SU480721A1 - Method for isolating pectolytic enzymes - Google Patents
Method for isolating pectolytic enzymesInfo
- Publication number
- SU480721A1 SU480721A1 SU1950392A SU1950392A SU480721A1 SU 480721 A1 SU480721 A1 SU 480721A1 SU 1950392 A SU1950392 A SU 1950392A SU 1950392 A SU1950392 A SU 1950392A SU 480721 A1 SU480721 A1 SU 480721A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- enzymes
- isolating
- ultrafiltration
- pectolytic enzymes
- membranes
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
(54) СПОСОБ ВЫДЕЛЕНИЯ ПЕКТОЛИТИЧЕСКИХ ФЕРМЕНТОВ(54) METHOD FOR ISOLATING PECTOLYTIC ENZYMES
Пример. Культура плесневого гриба Aspergillus owamori 16 выращиваетс на питательной среде следующего состава, %: сульфат аммони - 0,7, однозамещенный фосфат кали - 0,1, сернокислый магний - 0,05, 3,5%-ньш экстракт свекловичного жома - 95 мл на 100 мл питательной среды и 10%-на выт жка солодовых ростков - 5 мл на 100 мл питательной среды. Длительность роста - 3-е суток, активность культуральной жидкости - 50 ед/мл., содержание сухих веществ - 2,5%, рН 3,0-3,3. После отделени мицели гриба в фильтрат культуральной жидкости, предварительно отсепарированной.Example. The culture of the mold fungus Aspergillus owamori 16 is grown on a nutrient medium of the following composition,%: ammonium sulfate - 0.7, monosodium potassium phosphate - 0.1, magnesium sulphate - 0.05, 3.5% extract of beet pulp - 95 ml per 100 ml of nutrient medium and 10% extract of malt sprouts - 5 ml per 100 ml of nutrient medium. Duration of growth - 3 days, the activity of the culture fluid - 50 units / ml., The solids content - 2.5%, pH 3.0-3.3. After separation of the fungal mycelium into the filtrate of the culture fluid, previously separated.
добавл етс раствор аммиака дл доведени рН до 3,8-4,0, и производитс ультрафильтраци па мембранах из диацетата целлюлозы с размером пор от 40 до 60 А. В результате происходит концентрирование в 10, 20, 30 и более раз. В процессе ультрафильтрации происходит очистка за счет удалени низкомолекул рных веществ. рН полученного концентрата корректируетс раствором аммиака до 4,8-5,3. Фермент осаждаетс при добавлении этанола до концентрации 75°. Осадок отдел етс путем центрифугировани и высушиваетс . Результаты опытов приведены в таблице.ammonia solution is added to bring the pH to 3.8-4.0, and ultrafiltration is performed on membranes from cellulose diacetate with a pore size of 40 to 60 A. As a result, concentration is 10, 20, 30 or more times. In the ultrafiltration process, purification occurs by removing low molecular weight substances. The pH of the concentrate is adjusted with ammonia solution to 4.8-5.3. The enzyme is precipitated by adding ethanol to a concentration of 75 °. The precipitate is separated by centrifugation and dried. The results of the experiments are given in the table.
ТаблицаTable
Вес даетс в грам.чах. Weight is given in grams.
Таким образом, предлагаемый способ получени пектолитических ферментов позвол ет снизить потери ферментативной активности в 3-4 раза и повысить активность «препаратов с 5000 ед/г до 20000 ед/г; степень очистки препаратов повышаетс в 3-4 и более раз. Значительно снижаютс энергозатраты, а также расход органических растворителей, например этанола, в 2 и более раз на осаждение . Длительность процесса сушки сокраш,аетс в 2,2-1,3 раза.Thus, the proposed method for producing pectolytic enzymes reduces the loss of enzymatic activity by 3-4 times and increases the activity of "preparations from 5000 units / g to 20000 units / g; the degree of purification of drugs increases by 3-4 times or more. The energy consumption is significantly reduced, as well as the consumption of organic solvents, for example ethanol, by a factor of 2 or more per precipitation. The duration of the drying process is contracted, 2.2-1.3 times.
Предмет изобретени Subject invention
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1950392A SU480721A1 (en) | 1973-07-25 | 1973-07-25 | Method for isolating pectolytic enzymes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1950392A SU480721A1 (en) | 1973-07-25 | 1973-07-25 | Method for isolating pectolytic enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU480721A1 true SU480721A1 (en) | 1975-08-15 |
Family
ID=20561915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1950392A SU480721A1 (en) | 1973-07-25 | 1973-07-25 | Method for isolating pectolytic enzymes |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU480721A1 (en) |
-
1973
- 1973-07-25 SU SU1950392A patent/SU480721A1/en active
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