SU479782A1 - The method of producing sorbent - Google Patents
The method of producing sorbentInfo
- Publication number
- SU479782A1 SU479782A1 SU1950669A SU1950669A SU479782A1 SU 479782 A1 SU479782 A1 SU 479782A1 SU 1950669 A SU1950669 A SU 1950669A SU 1950669 A SU1950669 A SU 1950669A SU 479782 A1 SU479782 A1 SU 479782A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- sorbent
- water
- producing sorbent
- producing
- meq
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Description
(54) СПОСОБ ПОЛУЧЕНИЯ СОРБЕНТА 12(54) METHOD FOR OBTAINING SORBENT 12
500 мг КМ-целлюлозы (Н+ -форма, 1 мэкв/г) суспендируют ъ 5 мл воды и при перемешивании приливают растворы 86 мг 1,8-диами;нооктана (0,59 люль) ъ 4 мл воды и 250 мг (0,6 моль) п-толуолсульфоната циклогексил-р- N- (N-метилморфолиний) -этилкар бодиимида s 4 мл воды. Суспензию перемешивают 16 час при комнатной температуре. Осадок смолы отдел ют центрифугированием, промывают водой, этиловым спиртом, эфиром и высушивают в вакууме над Р2О5. Выход 490 мг. Содержание азота ,0,535 мэкв/г.500 mg of KM-cellulose (H + form, 1 meq / g) are suspended in 5 ml of water and solutions of 86 mg are added with stirring with 1,8-diam, nooctane (0.59 lul) and 4 ml of water and 250 mg (0 , 6 mol) p-toluenesulfonate cyclohexyl-p-N- (N-methylmorpholinium) -ethylcarbideimide s 4 ml of water. The suspension is stirred for 16 hours at room temperature. The resin precipitates are separated by centrifugation, washed with water, ethyl alcohol, ether, and dried under vacuum over P2O5. Yield 490 mg. Nitrogen content, 0.535 meq / g.
Изотиоцианатное производное 8-аминооктиламида карбоксиметилцеллюлозы синтезируют аналогично п. I. Содержание серы 0,115 мэкв/г.The isothiocyanate derivative of 8-aminooctylamide carboxymethylcellulose is synthesized similarly to item I. The sulfur content is 0.115 meq / g.
III.Синтез изотиоцианата «-аминобензилцеллюлозы провод т аналогично п. I.III. Synthesis of isothiocyanate "-aminobenzylcellulose is carried out similarly to paragraph I.
IV.Ковалентное св зывание пиримидилрибонуклеазы с полученными сорбентами.IV.Covalent binding of the pyrimidylribonuclease with the resulting sorbents.
50 мг изотиоцианата аминоэтилцеллюлозы суспендируют в I мл 0,1 М. боратного буфера (рН 8,7) и приливают 1 мл раствора пиримидилрибонуклеазы (13,4 ое/мл, 280 нм). Смесь50 mg of aminoethyl cellulose isothiocyanate are suspended in I ml of 0.1 M borate buffer (pH 8.7) and 1 ml of pyrimidylribonuclease solution (13.4 ohm / ml, 280 nm) is added. Mixture
перемешивают b час при комнатной температуре . Осадок смолы промывают 2 раза по 1 мл 0,3 М раствором NaCl, водой до отсутстви С1 (по качественной реакции с AgNOs).stirred b hour at room temperature. The resin precipitate is washed with 2 times 1 ml of a 0.3 M solution of NaCl, with water until there is no C1 (by qualitative reaction with AgNOs).
Супернатанты объедин ют и определ ют оптическое поглош,ение при 280 нм. Количество белка, св занного сорбентом, вычисл ют по разности оптических поглошений, оно составл ет 102 мг/г сорбента.Supernatants are pooled and optical attenuation is determined at 280 nm. The amount of protein bound by the sorbent is calculated from the difference in optical absorbances, it is 102 mg / g of sorbent.
Анализ ферментативной активности показал , что активность водонерастворимого фермента составл ет 95-100% от исходной акгивности .Analysis of the enzymatic activity showed that the activity of the water-insoluble enzyme is 95-100% of the initial activity.
Предмет изобретени Subject invention
Способ получени сорбента обработкой целлюлозного материала модифицируюшими агентами, отличающийс тем, что, с целью получени сорбента с высокой ферментативной активностью, в качестве целлюлозного материала используют аминопроизводные простых эфиров целлюлозы и последовательно провод т обработку сероуглеродом при 25-80° СA method of producing a sorbent by treating a cellulosic material with modifying agents, characterized in that, in order to obtain a sorbent with high enzymatic activity, amino-derivatives of cellulose ethers are used as cellulosic material and sequentially treated with carbon disulfide at 25-80 ° C
и изобутилхлорформиатом при 35-40° С.and isobutyl chloroformate at 35-40 ° C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1950669A SU479782A1 (en) | 1973-07-13 | 1973-07-13 | The method of producing sorbent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1950669A SU479782A1 (en) | 1973-07-13 | 1973-07-13 | The method of producing sorbent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU479782A1 true SU479782A1 (en) | 1975-08-05 |
Family
ID=20561983
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1950669A SU479782A1 (en) | 1973-07-13 | 1973-07-13 | The method of producing sorbent |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU479782A1 (en) |
-
1973
- 1973-07-13 SU SU1950669A patent/SU479782A1/en active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Axen et al. | Preparation of modified agarose gels containing thiol groups | |
| US5092992A (en) | Polyethyleneimine matrixes for affinity chromatography | |
| Bayer et al. | [20] Insolubilized biotin for the purification of avidin | |
| US4048416A (en) | Thiopolymers, their derivatives and methods for their preparation and use | |
| US5085779A (en) | Polyethyleneimine matrixes for affinity chromatography | |
| US4286964A (en) | Polyfunctional epoxides and halohydrins used as bridging groups to bind aromatic amine group-containing alcohols and thiols to hydroxyl bearing substrates | |
| DE69007006T2 (en) | Ligand-containing medium for chromatographic separation, process for its preparation and its use for the isolation of synthetic or natural molecules from a fluid mixture. | |
| US3278392A (en) | Water insoluble enzymes | |
| SU479782A1 (en) | The method of producing sorbent | |
| US4808530A (en) | Protein immobilization by adsorption of a hydrophobic amidine protein derivative to a hydrophobic surface | |
| Turková et al. | Immbilization on cellulose in bead form after periodate oxidation and reductive alkylation | |
| EP0403700A1 (en) | Polyethyleneimine matrixes for affinity chromatography | |
| Kennedy et al. | Improvement of the enzyme immobilisation characteristics of macroporous cellulose trans-2, 3-carbonate by pre-swelling in dimethyl sulphoxide | |
| US3833555A (en) | Polysaccharide cyclic carbamate containing compounds | |
| KR950014967B1 (en) | Preparation method of moranoline derivatives | |
| US7084112B2 (en) | Activated polyethylene glycol compounds | |
| SU644760A1 (en) | Method of obtaining immobilized enzymes | |
| SU1479511A1 (en) | Method of producing affinic sorbent | |
| SU1052509A1 (en) | Method for preparing sorbent for immobilizing biologically active substances | |
| SU732278A1 (en) | Method of modifying polysaccharides | |
| SU670567A1 (en) | Method of producing beta-mercaptoethyl-1,3-propylenediamine hydrodichloride | |
| RU2071058C1 (en) | Method of preparing affine sorbent for c-reactive protein isolation | |
| SU952849A1 (en) | 8-(6-aminohexyl)-cyclo-amf-silicochrome as sorbent for biospecific purification of cyclo-amf -binding proteins | |
| RU1836958C (en) | Method for preparation of modelled blood substitute as oxygen carrier | |
| SU883052A1 (en) | Immunosorbent |