Всесоюзный научно-исследовательский технологический институт антибиотиков медицинского назначения
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FIELD: biotechnology, biochemistry. SUBSTANCE: method of superoxide dismutase isolation from biomass producer Saccharomyces cerevisiae involves the disruption of cells by freezing-thawing, extraction with 0.04-0.06 M potassium-phosphate buffer, pH 7.7-7.9 at the ratio of wet mycelium and extractant = 1: 3, at 7-10 C for 20-24 hr. Before depleted cell mass removal suspension is acidified to pH 4.4 and removal is carried out by centrifugation. Ion-exchange chromatography of supernatant is carried out on carboxylic cation-exchange resin KMT followed by concentration of eluates by ultrafiltration. Gel-chromatography of prepared concentrate is carried out on Sephadex G-75 followed by lyophilization of the end product. Preparation can be used for treatment of joint pathology, pulmonary diseases. EFFECT: improved method of enzyme isolation. 1 tbl
SU4903938/13A1991-01-211991-01-21Method of superoxide dismutase isolation
SU1800841A1
(en)
Structure of the Cell Wall of Straphylococcus aureus, Strain Copenhagen. V. Isolation of Peptidases Active on the Peptide Moiety of the Cell Walls of Some Gram-Positive Bacteria