SU432189A1 - METHOD OF OBTAINING LITICAL ENZYMES - Google Patents

Description

one

This invention relates to the microbiological industry.

A known method for producing lytic enzymes by cultivating actinomycetes on a nutrient medium containing sources of carbon, nitrogen and mineral salts.

In order to increase the activity of lytic enzymes in the proposed method, actinomycetes belonging to the species griseinus are used, and cultivation is carried out at 43-45 ° C. At the same time from actinomycetes griseinus use strain 11.

In a 30-liter fermenter pour 16 liters of tap water with salt, g:

K2HP0432

MgSO4-7H2O0,8

(NH4) 2SO416

СОСОз16

Yeast64

Yeast autolysate160.

Separately, 160 g of soluble starch is brewed in 1 liter of water and poured into a fermenter. Then the medium is sterilized for 30 min at 120 + 5 ° С, after which the sterilized medium is cooled to 45 ± 3 ° С; The pH of the medium is 6.0 + 0.1; volume of the environment is 16 l. The medium is inoculated with a suspension of the culture of Actinomyces griseinus-11, grown for a day at 43-45 ° C on the same medium. Fermentation is carried out for 18 h g at 44 ± 1 ° C with a constant unmixed} 1em 300 rpm and air supply 1.5 volumes per 1 volume of medium in 1 min. At the end of the fermentation, the activity of the enzymes that dissolve the yeast cell wall in the culture fluid is 8000 units / ml.

The activity of lytic enzymes in the culture fluid is carried out according to the Tabata method.

LA, (- 7-; Мх100Х.

  / t

where LA is lytic activity; f is the degree of dilution of the enzyme preparation;

yo is the initial optical density; dt is the optical density after some time;

d-E is the difference in optical densities of the control sample (before and after the reaction), t is the incubation time 60 min. This item is isolated from Krasnodar soils and has the following characteristics.

Morphology. Sporiferous necrotized, short, arranged by panicles, rod-shaped spores. The culture is colorless, does not form pigments and brown matter. Aerial mycelium is dark gray.

Synthetic environment. On a nitrate medium with sucrose, growth is good, colonies and

the medium is colorless. Aerial mycelium dark gray.

On a nitrate medium with sucrose, growth is good, the colonies and the medium are colorless, the aerial mycelium is gray.

Organic Wednesday. Growth is abundant on starch agar, colonies and medium are not stained. Aerial mycelium is gray-olive. On slices of potato colonies are colorless with a yellowish tinge. The medium is colored and does not grow brown. Gelatinu thins quickly, milk coagulates and neptonizes, starch hydrolyzes quickly. Restores nitrates. Tyrosinase is positive.

The Tresner-Dang environment turns black.

Attitude to carbohydrates. Assimilates glucose, glycerin, sucrose, xylose, arabinose, rhamnose. Antagonism manifests against Gram-positive and Gram-negative bacteria. Suppresses the growth of some fungi and yeast.

The proposed Actinomyces griseinus strain, in contrast to the known ones, has an increased biosynthesis capacity of lytic enzymes, their broad spectrum of action, namely, the yeast cell walls grown on both carbohydrate and hydrocarbon media. In addition, the complex of lytic enzymes of actinomycete consists of active mannonases and glucanases, which determine its catalytic properties.

Subject invention

Claims (2)

1. A method for producing lytic enzymes by cultivating actinomycetes on a nutrient medium containing sources of carbon, nitrogen and mineral salts, characterized in that, in order to increase the activity of enzymes, actinomycetes of the type griseinus are used, and cultivation is carried out at 43-45 ° s 2. A method according to claim 1, characterized in that strain 11 is used from actinomycetes of the griseinus species.