SU506395A1 - Method for toxicological study of animal sa, forcedly scored during fusariotoxicosis - Google Patents

Method for toxicological study of animal sa, forcedly scored during fusariotoxicosis

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Publication number
SU506395A1
SU506395A1 SU2037779A SU2037779A SU506395A1 SU 506395 A1 SU506395 A1 SU 506395A1 SU 2037779 A SU2037779 A SU 2037779A SU 2037779 A SU2037779 A SU 2037779A SU 506395 A1 SU506395 A1 SU 506395A1
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SU
USSR - Soviet Union
Prior art keywords
fusariotoxicosis
forcedly
animal
animals
toxicological study
Prior art date
Application number
SU2037779A
Other languages
Russian (ru)
Inventor
Эльза Мустафовна Хасанова
Юрий Иванович Бойков
Original Assignee
Всесоюзный научно-исследовательский институт ветеринарной санитарии
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Application filed by Всесоюзный научно-исследовательский институт ветеринарной санитарии filed Critical Всесоюзный научно-исследовательский институт ветеринарной санитарии
Priority to SU2037779A priority Critical patent/SU506395A1/en
Application granted granted Critical
Publication of SU506395A1 publication Critical patent/SU506395A1/en

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Description

ют под малым увеличением (7X10) микроскопа и определ ют ,ЦТ,П, токсического экстракта (уславБо1 д ;.кре,стах). Пробирки с  вным пдражением клёто. дюносло  складывают отдельно , остальные пробирки вновь помещают -при темлературе 37°С.they are placed under a small magnification (7X10) of the microscope and the CT, P, toxic extract is determined (uslaBo1; cree, ct). Test tubes with a clear expression of a flute. the dunoslo is folded separately; the remaining tubes are again placed at 37 ° C.

Окончательный учет результатов производитс  через 48 час после внесени  в культуру экстракта. Одновременно просматривают :и контрольные пробирки. Результаты определени  токсичности исследуемого экстракта  вл ютс  достоверными при сохранении моносло  в контроле.A final record of the results is made 48 hours after the culture is added to the extract. At the same time view: and test tubes. The results of the determination of the toxicity of the studied extract are reliable while maintaining the monolayer in the control.

Пример. Из м са овец, вынужденно забитых при различных течени х экспериментального фузариотоксикоза, готовили различные разведени  экстракта на питательной среде и вносили по 1 мл /во флаконы с культурой ткани СПЭВ. Через 24 час производили учет реакции, отметив при этом, что при внесении мышечного экстракта, полученного при хроническом опыте, клетки СПЭВ разрушались в разведений 1 :2000, в то врем  как при подостром опыте - в разведении 1 : 1200. При остром опыте и контроле клетки не разрушались .Example. From sheep's meat, forcedly scored at different currents of experimental fusariotoxicosis, different dilutions of the extract were prepared on a nutrient medium and added 1 ml / into vials of SPEV tissue culture. After 24 hours, the reaction was recorded, noting that when the muscle extract obtained from the chronic experiment was applied, the SPEV cells were destroyed at a dilution of 1: 2000, while at the subacute experience - at a dilution of 1: 1200. With acute experience and control the cells did not break down.

Аналогичные по степени токсичности результаты были получены параллельно на крысах , которым скармливали м со от тех же животных.Similar toxicity results were obtained in parallel in rats fed with animals from the same animals.

Крысы, поедавшие м со животных, забитых при подостром токсикозе, заболевали на 14 день с начала скармливани , а при хроническом токсикозе на 8-10 день. При вскрытии крыс наблюдали патологоморфологические изменени  в паренхиматозных органах. Крысы, поедавшие м со от контрольных животных и животных, забитых при остром токсикозе, оставались живыми. Таким образом, установленна  закономерность позвол ет предложить способ определени  токсичности м са при фузариотоксикозе на культуре ткани СПЭВ и других клетках . Rats that ate m from animals slaughtered with subacute toxicosis, fell ill on day 14 from the beginning of feeding, and in chronic toxemia from 8 to 10 days. At autopsy of the rats, pathological and morphological changes in the parenchymal organs were observed. The rats eating moles from control animals and animals killed in acute toxicosis remained alive. Thus, the established pattern allows us to propose a method for determining the toxicity of mya during fusariotoxicosis on SPEV tissue culture and other cells.

Claims (1)

Формула изобретени Invention Formula Способ токсикологического исследовани  м са животных, вынужденно забитых при фузариотоксикозе , включающий вы вление патологоморфологических -изменений в ткан х паренхиматозных органов животных по дегенеративному изменению в них, отличающийс  тем, что, с целью сОКращени  времени исследовани , испытуемое м со экстрагируют гомогенизированием его в фосфатно-буферном растворе, очищают и полученный экстракт внос т в различных разведени х в монослой культуры клеток паренхиматозных тканей и о степени токсичности м са суд т поA method of toxicological study of animals that were forced to be killed during fusariotoxicosis, including the detection of pathological and morphological changes in the tissues of the parenchymal organs of animals by degenerative changes in them, characterized in that, in order to shorten the time of the study, the test body was extracted with homogenization of his body. buffer solution, it is purified and the resulting extract is applied at various dilutions in a monolayer cell culture of parenchymal tissues and the degree of toxicity of meat is judged by тому, в каком разведении экстракт разрушает ткани.the dilution of the extract destroys the tissue.
SU2037779A 1974-06-10 1974-06-10 Method for toxicological study of animal sa, forcedly scored during fusariotoxicosis SU506395A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU2037779A SU506395A1 (en) 1974-06-10 1974-06-10 Method for toxicological study of animal sa, forcedly scored during fusariotoxicosis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU2037779A SU506395A1 (en) 1974-06-10 1974-06-10 Method for toxicological study of animal sa, forcedly scored during fusariotoxicosis

Publications (1)

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SU506395A1 true SU506395A1 (en) 1976-03-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996988A (en) * 1987-09-22 1991-03-05 Nippon Zoki Pharmaceutical Co., Ltd. Method of determining behavior of drugs in living bodies

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996988A (en) * 1987-09-22 1991-03-05 Nippon Zoki Pharmaceutical Co., Ltd. Method of determining behavior of drugs in living bodies

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