SU548996A1 - Virus destruction method - Google Patents

Description

(54). METHOD OF DESTRUCTION OF VIRUSES

one

The invention relates to virology and may find application in the study of mycoplasmal structures in biology and medicine.

A known method of destroying viruses to the scares of exposure to them by physical factors, such as osmotic pressure, hydrodynamic shift, heating 11.

However, the known method does not allow one to judge the nature of the packaging of nucleic acids inside viruses, which makes it difficult to study the conformation of nucleic acids and the structure of nucleoproteins.

The aim of the invention is to preserve

original packaging of viral nucleic acid.

This goal is achieved by the fact that the virus suspension is mixed with the dye and acted upon by a laser with a pulse power of 10 to 100 joules and a wavelength in the heights of the dye absorption band.

The method for destroying viruses is as follows.

Viral suspension, for example bacteriophages Cg and PBV - 6, is mixed with a dye, for example, with green, toluidine blue or Nile blue dye. The concentration of the virus in the mixture should be in the range from Ixio parts per ml

5 d

Claims (1)

dye ranging from 1x10 to IxlO m In this case, the dye adsorbs on the surface of virus envelopes. The mixing time is selected so that the dye molecule also does not have time to diffuse into the virus, for example for; green for 5 hours, and for toluidine blue and Nile blue for 2-3 minutes. Then, the resulting mixture of the virus with the dye is placed in an optical cell in an amount of 1 ml and irradiated with focused monochromatic light from a quantum generator, for example, a GOR-ZO ruby laser, or a laboratory laser system (per rub; che). The irradiation is carried out in the peak mode of the laser, as well as in the free-running mode, when the optical pulse has a relatively smooth shape. The duration of ikhulsa. 1 msec, and power from 3.6 to 100 j. When the mixture is irradiated with light from a laser, the shell of the virus is destroyed, and the necessary condition for destruction is. The selection of the dye in such a way that the light absorption line of the 1-body light includes the laser light emission band. (When a mixture of virus and dye is irradiated, the light energy from the laser is absorbed by dye molecules adsorbed on the surface of the viral envelope, which leads to heating of the cochlean zone and destruction of the envelope. The nucleic acid packed inside the virus when destroyed. The envelope comes out with a compact structure. laxity of the process of the process. The proposed method for the destruction of viruses enables the morphological study of viral nucleic acids to be mainly carried out. The virus inside the virus, without additional purification of the drug from the remnants of the virus envelope. Preserving the shape and packaging of viral ucleic acid using the predicted method anatomically expands the possibilities of studying the conformation of nucleic acids in viruses, the structure of nucleoproteins and chromosomes. viruses by physical factors, such as the fact that, in order to preserve the original packaging of the viral nucleic acid, the suspension of the virus a mixed dye with a laser and act moschnostyuimpulsa from 10 to 100 joules, and a wavelength within the absorption band of the dye. Sources of information taken into account during the examination: 1. Tikhonenko A. S. Ultrastructure of bacteria viruses. M., Medicine, 1968., p. 108