SU563432A1 - Apparatus for growing microorganisms - Google Patents

Description

(54) APPARATUS FOR CULTIVATION OF MICROORGANISMS

FIG. 1 shows the proposed apparatus for growing microorganisms, a vertical section; in fig. 2 is a section along A-A in FIG. one; in fig. 3 is a section along BB in FIG. one.

The device is a container 1 with expanded upper and lower parts and a central shell with a heat exchange jacket 2. Inside container 1, concentric with its vertical axis, the flow direction and circulation cups 3 and 4, made in the form of coils with coils wound without a gap, are installed and equipped with vertical baffle plates 5. In the upper part of the cups there are vanes b, and inside the circulation cuff 3 along its height there are perforated partitions 7 for dispersing the gas in a liquid.

In the upper part of the tank 1 there is a defoamer, made, for example, in the form of a collector 8, and a canle separator U. A device for desorbing carbon dioxide in the form of a 1U chamber with a perforated bottom 11 filled with ion-exchange resin is fixed above the antifoam agent. The ion exchange resin is fed through the nozzle 12, and is removed for regeneration through the nozzle 13. The cleaned and dried gas is discharged through the nozzle 14.

The device has aerators 1о-18 in the form of bubblers, which are located respectively in the lower part of the tank and under its upper extended part. The nutrient medium in the apparatus is fed through the distributor IV, also made in the form of a bubbler. The suspension with microorganisms is removed from the apparatus through fitting 20. Process water is sprayed through the collector nozzles 8 to extinguish the foam.

The device works as follows.

Air or oxygen-enriched air or oxygen is supplied to the lower part of tank 1 through aerators 15, 16 and 17, and due to the difference in the specific gravities of the liquid in the apparatus, vertical circulation of the liquid is established. The upward flow rises in the annular space between the wall of the tank 1 and the cup 4, the downward flow of liquid is lowered into the space between the cups b and 4, and the ascending stream rises inside the cup 3. As the flow moves, gas bubbles disperse through the perforations of the barriers 7. Medium temperature supporting by supplying cooling water to the jacket 2 and the glasses 3 and 4. The plates 5, mounted on the glasses, provide an orderly lubrication of the latter with liquid, which improves heat transfer.

The ascending fluid flows are swirled in a vortex in the upper expanded part of the tank due to the vanes 6. The widened section of the tank 1 and the vortex motion of the fluid contribute to more than complete degassing of the fluid from the exhaust air. The foam formed in the process of growing microorganisms is quenched by the streams of process water supplied to the reservoir 8.

Exhaust gas with liquid particles surrounds the droplet separator 9 and is dried. It then passes through the perforated bottom 11 into the chamber 10 and causes the ion exchange resin to become fluidized. Due to the moisture carried away by the gas stream, the particles of the ion exchange resin swell and carbon dioxide is simultaneously trapped.

from the gas stream in the resultant replacement of the COz ion. The gas, significantly purified from the culture fluid and CO2, can be returned to the apparatus after pre-oxygenation or released into the atmosphere without harming the environment. The ion exchange resin after regeneration and washing is returned to the process cycle. Since the lower part of the tank 1 is made flush, a large volume of liquid

It is located in a zone with increased hydrostatic pressure, and consequently with an increased partial pressure of oxygen, which promotes the growth of aerobic microorganisms.

The centrifugal forces arising from the vortex motion of the fluid and the expansion of the upper part of the downward flow zone improve the partial flotation of microorganisms, as a result of which from the apparatus through the nozzle 20

a more concentrated culture medium is withdrawn.

Claims (4)

1. An apparatus for growing microorganisms, containing a vertically mounted cylindrical container equipped with a lid, a heat exchange jacket, an antifoam and an aerator, and concentrically mounted inside the tank, circulation cups directing, characterized in that, in order to intensify the processes of mass and heat exchange, the apparatus is equipped with a device for desorption of carbon dioxide, reinforced in the lid over the defoamer and made in the form of a chamber filled with an ion exchange resin and having a perforated bottom, under which there is an outlet for supplying gas to the chamber, while the guiding circulation cups are made in the form of coils serving to cool the culture liquid. 2. The apparatus according to claim 1, characterized in that in the inner circulating glass in height, perforated partitions are installed to disperse the gas in the liquid. 3. The apparatus according to claim 1, characterized in that the diameter of the upper and lower parts of the container exceeds the diameter of its middle part, and the outer circulating glass is made in the shape of a funnel. 4. The apparatus according to claim 1, except for the fact that the circulating glasses are equipped with paddles fixed in their upper part. Sources of information taken in the examination at the examination 1. Patent of Great Britain No. 1353008, cl. From 6F, 1974. 2. Patent of the USSR D 290540, cl. From 12V 1/10, 1970. / 7 / Y