SU490820A1 - The method of differentiation of bacteria - Google Patents
The method of differentiation of bacteriaInfo
- Publication number
- SU490820A1 SU490820A1 SU1838091A SU1838091A SU490820A1 SU 490820 A1 SU490820 A1 SU 490820A1 SU 1838091 A SU1838091 A SU 1838091A SU 1838091 A SU1838091 A SU 1838091A SU 490820 A1 SU490820 A1 SU 490820A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- bacteria
- differentiation
- absorption
- region
- listeria
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
спектр, позвол ющий с высокой степенью достоверности отличить их от д-ругих бактерий. Серогрупповую принадлежность листерий определ ют по наличию или отсутствию поглощени в области 1160 см (диаграмма ЛЬ 1). Эта область поглощени углеводов, которые определ ют серологическую формулу бактерий. У листерий второй серогруппы в этой области наблюдаетс четко выраженное поглощение, а у листерий первой группы поглощение в этой области без границ переходит в полосу 1080 . Вакцинный щтамм «А (перва серогруппа) занимает промежуточное положение, поскольку имеет хорощо выраженное плечо.a spectrum that allows them to be distinguished from other bacteria with a high degree of certainty. Lystery serogroup is determined by the presence or absence of absorption in the region of 1160 cm (L1 diagram). This region is the absorption of carbohydrates, which determine the serological formula of bacteria. In Listeria of the second serogroup, a pronounced absorption is observed in this region, while in Listeria of the first group, absorption in this region without boundaries passes into the 1080 band. The A (serogroup) vaccine vaccine is in an intermediate position because it has a well-defined shoulder.
Место, объект и врем выделени листерий , а также их вирулентность существенным образом не вли ют на ИК-спектры.The location, object and time of release of Listeria, as well as their virulence, do not significantly affect the IR spectra.
4 Предмет изобретени 4 Subject of the invention
Способ дифференциации бактерий путем измерени их абсорбции в инфракрасной области спектра, отличающийс тем, что, с целью обеспечени безопасности при работе с возбудител ми антропозоносных заболеваний , например листери ми, и определени их серологической характеристики, взвесьбактерий автоклавируют не более чем при 1,5 атм в течение 30 мин, нанос т на германиевую пластину в концентрации, обеспечивающий распределение 3-5 млрд. микробных тел на 1 мм поверхности, измер ют абсорбцию взвеси в области от 1250 до см и по наличию максимума поглощени в участке спектра 1160 см суд т об антигенной структуре листерий, позвол ющей отнести их ко второй серогруппе.The method of differentiation of bacteria by measuring their absorption in the infrared region of the spectrum, characterized in that, in order to ensure safety when working with pathogens of anthroposoniferous diseases, such as listeria, and to determine their serological characteristics, bacterial suspension is autoclaved at no more than 1.5 atm per for 30 minutes, applied to the germanium plate in a concentration that provides a distribution of 3-5 billion microbial bodies per 1 mm surface, measure the absorption of the suspension in the region from 1250 to cm and by the presence of a maximum of oshchenilas a portion of the spectrum 1160 cm judged on Listeria antigenic structure allows the guide to carry them to a second serogroup.
CNCN
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1838091A SU490820A1 (en) | 1972-10-19 | 1972-10-19 | The method of differentiation of bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1838091A SU490820A1 (en) | 1972-10-19 | 1972-10-19 | The method of differentiation of bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU490820A1 true SU490820A1 (en) | 1975-11-05 |
Family
ID=20529776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1838091A SU490820A1 (en) | 1972-10-19 | 1972-10-19 | The method of differentiation of bacteria |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU490820A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5660998A (en) * | 1989-02-09 | 1997-08-26 | Bruker Analytik Gmbh | Method for the rapid detection of microorganisms in samples |
-
1972
- 1972-10-19 SU SU1838091A patent/SU490820A1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5660998A (en) * | 1989-02-09 | 1997-08-26 | Bruker Analytik Gmbh | Method for the rapid detection of microorganisms in samples |
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