SU1761035A1 - Culture medium for germinating pollen - Google Patents

Abstract

Use: botany, selection and genetic studies on the diagnosis of the viability of pollen. The essence of the invention: pollen is germinated on a nutrient medium containing sucrose, distilled water and a lyophilized extract of germinating seeds. 2 tab.

Description

The invention relates to botany and can be used in a variety of biological and genetic selection studies of angiosperms, when it is necessary to quickly and reliably determine the viability of pollen.

Pollen germination media are known, including distilled water and sucrose at optimal concentrations (1). However, they do not contribute to the maximum germination of pollen grains and the elongation of pollen tubes.

The purpose of the invention is to increase the amount of germinated pollen and the elongation of pollen tubes.

The goal is achieved by those. that the nutrient medium Naumova GF additionally contains a freeze-dried extract of germinating seeds of winter wheat, and the components are taken in the following ratio, wt.%:

Sucrose10-20

Lyophilized

germinating extract

this n0,001-0,00000001

Distilled Water Else

Example. Freshly harvested pollen was germinated in viscous drops of the medium applied to the upper half of the petri dish on the lower half, and distilled water was poured to create optimal moisture. Germination was carried out at an air temperature of +30 - 23 ° C, served as a control - usually distilled water and a solution of sucrose of different concentrations. Experiments were performed in 3 replications with counting after 24 hours of germinated and unsprouted pollen grains in three fields of the microscope, to determine the germination of pollen.

At least 20 pollen tubes were sketched, which were then measured to establish their average length. Knowing these indicators, as well as the maximum length of the pollen tube, determine the pollen germination energy. Statistical processing was carried out by the difference method and the obtained data were compared with the table value.

The results on the germination of pollen on various media are presented in Tables 1 and 2.

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In tab. 1 shows data on the viability of pollen when it is germinated on media with different concentrations of sucrose. The addition of a lyophilized extract of germinating seeds of winter wheat to it increases the viability of pollen, accelerates its germination. The data table. 2 indicate that pollen from different angiosperm plants germinates better and faster on medium containing 20% sucrose and freeze-dried extract of germinating seeds of winter wheat 0.001-0.00000001%, which is confirmed by statistical calculations. This significantly increases the length of the pollen tubes and the percentage of germinated pollen grains.

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Claims (1)

Invention Formula A nutrient medium for germinating pollen, containing distilled water and sucrose, characterized in that, in order to increase the amount of germinated pollen and lengthen pollen tubes, it additionally contains freeze-dried germinating seed extract in the following ratio of wt.%: Sucrose10-20 Lyophilized germinating extract this n0,001-0,00000001 Distilled water Else The percentage of germination (P) and the average length of pollen tubes (L) 12 h after sowing pollen of different plant species reliably at 5% level of significance 20 Table 1 Germination percentage (P) and average length of pollen tubes (L) 12 h after germination of different types of plants  - reliable at 5% level of meaning Table9