SU536224A1 - The method of obtaining enzyme preparation for enrichment of feed - Google Patents

The method of obtaining enzyme preparation for enrichment of feed

Info

Publication number
SU536224A1
SU536224A1 SU2121816A SU2121816A SU536224A1 SU 536224 A1 SU536224 A1 SU 536224A1 SU 2121816 A SU2121816 A SU 2121816A SU 2121816 A SU2121816 A SU 2121816A SU 536224 A1 SU536224 A1 SU 536224A1
Authority
SU
USSR - Soviet Union
Prior art keywords
strains
enrichment
feed
cultivation
preparation
Prior art date
Application number
SU2121816A
Other languages
Russian (ru)
Inventor
Степан Михайлович Бакай
Вера Иосиповна Билай
Алла Яковлевна Стрижевская
Original Assignee
Полтавский Научно-Исследовательский Институт Свиноводства
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Полтавский Научно-Исследовательский Институт Свиноводства filed Critical Полтавский Научно-Исследовательский Институт Свиноводства
Priority to SU2121816A priority Critical patent/SU536224A1/en
Application granted granted Critical
Publication of SU536224A1 publication Critical patent/SU536224A1/en

Links

Landscapes

  • Enzymes And Modification Thereof (AREA)

Description

(54) СПОСОБ ПОЛУЧЕНИЯ ФЕРМЕНТНОГО ПРЕПАРАТА ДЛЯ ОБОГАЩЕНИЯ КОРМОВ(54) METHOD FOR OBTAINING AN ENZYME PRODUCT FOR ENRICHING FEED

коэдрыжке образув ксиланазу и с ллюлазу С„ виташшы группы В и щрут е метаболиты, питательна  ереда содержит стержни кукурузы, измельченные до среднего размера частиц 1,0-1,8 мм, увлажненные жидкой кш ералыюй средой 1 : 1, состо щей из аммоний сульфата (N И Л 0,1% и калий дипздрофосфатаКН РО 0,1%, приготовленной на водопроводной воде, рН 4,5-5,5. Посевной материал каждого штамма отдельно выращиваетс  в колбах или Матрицах на стерилизованной среде до 20 суток, а биомасса поверхностным способом в металлических кюветах при 27-28 С с увлажненной аэрацией культивируетс  в течение 3-4 суток. После инкуба1ШИ , смешивани  в соотношении 1 : 1 и высушивани  биомасса двух штаммов (2801 и 1120) содержит ксиланазную активность 40-60 ед/г рибофлавина (Ь,) ДО 19 мг/кг и другие ценные метаболиты. Кроме того, биомасса содержит (% на абсолютно сухое вещество): сырого протеина. 9,48, жира 2,52,клетчатки 29,10, золы 4,60 и безазотистых зксграктивных веществ 54,30. Влажность готового комплесного ферментного препарата составл етCoagulating by forming xylanase and with lulase C, vitashes of group B and other metabolites, the nutrient medium contains corn sticks, crushed to an average particle size of 1.0-1.8 mm, moistened with a liquid xyral medium 1: 1 consisting of ammonium sulfate (N & L 0.1% and potassium diplozidrofosfatKN PO 0.1%, prepared on tap water, pH 4.5-5.5. The seed of each strain is grown separately in flasks or Matrices on sterilized medium for up to 20 days, and biomass surface method in metal cuvettes at 27-28 C with wet aeration to ultrified within 3–4 days.After incubating chi, mixing in a 1: 1 ratio and drying, the biomass of the two strains (2801 and 1120) contains a xylanase activity of 40-60 units / g of riboflavin (L) upto 19 mg / kg and other valuable metabolites In addition, the biomass contains (% per absolutely dry matter): crude protein. 9.48, fat 2.52, fiber 29.10, ash 4.60, and nitrogen-free xxtractive substances 54.30.The moisture content of the prepared complex enzyme preparation is

iai2%.iai2%.

Claims (2)

1.Способ получени  фefJмeнтнoгo препарата , дл  обогащени  кормов, предусматривающий выращивание грибов на питательной среде, содержащей сульфат аммони  и калий дигидрофосфат, отличающийс  тем, что, с целью повьпЦени  ксиланазной активности и биологической ценности препарата, используют штаммы грибов 1. A method of producing femmint preparation for enrichment of forages, involving the cultivation of fungi on a nutrient medium containing ammonium sulfate and potassium dihydrogen phosphate, characterized in that strains are used to determine the xylanase activity and biological value of the preparation Fusarium moniltforme var. lactis Sheld2801 HTrichoderma koninji Oudem 1120, при этом штаммы выращивают раздельно на измeльчeнньLX стержн х кукурузы, предварительно увлажненных питательной средой, затем полученную биомассу смешиваютFusarium moniltforme var. lactis Sheld2801 HTrichoderma koninji Oudem 1120, while the strains are grown separately for crushed LX corn sticks, pre-moistened with a nutrient medium, then the resulting biomass is mixed в соотношении 1 : 1 и высушивают.in the ratio of 1: 1 and dried. 2.Способ :по П.1, о т л и ч а ю щ и и с   тем, что выращивание штаммов осуществл ют при 27 28 С и рН среды 5,5 в течение 4 суток.2. Method: According to claim 1, that is, with the fact that the cultivation of the strains is carried out at 27-28 ° C and a pH of 5.5 for 4 days. Источники информации, прин тые во вниманиеSources of information taken into account при экспертизе:during examination: 1. Патент США Г 3749580, кл 99-9, 31.07.731. US patent G 3749580, CL 99-9, 07/31/73 (прототип).(prototype).
SU2121816A 1975-03-28 1975-03-28 The method of obtaining enzyme preparation for enrichment of feed SU536224A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU2121816A SU536224A1 (en) 1975-03-28 1975-03-28 The method of obtaining enzyme preparation for enrichment of feed

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU2121816A SU536224A1 (en) 1975-03-28 1975-03-28 The method of obtaining enzyme preparation for enrichment of feed

Publications (1)

Publication Number Publication Date
SU536224A1 true SU536224A1 (en) 1976-11-25

Family

ID=20615403

Family Applications (1)

Application Number Title Priority Date Filing Date
SU2121816A SU536224A1 (en) 1975-03-28 1975-03-28 The method of obtaining enzyme preparation for enrichment of feed

Country Status (1)

Country Link
SU (1) SU536224A1 (en)

Similar Documents

Publication Publication Date Title
Taylor The nutritional requirements of the predominant bacterial flora of the soil
Barois et al. Changes in respiration rate and some physicochemical properties of a tropical soil during transit through Pontoscolex corethrurus (Glossoscolecidae, Oligochaeta)
AU724092B2 (en) Quick-fermented feed, its preparation and uses
DE3686597T2 (en) URICASE AND METHOD FOR THE PRODUCTION THEREOF.
US2576932A (en) Fermentation process for production of vitamin b12
DE2409627C3 (en) Production of L-lysine feed concentrate
PT87141B (en) PROCESS FOR THE PREPARATION OF COMPOSITIONS OF BIOFISICALLY TRANSFORMED ASCOMICETES, SCHIZOMICETES AND YEAST, AND VEGETABLE RACES AND NUTRIENTS CONTAINING THESE COMPOSITIONS
SU536224A1 (en) The method of obtaining enzyme preparation for enrichment of feed
Styer Nutrition of the cultivated mushroom
GB1604782A (en) Selection process for obtaining a fungal microorganism of the genus trichoderma having advantageous characteristics
US2766176A (en) Process for culturing anaerobic bacteria
Nygaard Sørensen Dietary fiber and ascorbic acid in white cabbage as affected by fertilization
Lowe et al. Ecological studies on coccoid bacteria in a pine forest soil—II. Growth of bacteria introduced into soil
McCurdy et al. Studies on Stigmatella brunnea
Conn et al. Characteristics of certain bacteria belonging to the autochthonous microflora of soil
DE2164018C3 (en) Process for the biotechnological production of Uncase
DE2363285B2 (en) Process for the production of 1-malic acid from fumaric acid
Nissen Effects of antibiotics on carbon dioxide production in soil
US1309723A (en) Thomas
CN110235822A (en) Living aquatic product Antistress agent and its production method and application method
US4369252A (en) Fermentation process for the preparation of ergot alkaloids, primarily ergocornine and β-ergocryptine
US3261688A (en) Growth promoting agents
DE3137049C2 (en) Method for obtaining uricase using microorganisms
ES8302084A1 (en) Process for the production of penicillin-free mycelium masses from cultures obtained in the production by fermentation of penicillin, and their use as animal feed and fertilizers.
Newton et al. NITRIFICATION UNDER AND AFTER ALFALFA, BROME, TIMOTHY, AND WESTERN RYE GRASS: V. BIOLOGICAL ASSAYS OF HAY CROP RESIDUES