SU426659A1 - METHOD FOR DETERMINING ERYTHROCYTIC ANTIGENS - Google Patents
METHOD FOR DETERMINING ERYTHROCYTIC ANTIGENSInfo
- Publication number
- SU426659A1 SU426659A1 SU1723989A SU1723989A SU426659A1 SU 426659 A1 SU426659 A1 SU 426659A1 SU 1723989 A SU1723989 A SU 1723989A SU 1723989 A SU1723989 A SU 1723989A SU 426659 A1 SU426659 A1 SU 426659A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- determining
- antigens
- erythrocytic antigens
- erythrocytes
- erythrocytic
- Prior art date
Links
Description
1one
Изобретение относитс к области медицины .This invention relates to the field of medicine.
Дл определени эритроцитарных антигенов обычно антисыворотку эритроцитов, предварительно инкубированных с раствором фермента фицина, помещают в обработанные альбумином лунки микрокамеры дл инкубировани смеси.To determine erythrocyte antigens, usually erythrocyte antiserum, previously incubated with ficin enzyme solution, is placed in albumin-treated wells of a microchamber for incubating the mixture.
Цель изобретени - упрощение способа определени эритроцитарных антигенов.The purpose of the invention is to simplify the method for determining erythrocyte antigens.
Предлагаетс использовать эритроциты цельной крови с последующей ферментативной обработкой папаипом под слоем нейтрального масла.It is proposed to use whole blood erythrocytes followed by enzymatic treatment with papaipom under a layer of neutral oil.
Пример. Лунки микрокамеры типа Терасаки заполн ют вазелиновым маслом. Под вазелиновое масло с помощью микропипетки внос т 0,002 мл антисыворотки, 0,002 мл раствора паоаииа и 0,002 мл 2%-ной взвеси эритроцитов , вз тых в собственной сыворотке. Содержимое лунки перемещивают путем осторожного покачивани микрокамеры, и камеру помещают в термостат при 37° С на 30 мин.Example. Microcamera wells of the Terrazaki type are filled with vaseline oil. With a micropipette, 0.002 ml of antisera, 0.002 ml of a paoaia solution and 0.002 ml of a 2% suspension of erythrocytes, taken in the serum, are added under a vaseline oil. The contents of the wells are moved by gently rocking the microchamber, and the chamber is placed in a thermostat at 37 ° C for 30 minutes.
По истечении этого времени каплю из лунки перенос т на предметное стекло и результат реакции читают при увеличении микроскопа в 100 раз. В случае положительной реакции отчетливо видны различной величины агглютинаты , при отрицательной - отдельно лел ащие эритроциты.After this time, a drop from the well is transferred onto a glass slide and the result of the reaction is read 100 times when the microscope is magnified. In the case of a positive reaction, agglutinates of various sizes are clearly visible; in a negative reaction, separately red blood cells are seen separately.
Предлагаемый способ прост в постановке, позвол ет экономить дефицитные и дорогосто щие сыворотки и реагенты, а также дает возможность использовать одип вид камеры дл тестировани всех клеток крови, обеспечива надежность результатов.The proposed method is simple in formulation, it saves scarce and expensive sera and reagents, and also makes it possible to use one kind of chamber for testing all blood cells, ensuring reliable results.
Предмет изобретени Subject invention
Способ определени эритроцитарных антигенов путем смещивани эритроцитов с ферментом и антисывороткой и ипкубировапи полученной смеси, отличающийс тем, что с целью упрощени способа, ферментативной обработке папаином под слоем нейтрального масла подвергают эритроциты цельной крови.The method for determining erythrocyte antigens by displacing erythrocytes with an enzyme and antiserum and using the resulting mixture, characterized in that erythrocytes of whole blood are subjected to enzymatic treatment with papain under a layer of neutral oil.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU1723989A SU426659A1 (en) | 1971-12-13 | 1971-12-13 | METHOD FOR DETERMINING ERYTHROCYTIC ANTIGENS |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU1723989A SU426659A1 (en) | 1971-12-13 | 1971-12-13 | METHOD FOR DETERMINING ERYTHROCYTIC ANTIGENS |
Publications (1)
Publication Number | Publication Date |
---|---|
SU426659A1 true SU426659A1 (en) | 1974-05-05 |
Family
ID=20495927
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU1723989A SU426659A1 (en) | 1971-12-13 | 1971-12-13 | METHOD FOR DETERMINING ERYTHROCYTIC ANTIGENS |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU426659A1 (en) |
-
1971
- 1971-12-13 SU SU1723989A patent/SU426659A1/en active
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