SU579307A1 - Method of cultivation of anaerobic microorganisms - Google Patents
Method of cultivation of anaerobic microorganismsInfo
- Publication number
- SU579307A1 SU579307A1 SU7402002816A SU2002816A SU579307A1 SU 579307 A1 SU579307 A1 SU 579307A1 SU 7402002816 A SU7402002816 A SU 7402002816A SU 2002816 A SU2002816 A SU 2002816A SU 579307 A1 SU579307 A1 SU 579307A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- cultivation
- anaerobic microorganisms
- labinska
- research methods
- microbiologists
- Prior art date
Links
Description
.U.U
MOB, так как химические компоненты питателглой среды, вводимые с целью обеспечени анаэробиоза, например резазурин, оказывает иигибирующее действие, а также отсутствием воаможности строго количест венного учата облигатных анаэробов, так как услови опыта не позвол ют обес11еч№гь полную изол цию облигатных анаэробов от в оздействи кислорода окружающей среды.MOB, since the chemical components of the nutrient medium introduced to ensure anaerobiosis, for example resazurin, have an inhibitory effect, as well as the lack of a strictly quantitative approach to obligate anaerobes, since the experimental conditions do not allow for the complete isolation of obligate anaerobes from the anaerobes The action of environmental oxygen.
Дл этого застывшую поверхность средр: привод т в контакт с предварительно ннф1шированной аэробами поверхностью твердого тела.For this purpose, the frozen surface of the medium is brought into contact with the surface of a solid body previously exposed to aerobes.
Осуществл етс это следующим образом.This is done as follows.
После подогрева кашшл ра с данной суспензией спор возбудител ботулизма содержимое высевают непосредственно в чашку Петри. Содержимое тщательно перемешивают с расплавленной питательной средоь (температура 45-50 ) и остбомх ют в горизонталь ном положении до .затвердевани среды. На; поверхность застывшей среды накладывают предметное Истекло, одну из г раней которого предварительно смачивают суспензией культуры аэробных микроорганизмов. Через 48 ч в центре под стеклом на рассто нии от кра стекла на 4-6 мм наблюдаетс и учитываетс рост колоний облигатных анаэробов.After preheating the cough with this suspension, the spores of the botulinum pathogen are sown directly into a Petri dish. The contents are thoroughly mixed with the molten nutrient medium (temperature 45-50) and left in a horizontal position until solidification of the medium. On; the surface of the frozen medium impose an objective Expired, one of which is pre-moistened with a suspension of a culture of aerobic microorganisms. After 48 hours in the center, under the glass, at a distance of 4-6 mm from the edge of the glass, the growth of obligate anaerobic colonies is observed and taken into account.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7402002816A SU579307A1 (en) | 1974-02-15 | 1974-02-15 | Method of cultivation of anaerobic microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7402002816A SU579307A1 (en) | 1974-02-15 | 1974-02-15 | Method of cultivation of anaerobic microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU579307A1 true SU579307A1 (en) | 1977-11-05 |
Family
ID=20577849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU7402002816A SU579307A1 (en) | 1974-02-15 | 1974-02-15 | Method of cultivation of anaerobic microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU579307A1 (en) |
-
1974
- 1974-02-15 SU SU7402002816A patent/SU579307A1/en active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR910012226A (en) | Methods of Biological Inactivation of Nucleic Acids | |
| SU579307A1 (en) | Method of cultivation of anaerobic microorganisms | |
| Ali et al. | Evaluation of siderophore produced by different clinical isolate Pseudomonas aeruginosa | |
| Boyd et al. | Presence of Azotobacter species in polar regions | |
| Spray | An improved anaerobic culture dish | |
| Smith et al. | Use of acetamide broth in the isolation of Pseudomonas aeruginosa from rectal swabs | |
| EP0104001A2 (en) | Triphasic mycoplasmatales culture device and method and competing microorganism inhibiting device for use therein | |
| Cowan | Micromethod for the methyl red test | |
| Glick | I. The Microbiology of Aging Clays | |
| GB1352545A (en) | Fermentation process | |
| Stuart et al. | Bacteriological studies on the “natural” fermentation process of preparing egg white for drying | |
| Ando et al. | STUDIES ON THE EFFECT OF TWEEN 80 ON THE GROWTH OF ERYSIPELOTHRIX LNCIDIOSA | |
| US3825474A (en) | Proteus resistant agar culture media | |
| ES488865A1 (en) | Preparation of 1-desoxynojirimycin. | |
| SU487111A1 (en) | The method of obtaining 6-methyl-2-naphthoic acid | |
| JPS5642587A (en) | Production of chlesterine esterase | |
| SU81072A1 (en) | Method for quantitative determination of vitamin BI | |
| SU1138409A1 (en) | Strain bacillus thuringiensis var. galleriae 114869/7 for use at test object for indication of bacteriophages | |
| CN103290096A (en) | Method for appraising starch decomposition capability of moulds | |
| Musílek | Adaptation of Escherichia coli to chlortetracycline during continuous cultivation | |
| ES2055728T3 (en) | A METHOD TO PRODUCE A NEW UREASA. | |
| Turner-Graff | An effective use of Petri dishes for microcultures | |
| SU960593A1 (en) | Photobacterium mandapamensis m-58,1651 strain used as test object for determination of amylosubtilin | |
| SU514891A1 (en) | The method of obtaining tartaric acid | |
| Groisman et al. | Coimmobilization of substrate and biocatalyst |