoons to unwindingInfo
- Publication number
- SU589293A1 SU589293A1 SU762360338A SU2360338A SU589293A1 SU 589293 A1 SU589293 A1 SU 589293A1 SU 762360338 A SU762360338 A SU 762360338A SU 2360338 A SU2360338 A SU 2360338A SU 589293 A1 SU589293 A1 SU 589293A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- cocoons
- wall
- unwinding
- preparing
- going
- Prior art date
Links
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- Peptides Or Proteins (AREA)
Description
етвами, процессы запаривани и размотки их проход т при одних и тех же режимах.These processes, the steaming and unwinding processes take place under the same modes.
Перед смешиванием от коконов каждой заготовительной партии отбираетса образец в 1ОО коконов, из которых путем вырезки и очистки приготавливаютс гробм коко ной оболочки. Дл электрофореза беретс навеска измельченной оболочки, котора неоднократно щ)омываетс дистиллиррванной водой за нваетс трисглициноиым буферным раствором и экстрагируетс в течение ЗО МИй в термостате при температуре 85 С, после чего экстрагнрова нный раствор вноситс в электрофорётическиё трубки с полиакриламйдным гелем, 6 которых проводитс электрофорез , Полиакриламидный гель, заполн ющий пространство между электродами, вл51етс токопровод щей средой , и во ррем электрофореза образуетс спектр компонентов испытуемого вещества.Before mixing, from the cocoons of each batch, a sample is taken into 1OO cocoons, from which the coffins of the cocoon are prepared by cutting and cleaning. For electrophoresis, a portion of the crushed shell is taken, which is repeatedly washed with distilled water, covered with trisglycine buffer solution and extracted into the thermostat at 85 ° C during 85 ° C, after which the extraalloyed solution is applied to the polyacrylamide gel, I am going to go to the wall and go to the wall, I’m going to use the 4-dimensional, 3-dimensional, green, gray, and three-dimensional diagrams that are imbedded in polyacrylamide gaiton, and I am going to go to the wall and go to the panel with the wall, I am going to the wall and go to the wall, I am going to the wall and go to the wall and go to the wall, I’m going to have the 4-diagonal goust, I am going to the wall and go to the wall and go to the wall, I’m going to use the 4-D, 3-g The polyacrylamide gel, which fills the space between the electrodes, acts as a conductive medium, and during the electrophoresis a spectrum of the components of the test substance is formed. properties.
По окончании фореза гели снимаютс и окрашиваютс красителем амид1ым черным , nooie чего избыток красител , не св занного с серицином, отмываетс 7%-ным раствором уксусной кислоты.At the end of the pore, the gels are removed and stained with amide black, the nooie of which excess dye not associated with sericin is washed with a 7% acetic acid solution.
После промывки уксуснойкислотой на участках гел , на которых концентрируетс серицин, в зависимости от зар да и мопекул рной массы, остаютс зоны белка в виде дисков, св занные с красителем и четко видимые на электрофореграммах.After washing with acetic acid on the areas of the gel on which sericin concentrates, depending on the charge and mycopulic mass, there remain areas of protein in the form of discs associated with the dye and clearly visible on electrophoregrams.
На основе полученных электрофореграмм объединению в группы подвергаютс коконы тех заготовительных партий, по которым получены сложные фореграммы, т.е. с одинаковыми электрофоретическими характеристками серицина.On the basis of the electrophoregrams obtained, the cocoons of those preparation lots for which complex forgrams were obtained, i.e. with the same electrophoretic characteristics of sericin.
При таком способе подготовки коконов к размотке достигаетс однородность коконов , объединенных по технологическим признакам. При этом выбранные режимы запаривани и размотки оказываютс едиными дл всех коконов, что приводит к снижению отходов (коконного сдира недомотанных коконов,), увеличению доматываемости оболочек и повышению выхода лелкаг-сырца.With this method of preparing cocoons for unwinding, uniformity of cocoons is achieved, united by technological features. In this case, the selected modes of steaming and unwinding are the same for all cocoons, which leads to a decrease in waste (cocoon stripping of non-hemmed cocoons), an increase in the habitat of the shells and an increase in the yield of raw silk.
и 3 о б р е т е Н-. и and 3 o b e e H-. and
Форм у лForm in l
Способ подготовки коконов к размотке путем из смешивани , очистка от сдира и пыли и рассортировки по технологическим признакам с последующим объединением в группы по однородности этих признаков, отличающийс тем, чjo, с целью упрощёнй процесса подготовки и повышени выхода шелка-сырца, перед смешиванием производ т электрофорез серицина оболочек в полиакриламидном геле, а объединение в группы осуществл ют с учетом электрофоретических характеристик.The method of preparing cocoons for unwinding by blending, stripping and dusting and sorting according to technological features, followed by grouping these features into groups for uniformity, characterized by that, in order to simplify the preparation process and increase the yield of raw silk, before mixing. electrophoresis of sericin shells in a polyacrylamide gel, and grouping is carried out with regard to electrophoretic characteristics.
Источники информации, прин тые во внимание при экспертизе:Sources of information taken into account in the examination:
1. Реф. научн , техн. сб. Шелк, 1974, № 1, стр. 23.1. Ref. scientific, tech. Sat Silk, 1974, No. 1, p. 23.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU762360338A SU589293A1 (en) | 1976-05-07 | 1976-05-07 | Method of preparing cocoons to unwinding |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU762360338A SU589293A1 (en) | 1976-05-07 | 1976-05-07 | Method of preparing cocoons to unwinding |
Publications (1)
Publication Number | Publication Date |
---|---|
SU589293A1 true SU589293A1 (en) | 1978-01-25 |
Family
ID=20661362
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU762360338A SU589293A1 (en) | 1976-05-07 | 1976-05-07 | Method of preparing cocoons to unwinding |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU589293A1 (en) |
-
1976
- 1976-05-07 SU SU762360338A patent/SU589293A1/en active
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