SU1726371A1 - Cobalt (ii) hydroselenite (2-water) - Google Patents

Abstract

The invention relates to a new chimical compound, biological active, hidroselenium of cobalt (II) dehydrated with the formula Co (HSeO3)2 ·2H2O,which is used in algobiotechnology to obtain biomass cyanobacteria spiruline with a high concentration of organic fixed selenium which can be used as raw material to obtain pharmaceutical preparations whith selenium.The aim of the invention is to enoreare the quantity of fixed organic selenium in biomass, a new chemical compound is used, hydroselenium cobalt (II) dehidrated.

Description

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The invention relates to a new biologically active chemical compound, cobalt (II) hydroselenite, a dihydrate of the formula CofHSeOate HO, used in algobiotechnology for obtaining biomass of blue-green microalga spirulina with an increased content of organically bound selenium, which can serve as a biological raw material for obtaining selenium-containing drugs .

The closest compound in structure to the present invention is 1Ma25EO3, which is used to produce selenium with biomass.

However, the content of selenium in the biomass of spirulina using sodium selenite is not high enough and varies in the range of 16.2-18.5 mg / 100 g of ABS.

The aim of the invention is to increase the amount of organically bound selenium in the biomass of the blue-green microalgae spirulina.

This goal is achieved by using a new chemical compound, cobalt (II) hydrocelenite dihydrate, as a substance providing an increase in the selenium content in biomass.

Example. Synthesis of cobalt (II) dihydrate hydroselenite.

Selenic acid (H25eOz) and cobalt basic carbonate (llHCoOHJjCOs Basic cobalt (II) carbonate was obtained by mixing aqueous solutions containing the calculated amounts of CoCl and Na 2 CO 2, respectively, to obtain hydroselenite cobalt dihydrate dihydrate).

co VI

removal of foreign ions. Synthesis of Co (NZeOz) 2-2H20 was performed according to the following procedure.

To the warm solution obtained by dissolving 2 g (0.016 mol) of H2 $ eOz in 10 ml of water, 1.22 g (0.008 mol) of freshly prepared (. The resulting solution was filtered and left at room temperature for crystallization was added with constant stirring. After two days, prismatic red crystals fell out of solution, the crystals were filtered off, washed with water and dried in air. The yield of the product was 2.28 g (84%).

Found,%: Co 16.34; Se 44.02 Co (NZeOz) 2-2H20

Calculated,%: With 16.80; Se 45.00 Physical properties of Co (NSeOz) M 350.892 g / mol.

Cobalt (II) hydrocelenite dihydrate is a red prismatic crystal. The substance is stable in air. It is well soluble in water, alcohol, partially in acetonitrile, // eff 5.12 M.B. typical of the octahedral environment of cobalt (II). The presence of two coordinated water molecules was proved on the basis of NMR H spectra (jSHaO 120 MD relative to free water) and thermogravimetric studies. The temperature for removal of two water molecules is L-100-110 ° C. Further decomposition of the complex is observed at | 400 ° C and is accompanied by step release of H20 and 2Se02. The total loss, mass is 75%, which is in good agreement with the scheme

Co (NZeOz) 2-2H20 Co (NZeOz) 2 + 2H20 Co (NZeOz) 2 + CoGOz + 2Se02 + H20

Thus, the above data confirm the formation of cobalt (II) hydroselenite and the presence of two crystallization water molecules.

PRI me R. In a flask with a volume of 0.25 l with a known nutrient medium No. 16 of Gromov of the following composition, g / l; sodium bicarbonate 16.8; potassium hydrogen phosphate 0.5; nitrate

sodium 2.5; potassium sulfate 1.0; sodium chloride 1.0; magnesium sulfate 0.2; calcium chloride 0.04; ferric sulfate 0.01; EDTA 0.08; trace element solution 1.0 ml / l, various concentrations of hydroselenite were added

cobalt (II) dihydrate and spirulina inoculum S. platensis (Nordst.) Geitl at the rate of 0.3-0.4 g / l of dry matter.

Cultivation was carried out in the accumulative mode with periodic stirring, constant illumination of 12–15 thousand erg cm2 – s) on the first day and 18–21 thousand erg / (cm – d | on the following days, at a temperature of 35 ± 2 ° C. After five days of cultivation the algal biomass

separated by filtration, washed with a 1.5% ammonium acetate solution and dried. Selenium in biomass was determined by the method of A.G. Matrosov.

The results of the research

presented in the table.

The use of cobalt (II) dihydrate hydroselenite at a concentration of 10-20 mg / l provides an increase of 87.46-147.35%

the content of selenium in the biomass of spirulina compared with while simultaneously high productivity of spirulina - 1.82-1.85 g / l of ASB.

Study of the state of selenium in biomass,

obtained by the proposed method showed that 52.2-54 / 6% selenium is included in the protein fraction, and 47.8-45.4% is contained in the composition of the low molecular weight fraction

compounds - oligopeptides, amino acids.

Formula of invention

Hydroselenite cobalt (II) is a dihydrate of the formula Co (N3deO3) 2 2H20 as a substance used to obtain the biomass of spirulina with a high content of organically bound selenium.

N E25eOz was used at a concentration that ensures maximum accumulation

selenium -50, 0 mg / l.

Claims (1)

Claim Cobalt (II) hydroselenite is a dihydrate of the formula Co (НЗеОз) ₂ '2Н ₂ О as a substance used to produce spirulina biomass with a high content of organically bound selenium. No. p / p Chemical compound Mg / L concentration Spirulina productivity g / l ASB The content of selenium in biomass, mg / 100 g ASB 1 Co (HSe03) 2 · 2H ₂ O 5,0 1.86 ± 0.032 11.45 ± 0.87 2 Also 10.0 1.83 ± 0.015 34.68 ± 1.45 3 eleven _ 15.0 1.85 ± 0.024 45.76 ± 1.49 4 and 20,0 1.82 ± 0.036 39.61 ± 0.87 5 n 25.0 1.70 ± 0.028 37.31 ± 1.08 6 Na2SeO3 (prototype) 50.0 * 1.76 ± 0.018 18.5 ± 1.46 N agEeO3 was used in a concentration providing a maximum accumulation of selenium of -50.0 mg / L.