SU536827A1 - The way to restore the vitality of laboratory animals - Google Patents

Description

one

This invention relates to medicine and may find application in clinical practice.

jt-i3BecTeH livelihood recovery method Laorator (Puffed up animals, consisting of cooling dormant and artificial blood supply ij.

However, with the well-known method of restoring the health of the animals, it is possible to reanimate only after short periods of death.

One of the goals of the Discovery is an increase in the period during which the recovery of the organism is possible, as well as the possibility of restoring the vital activity of the organism of small animals.

This is achieved by cooling postmortem at a rate of U, o-, and / OC / min to 1U-ZiC, and artificial blood circulation is carried out by connecting the venous ventral to the inferior vena cava through the left renal vein, and arterial through the tee to artery and vein.

The recovery of live animals of the Laoratory animals is carried out as follows.

Stage 1 - preliminary preparation of the animal.

Under the introductory intravenous thiopental and primary inhalational ether anesthesia (stages; (- ni-i), the isolation and

Intubation of the trachea, followed by the isolation and cannulation of the celiac vein and carotid artery. To the angular artery to measure the pressure of an attachment g mercury manometer. Prior to this, heparin was administered intravenously (U, iU, io ml / kg of body weight; after intravenous administration of relapters of depolarizing effect (e.g. digilin, 2-3 mg / kg of body weight), pulmonary ventilation was started and this condition median longitudinal lanaroomyum is carried out, the left kidney is isolated and its vein is injected with it, through the latter a cannula with a diameter of 4 mm is inserted into the posterior hemisphere, 5 mm in diameter, preliminarily. ) method of outflow through the cannula of the mentioned diameter per minute the bottom passes: at least 300 ml of fluid (blood), the height of the siphon must be at least 25-30 cm, and the length of the cannula is / O cm. From the posterior vena cava the cannula is pulled out through the wall, the discharging cavity is closed tight The animal is placed on the scale and attached to it (electrodes of an electrocardiograph (electrocardioscope) are attached).

11 standard - killing an animal.

artificial respiration is turned off and bleeding is performed: 60-70 ml of blood is evacuated from the posterior vena cava via the cannula introduced earlier. At the same time, blood pressure rapidly decreases to 20 mm Hg. Art., and then to zero. In the next 2-6 minutes, 15–20 agonal respiratory movements are observed, heart activity is disturbed, soon spontaneous breathing and corneal reflex disappear, an isoline appears on the electrocardiogram. Clinical death occurs. 6 minutes In the next 10 minutes, the animal is in a state of death, during which time an electrocardiograph, a manometer and a ventilator are disconnected from the animal.

111 floor cooling of the animal.

After the death of 10 minutes of death (and from the beginning of the bleeding 15-16 minutes), the animal is subjected to artificial cooling. It is carried out in two forms - either by external or viralisovascular cooling of the body.

With external cooling, a bath is prepared in advance, into which cold water is poured and ice is added. Therefore, the water performance is adjusted to. After 10 minutes of death, the animal is immersed in a prepared bath (immersion of a large part of the head in water is necessary). Cooling is fast, especially for the head. To do this, slightly move the water from the outside of the animal.

It is necessary to measure the temperature in the mouth and in the right peep, the gradient between these indicators should not be more. Within 25-30 minutes, the temperature in the mouth drops to 22-23 ° C and in the gut to 24-25 C. Then the animal is removed from the water, wiped dry, fixed to the machine and placed on the scale. Reconnect the electrocardiosa, the device for artificial ventilation of the lungs, the mercury manometer and the previously prepared device for artificial blood circulation, into which 140-160 ml of rabbit donated blood is pre-poured. The blood is pre-oxygenated and, with a new heat exchanger, its temperature is brought to 24 ° C.

With intravascular cooling, a heart-lung machine is prepared in advance, into which 90-100 ml of rabbit blood is poured. This apparatus is attached to cannulas previously inserted into the carotid artery and the vena cava after the onset of death of the animal. Using a heat exchanger, the temperature of the blood in the apparatus is adjusted to 7-8 ° C. After 10 minutes of death (and from the beginning of the r-ovapuskan 15-16 minutes) include the heart-lung machine, the body is perfused with cold venous blood and the intravascular cooling of the animal begins. The volumetric rate of perfusion in this case is i60-70 ml / min. The perfusion is carried out without blood oxygenation. After 20-25 minutes of perfusion, the temperature in the mouth drops to 22-23 ° C, and in the squeeze - to 25-26X; After this, the heart-lung machine is turned off, perfusion and further cooling of the animal is stopped and waited for 25-30 minutes. The animal continues to be in a state of death. During this time, in the switched off cardiopulmonary bypass the blood is pre-oxygenated and warmed with the heat exchanger before.

IV etat - resuscitation.

Resuscitation begins 60 missions after the death of the animal. First, from the apparatus of the heart-lung through the arterial cannula, such an amount of blood that he had withdrawn during bleeding, i.e. 60--70 ml, is evenly metered into the animal. Then a heating pad is placed under the animal, the water temperature in which is 38-40 ° C. Balancing is performed.

After this, the cardiopulmonary apparatus is turned on to remove the clip from the cannula extending from the posterior vena cava. Begin artificial lung ventilation. During the first 15–20 min of perfusion, its volumes are relatively small — 50–70 ml / min. At the subsequent time, the perfusion volumes gradually increase and they are brought to 100, rarely to 120 ml / min. As the body temperature of the animal increases, the temperature of the blood supplied to it gradually increases. At the same time, the gradient between temperatures in the mouth and in the intestine “e should exceed 2-3 ° C. In the experiment, it tends to uniformly warm the entire body of the animal.

After 30-35 minutes from the onset of cardiopulmonary bypass, separate heart contractions appear, then they become rhythmic and effective, blood pressure rises to 60-70 mm and then to 90-100-120 mm Hg. Art. After 60–90 minutes from the onset of artificial blood circulation, the animal occasionally retains its blood pressure at 70–80 mm Hg. Art. by myself. Sometimes a glucose solution (1 ml of 40% solution / kg of body weight) and adrenaline (0.01 ml of 0.1% solution / kg of body weight) is administered to animals to obtain effective cardiac contractions and sufficient blood pressure. The administration of these substances is periodically repeated. If the animal is able to maintain its arterial pressure at a level of 80–90 mm Hg, it is possible for a long time. Art., then its further warming with blood is performed by veno-venous perfusion.

After 1.5-2.0 hours of artificial blood circulation, the animal first appears rare spontaneous respiratory movements, and then spontaneous breathing becomes more frequent. The corneal reflex appears after the third hour of perfusion, the animal begins to respond to pain stimulation (needle pricking) the head turns itself arbitrarily, the corneal reflex steadfastly holds. All of this suggests that the resuscitation effect of a small-sized laboratory animal that is

The total time in the state of death (without breathing and without blood circulation) is clearly 1M.

Formula of invention

Claims (2)

1. The method of restoring the vitality of laboratory animals, which consists in cooling and artificial blood circulation, characterized in that, in order to increase: the period during which the body can be revitalized, the cooling is carried out postmortaly with a speed of 0.5-0.75 ° C / / min to 19-21 ° C. 2. The method according to claim 1, except that, in order to restore the vital activity of the organism of small animals, artificial blood circulation is carried out and connected to the inferior vena cava through the left renal vein, and the arterial through the tee to the artery and to the vein. Sources of information taken BO attention during the examination: 1. Long U. Generator miracles. Proftekhizdat, 1960, p. 380-401.