SU1724205A1 - Device for introduction of biological material into genitals of animal females - Google Patents

Abstract

The invention relates to the field of animal husbandry, in particular to biotechnology for breeding cattle, and can be used for artificial insemination and embryo transplantation into the reproductive organs of animals. The goal is to expand the functionality by ensuring that both embryos and insemination material are introduced into the female genital tract. The device is designed as a tube 1 placed in a sealed package, one end 3 of which is plugged and has a side opening 4 with an elastic cap 5 inserted into it, and a suction-delivery element 8 in the form of a syringe. A longitudinal inclined chute is made at the location of the side opening along the tube from the plugged end. For artificial insemination of animals, a capillary 5 is removed from the side opening 4 of the tube 1 and the syringe 8 is connected via adapter 7 to the plugged end of the tube 4. g (L

Description

The invention relates to the field of animal husbandry, in particular to the biotechnology of breeding cattle, and can be used in artificial insemination and in the transplantation of embryos into the genital organs of recipients.

A device for non-surgical embryo transfer, containing a metal tube, 45 cm long and 4.5 mm in diameter, in which a plastic urethral catheter 55 cm long and 3 mm in diameter is located, and a suction element in the form of a syringe with an adapter, are known. The device is placed in a protected case to maintain asepsis when inserting the device into the vaginal cavity to the cervical canal.

The catheter is filled with biomaterial immediately before transplantation through the side opening at the blind end of the catheter from a petri dish under a microscope.

A disadvantage of the known device is a small functional possibility - only embryo transfer and the impossibility of successfully performing artificial insemination.

In addition, filling the biomaterial into the catheter of the device causes certain difficulties due to lateral races N5

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the position of the hole in it, "This is especially apparent when the medium with embryos in the petri dish is reduced, as well as when the embryos are located on the bottom of the petri dish below the level of the hole in the catheter

When the device is inserted into the genitals of the animal, the opening in the catheter is filled with mucus with microbes. This reduces the survival rate of embryos.

A device for artificial insemination of animals is known, which contains an elastic tube, the proximal and distal ends of which are turned outwards and fixed on the cylinder with the formation of a closed cavity. In the cavity there is an elastic tubular liner, covered with a layer of antifriction material. In the inner cavity of the tube there is an injection element in the form of an elastic rod to push the sperm. The device provides atraumatic insemination of the female animals.

However, this device has only one functional purpose - artificial insemination and is not suitable for embryo transfer.

It is also known a device for transplanting embryos, comprising a body which is a capillary made of a transparent polymer material, one end of which is bent and made conical, and the other end is plugged and has an opening in the side wall. The suction device includes two elastic cylinders mounted on opposite ends capillary All elements of the device are placed in a sealed package. .

For filling with biomaterial, one elastic bottle is separated in a sealed pocket. At this time, another bladder remains planted on. muffled end of the coopus. Then, the hermetic package is cut from the side of the curved end of the body and the top of the conical part is separated and the biomaterial under the microscope o is collected through the opening. After that, the elastic cylinder is moved to the closed ". end of the body so as to close the side opening, cut off the conical curved part of the body and put on this end the second elastic cylinder.

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For embryo transfer, an elastic balloon is removed from the plugged end and sealed into the (welded) packing part. The plugged end of the body is inserted into the animal's cervix, the cover is pierced and the end of the body is pushed into the uterus horn and the elastic balloon is squeezed out of the body

The described device can transplant embryos and carry out artificial insemination of animals.

However, the known device is complicated in design and maintenance. When cutting the conical part, the edges of the case remain uneven, sharp and can be injured with this end with artificial insemination. Moreover, when cutting the conical part, the material of the case can get into the biomaterial, which is highly undesirable.

When embryos are transplanted, the lateral opening of the body is clogged with microbial-contaminated mucus of the animal's genital tract, which reduces the survival rate of the embryos. The closest to the invention to the technical essence and the effect achieved is an embryo transfer device comprising a tube placed in a sealed package, one end of which is plugged and has a side opening with an elastic capillary inserted into it. This capillary is fixed with a rubber nozzle. The device is equipped with a suction-injection element in the form of a pear impaled on the unobstructed end of the tube.

The biomaterial (embryos with nutrient medium) is refilled through an elastic capillary under a microscope. Then the capillary is removed, and the rubber nozzle closes in this place and seals the opening. For embryo transfer, the rubber nozzle is removed, the plugged end of the tube is inserted into the female genital organs of the animal, and the embryo is pushed out of the tube with a pear.

However, using the device, it is not possible to carry out artificial insemination

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performance, i.e. the device has a narrow functional purpose

The purpose of the invention is to enhance the functionality by ensuring the introduction of embryos and insemination tools into sex animals. ways of females.

The goal is achieved by the fact that in a device for introducing a biomaterial into the genitals of female animals, including a tube placed in a hermetic test, one end is plugged and has a side opening with an elastic pillow inserted into it, and a suction-blower - the longitudinal element according to the invention, on the outer surface of the tube, is a longitudinal sloping groove passing through the side opening, the buried part of which is adjacent to the plugged end of the tube,

Due to the longitudinal chambered gutter, having pulled out the pre-elastic capillary, connect the plugged end of the tube to the suction / discharge element and use the open, unimpressed tube end to fill with sperm and perform artificial insemination.

The proposed trough allows air to pass through the tube and the side opening in it into the suction-injection device, and use the unplugged end of the tube for artificial insemination of A by connecting the suction-pressure element to the ampoule of the tube and inserting the capillary into the side opening using an embryo transfer device

Thus expanded functionality of the device.

When searching through the patent and scientific and technical literature, no technical solutions were found with signs similar to those of the invention, which makes it possible to draw a conclusion that it meets the criterion:

The invention is illustrated.

Fig 1 shows the tube of the device in section without a sealed package; Fig 2 - a section of the tube in the area of the side opening; Fig. 3. shows a complete device assembly without a sealed package when used for artificial insemination; fig 4 is the same for embryo transfer

The device contains a tube 1 with an open unobstructed end 2 and a muffled end 3 Near the muffled end 3, a hole 4 is made in the tube 1, into which elastic is inserted

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Capillary 5, Hole 4 is made at an acute angle to the axis of tube 1 and is directed in the direction opposite to the plugged end 3 to reduce the mucus contaminated by microorganisms from entering the opening 4 when the device is inserted into the genitals of the female animals.

From the side of the side opening 4 (smofigo 1) there is a longitudinal inclined chute 6, the buried part of which is adjacent to the plugged end 3 of the tube 1 o

and. Depending on the specific use of the device, a rubber adapter 7 is put on one of the ends 2 or 3 and a suction-injection unit is attached, for example a syringe 8

The device works as follows

The pre-sterilized device in a sealed 25 plastic bag (not shown in the figures) is transferred to the laboratory for filling with biomaterial "

In case of using the device for artificial insemination up to 30 sterilization, the capillary tube 5 is removed, the adapter 7 is put on the plugged end 3 of the tube 1 and the syringe 8 is attached to the adapter 7 (Fig 3) „

A cover is broken through from the non-terminated end 2 of tube 1 and using squirrel 8 is used to collect sperm into tube 1. Then the sheath is sealed (welded) and the device is transferred to the chick. To inseminate, the device is brought to the genitals of the animal, the sheath is pierced and the tube 1 is inserted into the cervix and the syringe 8 squeezes the sperm out of the tube 1 When the tube 1 is used with sperm, and when the sperm is removed from tube 1, air passes from tube 1 through hole 4, chute 6, adapter .7 into syringe 8 (and back), ensuring the operability of the device. After squeezing the sperm from the tube 1, the device is removed from the genitals of the animal and destroyed

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In the case of embryo transfer, the adapter 7 is put on the end 2 of the tube 1 and the syringe 8 is attached to the adapter 7. At the same time, the capillary 5 finds - s in the hole 4 of the tube 1, the device

placed in a plastic bag and sterilized ",

To fill the device with a biomaterial, a cover is broken from the side of the plugged end 3 of tube 1 and the end of the capillary 5 is inserted through this opening in the cover and using a syringe 8 Type the culture medium into the tube 1 from the Petri dish, then the air column, after which the embryo nutrient medium, then again the air column and the environment column. All manipulations were carried out under a microscope.

After filling the biomaterial into the tube 1, the capillaries p 5 are sealed (brewed or clamped), and also the case is sealed.

Filled with biomaterial device. the property is transported to its destination

For embryo transfer, the capillary 5 is removed from the 4Tubing 1 opening, retracted into the free one: a portion of the sheath and separated by welding, while maintaining tightness,

The device is then inserted with the plugged end 3 of the tube 1 into the vagina of the recipient animal and propelled to the mouth of the cervix. Under rectal control, breaking the cover, the tube 1 is advanced into the horn of the uterus and using the syringe 8 the embryo with Medium is removed from the tube 1 through the side opening 4

After that, the device is removed and destroyed.

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Thus, the proposed device is universal, allowing both artificial insemination of female animals and embryo transfer, which was not achieved in the prototype.

The proposed device is simpler by constitution in comparison with the prototype due to the exclusion of the rubber nozzle fixing the capillary in the side opening, respectively, in operation

Claims (1)

Formula and statement about Device for injecting biomaterial into the hearth organs of female animals, which includes a tube placed in a sealed package, one end of which is plugged and has a side opening with an elastic capillary inserted into it and connected to the tube cavity, a suction-delivery element that is ; This is because, in order to expand the functionality by ensuring that both embryos and insemination material are introduced into the female genitals, a longitudinal oblique trough passing through the side opening is accomplished on the outer surface of the tube. Yv-j 4