SU1762934A1 - Composition for sterilization - Google Patents

Abstract

Use - protection of products from damage by microorganisms. The essence of the invention: the composition includes eugenol based essential oils as ingredients and citral with an equal ratio. Ethyl alcohol is used as a solvent. The concentration of the mixture of essential oils in the solvent is 0.5-3.0 vol. %

Description

The invention relates to the protection of materials and products from them from damage by microorganisms and can be used in pharmacology, medicine, light, food, electronic industries, machine tool building, instrument making and other areas of science and technology.

Known compositions for sterilization are based on phenol, formaldehyde, hydrogen peroxide, biocides, fungicides and other chemicals that are widely used in protecting materials and their products from damage by microorganisms (see V.D. Ilyichev, V. Bocharov. , Gorlenko, MV Ecological Basis of Protection against Bio-Injuries. M., Nauka, 1985,264s. :).

A known composition for chemical sterilization is based on resinous products of oxidation of turpentine-oxyterpene solvent, which includes 50% terpenoic alcohols, 40% unsaturated hydrocarbons, 15% phenols, hydroperoxides and acids (see also USSR No. 251768 of 10.09. 69g. MKI A 61 L2 / 18).

However, this composition has low efficiency both in terms of its ability to suppress the viability of various groups of microorganisms at the time of sterilization of products, and in terms of maintaining the duration of its effect on the microflora after applying the composition to the surface of the protected product. In addition, it has a negative effect on materials, causing them to corrode, due to the fact that phenols, hydroperoxides and acids are present, and when using it, protection of the respiratory organs and human hands is necessary.

The purpose of the invention is to increase the sterilization efficiency, prolong the sterile state of the products treated with the composition, and eliminate the negative effect of the composition on humans and materials.

This goal is achieved by using a solution of a mixture of essential oils, based on monoterpenes, in ethanol for sterilization.

Essential oils based on monoterpenes, such as citral, citronelal, geraniol, myrcene, eugenol, carvacrol, cinnamic aldehyde, actively inhibit the growth of microorganisms (see IZeman BE, Smirnov VV , Bondarenko, AS, Phytoncides and Antibiotics of Higher Plants (ed. Naukova Dumka, Kiev, 1984. - 280s.).

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Such high antimicrobial activity of these essential oils is associated with their diverse effects on the cell. For example, in some cases, essential oils block sulfhydric groups of enzymes (see Belous, MA, Pestovsky, I.Ya. About pseudo-allicines, J. Chem., 1950, 20, p. 1701), in others - violate functions of glucose dehydrase, glutamic acid aminferase, and phosphorus metabolism (see, Sarek A. Fitoncidy koreni 1. Cdeleni. - Prum. potrovin, 1955,6, No. 9, p.433-435), thirdly, the respiratory process is separated, protein synthesis, suppress sporulation (see Ayseman, BE, and others. Phytoncides and antibiotics of higher plants. Izd.Naukova, Kiev, 1984, p.171 - 193).

In terms of their physical properties, essential oils are insoluble (partially soluble) in water, oily viscous liquids with a boiling point above 200 ° C. Well soluble in alcohols. They evaporate in the open air, do not leave stains on the paper substrate, they are well absorbed by various materials and do not have a negative effect on them. Safe for humans.

As research has shown, mixtures of various essential oils have particularly high antimicrobial properties, because in this case the properties of the mixture are not a simple sum of the activities of the constituent components, but a new quality that manifests itself in a joint action on the microbial cell. and dependent on the interaction of the constituent compounds, which may be in synergistic or antagonistic ratios.

The stability of the high antimicrobial properties of blends of essential oils is ensured, and for some microorganisms it is even more enhanced by using ethyl alcohol as a solvent, which itself possesses antimicrobial activity. Alcohol is a highly mobile, rapidly evaporating liquid whose boiling point is 78, 39 °. It wets well almost all materials and easily penetrates into macropores, regardless of the degree of their filling with moisture condensate, as it mixes with it in unlimited quantities.

In addition, alcohol, as a solvent of essential oils, does not itself interact with them and interferes with the physicochemical interaction between the components of the mixture of essential oils. Possessing the properties of well wetting various materials and quickly penetrating into their macropores during sterilization of products, alcohol contributes to the uniform distribution of the molecules of the mixture of essential oils throughout

the surfaces of the products being sterilized and the penetration of the latter into the macropores. This subsequently ensures the long-term preservation of sterility of products, since essential oils, having a high boiling temperature, slowly evaporate from their surfaces, suppress the viability of microbes that are deposited from the air.

When sterilized with an alcohol solution of a mixture of essential oils of products, the mechanism of its effect on the microbial cell is divided into several stages. At the first stage, in the process of applying the solution to the surface of the product, its direct contact with the cell occurs, when

0 its outer shell affects the molecules of alcohol and essential oils in the liquid phase. In this case, the alcohol molecules (their large excess) denature the envelope and thereby dramatically increase its permeability for the molecules of essential oils. They penetrate into the microbial cell and act on it through one of the mechanisms described above. At the second stage, after termination of sterilization, molecules of alcohol and a mixture of essential oils in the gas-liquid phase are applied to the cell. In this case, they are deposited on the cell membrane, and since the alcohol is in large excess, its molecules salvage the polypeptides of the membrane, and

Most of them also ensure the free penetration of molecules of the mixture of essential oils into the cell, which leads to its death. The third stage begins after the alcohol has completely evaporated from the surface of the product. In that

In the case of a cell, only molecules of the mixture of essential oils in the gas phase act. This stage is long, since the essential oils have a boiling point of more than 200 ° C, and its duration depends on the conditions in which the product is located. It ensures the preservation of sterility of products for a long time.

Example. Spent sterilization of samples of materials of size 50x100 mm

0 brand alloy D16, MA8, textolite, board radio device size 40x80 m with radio elements (resistors, chips, capacitors, connecting wires). During the sterilization process, the optimal concentrations of the solution of the mixture of essential oils and their ratio in the mixture were determined. To do this, the samples were blown with compressed air for 2 minutes and a solution of a mixture of essential oils in

ethyl alcohol in ratios, by volume: 0.5: 1; 1: 1; 1: 2; 1: 3; 1: 4; 1: 5 concentration of 0.30 ... 3.0%. The blend of essential oils is a citral-based essential oil: citral 96%, monoterpene alcohols 1–2%, monoterpenic hydrocarbons 2–3% and eugenol-based essential oil: eugenol 99%, monoterpene hydrocarbons 0.2 - 0, 3%, monoterpenic alcohols 0,7 - 0,8%.

20 minutes after applying the composition, a batch of samples in the amount of 15 pieces (three samples of each material) were infected with an aqueous suspension of microorganisms according to GOST 9.048-89 and hung out at a climate station on a stand, where it was exposed during the entire warm period of time. After infection, the second batch of samples was wrapped in a wrapping paper and placed in a thermostat, where it was kept for 12 months at a temperature of 29 ± 2 ° C. A third batch of samples was wrapped in sterile plastic bags and placed in a desiccator, at the bottom of which 50 ml of distilled water was poured. The desiccator was placed in a thermostat and kept there at a temperature of 32 ± 2 ° C for 12 months. At the same time, a blank experiment was put in which the same materials were used, but the samples were not treated with a composition for sterilization prior to their infection with a suspension of microorganisms according to GOST 9.048-89.

The control of the onset of biodeterioration of samples of materials by microorganisms, as well as the onset of corrosion, discoloration, and cracking, was carried out visually and with the help of optical means (magnifying glass, microscope) every 10 days of exposure,

The test results are shown in the table, which indicate that the mixture of essential oils in the ratio, by volume, 1: 1 with a concentration of 0.5 to 3.0 vol. % Other ratios and concentrations of necessary biodegradability protected by composition (GOST 9.0 i8-89)

Lada such properties. For example, a mixture of essential oils in a ratio by volume: 1: 2; 0.5: 1; 1: 3; 1: 4; A 1: 5 concentration of less than 0.5% does not possess antimicrobial properties, as a result of which, in almost all samples, the growth of microorganisms is observed already on the 20th day. Mixtures of essential oils in the same ratios, but with a concentration of more than 3.0%, although they have high antimicrobial properties, but because of the excess of essential oils, the processed products quickly become covered with dust, becoming sticky with all the ensuing consequences.

On untreated samples of control, microbial growth was observed: on the climatic site — after 15 days, in the thermostat — on the 8th day, in exiccators — on the 10th day.

The use of this composition for sterilization allows:

improve the efficiency of sterilization products;

eliminate harmful composition on people and materials;

simplify sterilization technology;

Do not remove the composition from the surface of the products during their operation.

The components of the composition are produced by the domestic industry and are inexpensively priced, which makes it possible to put it into practice during the time it has been ordered by the customer.

Claims (1)

Invention Formula A composition for sterilization, including alcoholic solutions of organic compounds, characterized in that, in order to prolong the sterile state of the products treated with the composition and increase safety of use, essential oils based on eugenol and citral are used as ingredients in the composition, Ethyl alcohol is used as a solvent, and the concentration of the mixture of essential oils in the solvent is 0.5-3.0 rpm. essential oils of samples, points