SU406881A1 - MEDIUM FOR CANNING OF TUMOR CELLS - Google Patents
MEDIUM FOR CANNING OF TUMOR CELLSInfo
- Publication number
- SU406881A1 SU406881A1 SU1701538A SU1701538A SU406881A1 SU 406881 A1 SU406881 A1 SU 406881A1 SU 1701538 A SU1701538 A SU 1701538A SU 1701538 A SU1701538 A SU 1701538A SU 406881 A1 SU406881 A1 SU 406881A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- medium
- tumor cells
- canning
- sodium citrate
- saline
- Prior art date
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
1one
Изобретение относитс к области медицины и касаетс консервировани органов и тканей .This invention relates to the field of medicine and concerns the preservation of organs and tissues.
Известна среда дл консервировани опухолевых клеток при пизких температурах, содержаща в 5 объемах среды 20% бычьей сыворотки, 20 глюкозы и 10% диметилсульфоксида .A known medium for preserving tumor cells at avian temperatures, containing in 5 volumes of medium 20% bovine serum, 20 glucose and 10% dimethyl sulfoxide.
Однако така среда обладает высокой токсичностью .However, this environment is highly toxic.
Токсичность предлагаемой среды снижаетс за счет того, что составные компоненты предложенной среды вз ты в следующих соотнощени х , мл: 60%-ный полиэтиленоксид м. в. 400-0,25-0,3 мг, 1,5%-ный цитрат натри 0,2-0,3, физиологический раствор 1,5- 2,5 мл.The toxicity of the proposed medium is reduced due to the fact that the constituent components of the proposed medium are taken in the following ratios, ml: 60% polyethylene oxide m. 400-0.25-0.3 mg, 1.5% sodium citrate 0.2-0.3, physiological solution 1.5-2.5 ml.
И р и м е р. Дл приготовлени среды берут 60%-ный полиэтиленоксид м. в. 400-0,3 мл, 1,5%-ный цитрат натри - 0,3 мл, физиологический раствор из расчета 2 мл на 10 мл асцидиой жидкости.And p and meer. For the preparation of the medium, 60% polyethylene oxide is used. 400-0.3 ml, 1.5% sodium citrate - 0.3 ml, saline at the rate of 2 ml per 10 ml of ascidic liquid.
Среду готов т следующим образом. Указанные ингредиенты, хранимые в стерильном состо нии (физиологический раствор и цитрат натри ), набирают в шприц и производ т забор асцидиой жидкости. Затем полученнуюThe medium is prepared as follows. The above ingredients, stored in a sterile state (saline and sodium citrate), are drawn into a syringe and the ascidic fluid is collected. Then the resulting
смесь Сливают в флакои и добавл ют по капл м 60%-ный полиэтиленоксид м. в. 400 до конечной концентрации 15%. В полученную смесь добавл ют асцидиую жидкость из расчета 10 мЛ на 2 мл физиологического раствора . Замораживание опухолевых штаммов с предлагаемой средой ведут в специальном аппарате программного о.хлаждени поэтапно, т. е. от О до 15°С со скоростью 1°/мин, а затем до -196° со скоростью 200-300°/мин. Оттаивание замороженных опухолевых штаммов провод т быстро на вод ной бане при 39°С. Указанный метод обеспечивает 100%-ную приживл емость после 1 года хранени .the mixture was poured into a bottle and 60% polyethylene oxide was added dropwise. 400 to a final concentration of 15%. Ascidic fluid is added to the resulting mixture at the rate of 10 mL per 2 ml of saline. Freezing of the tumor strains with the proposed medium is carried out in a special apparatus of the software cooling, step by step, i.e. from 0 to 15 ° C at a rate of 1 ° / min, and then to -196 ° at a speed of 200-300 ° / min. Thawing of frozen tumor strains was carried out quickly in a water bath at 39 ° C. This method ensures 100% survival after 1 year of storage.
Предмет изобретени Subject invention
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU1701538A SU406881A1 (en) | 1971-09-24 | 1971-09-24 | MEDIUM FOR CANNING OF TUMOR CELLS |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU1701538A SU406881A1 (en) | 1971-09-24 | 1971-09-24 | MEDIUM FOR CANNING OF TUMOR CELLS |
Publications (1)
Publication Number | Publication Date |
---|---|
SU406881A1 true SU406881A1 (en) | 1973-11-21 |
Family
ID=20489182
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU1701538A SU406881A1 (en) | 1971-09-24 | 1971-09-24 | MEDIUM FOR CANNING OF TUMOR CELLS |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU406881A1 (en) |
-
1971
- 1971-09-24 SU SU1701538A patent/SU406881A1/en active
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