SU406881A1 - MEDIUM FOR CANNING OF TUMOR CELLS - Google Patents

MEDIUM FOR CANNING OF TUMOR CELLS

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Publication number
SU406881A1
SU406881A1 SU1701538A SU1701538A SU406881A1 SU 406881 A1 SU406881 A1 SU 406881A1 SU 1701538 A SU1701538 A SU 1701538A SU 1701538 A SU1701538 A SU 1701538A SU 406881 A1 SU406881 A1 SU 406881A1
Authority
SU
USSR - Soviet Union
Prior art keywords
medium
tumor cells
canning
sodium citrate
saline
Prior art date
Application number
SU1701538A
Other languages
Russian (ru)
Inventor
Н. С. Пушкарь Н. П. Лобасенко витель А. А. Цуцаева
Original Assignee
А. Наконечный , Ю. А. Иткин Украинский институт усовершенствовани врачей
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by А. Наконечный , Ю. А. Иткин Украинский институт усовершенствовани врачей filed Critical А. Наконечный , Ю. А. Иткин Украинский институт усовершенствовани врачей
Priority to SU1701538A priority Critical patent/SU406881A1/en
Application granted granted Critical
Publication of SU406881A1 publication Critical patent/SU406881A1/en

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

1one

Изобретение относитс  к области медицины и касаетс  консервировани  органов и тканей .This invention relates to the field of medicine and concerns the preservation of organs and tissues.

Известна среда дл  консервировани  опухолевых клеток при пизких температурах, содержаща  в 5 объемах среды 20% бычьей сыворотки, 20 глюкозы и 10% диметилсульфоксида .A known medium for preserving tumor cells at avian temperatures, containing in 5 volumes of medium 20% bovine serum, 20 glucose and 10% dimethyl sulfoxide.

Однако така  среда обладает высокой токсичностью .However, this environment is highly toxic.

Токсичность предлагаемой среды снижаетс  за счет того, что составные компоненты предложенной среды вз ты в следующих соотнощени х , мл: 60%-ный полиэтиленоксид м. в. 400-0,25-0,3 мг, 1,5%-ный цитрат натри  0,2-0,3, физиологический раствор 1,5- 2,5 мл.The toxicity of the proposed medium is reduced due to the fact that the constituent components of the proposed medium are taken in the following ratios, ml: 60% polyethylene oxide m. 400-0.25-0.3 mg, 1.5% sodium citrate 0.2-0.3, physiological solution 1.5-2.5 ml.

И р и м е р. Дл  приготовлени  среды берут 60%-ный полиэтиленоксид м. в. 400-0,3 мл, 1,5%-ный цитрат натри  - 0,3 мл, физиологический раствор из расчета 2 мл на 10 мл асцидиой жидкости.And p and meer. For the preparation of the medium, 60% polyethylene oxide is used. 400-0.3 ml, 1.5% sodium citrate - 0.3 ml, saline at the rate of 2 ml per 10 ml of ascidic liquid.

Среду готов т следующим образом. Указанные ингредиенты, хранимые в стерильном состо нии (физиологический раствор и цитрат натри ), набирают в шприц и производ т забор асцидиой жидкости. Затем полученнуюThe medium is prepared as follows. The above ingredients, stored in a sterile state (saline and sodium citrate), are drawn into a syringe and the ascidic fluid is collected. Then the resulting

смесь Сливают в флакои и добавл ют по капл м 60%-ный полиэтиленоксид м. в. 400 до конечной концентрации 15%. В полученную смесь добавл ют асцидиую жидкость из расчета 10 мЛ на 2 мл физиологического раствора . Замораживание опухолевых штаммов с предлагаемой средой ведут в специальном аппарате программного о.хлаждени  поэтапно, т. е. от О до 15°С со скоростью 1°/мин, а затем до -196° со скоростью 200-300°/мин. Оттаивание замороженных опухолевых штаммов провод т быстро на вод ной бане при 39°С. Указанный метод обеспечивает 100%-ную приживл емость после 1 года хранени .the mixture was poured into a bottle and 60% polyethylene oxide was added dropwise. 400 to a final concentration of 15%. Ascidic fluid is added to the resulting mixture at the rate of 10 mL per 2 ml of saline. Freezing of the tumor strains with the proposed medium is carried out in a special apparatus of the software cooling, step by step, i.e. from 0 to 15 ° C at a rate of 1 ° / min, and then to -196 ° at a speed of 200-300 ° / min. Thawing of frozen tumor strains was carried out quickly in a water bath at 39 ° C. This method ensures 100% survival after 1 year of storage.

Предмет изобретени Subject invention

Claims (2)

1.Среда дл  консервировани  опухолевых клеток при низких температурах, отличающа с  тем, что, с целью снижени  токсичности среды , она содержит иолиэтиленоксид м. в. 400, цитрат натри  и физиологический раствор.1. A medium for preserving tumor cells at low temperatures, characterized in that, in order to reduce the toxicity of the medium, it contains ethylene oxide m. 400, sodium citrate and saline. 2.Среда по п. 1, отличающа с  тем, что составные компоненты вз ты в следующих соотношени х , мл: 60%-ный полиэтиленоксид м. в. 400-0,25-0,3 мл; 1,5%-ный цитрат натри  - 0,2-0,3 мл и физиологический раствор 1,5- 2,5 мл. ,2. The medium according to claim 1, wherein the constituent components are taken in the following ratios, ml: 60% polyethylene oxide m. 400-0.25-0.3 ml; 1.5% sodium citrate - 0.2-0.3 ml and physiological solution 1.5-2.5 ml. ,
SU1701538A 1971-09-24 1971-09-24 MEDIUM FOR CANNING OF TUMOR CELLS SU406881A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU1701538A SU406881A1 (en) 1971-09-24 1971-09-24 MEDIUM FOR CANNING OF TUMOR CELLS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU1701538A SU406881A1 (en) 1971-09-24 1971-09-24 MEDIUM FOR CANNING OF TUMOR CELLS

Publications (1)

Publication Number Publication Date
SU406881A1 true SU406881A1 (en) 1973-11-21

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SU1701538A SU406881A1 (en) 1971-09-24 1971-09-24 MEDIUM FOR CANNING OF TUMOR CELLS

Country Status (1)

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